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961.
962.
进化历史和气候条件共同影响中国木本植物花色的分布 本研究以中国木本植物为研究对象,主要探讨两个问题:(1)不同生活型物种花色组成的差异;(2)生物地理区、进化年龄和气候条件对不同花色地理分布格局的影响。研究使用7673种木本植物的物种分布数据和花色信息(分为白色、红色、黄色、黄绿色、绿色和蓝紫色),并结合属级系统进化树来比较不同生活型(包括灌木、乔木和藤本)物种花色组成的差异,分析不同生物地理区、进化年龄和现代气候对花色地理格局的影响。研究结果表明,与乔木和藤本植物相比,灌木具有更高比例 的由花青素着色的红色花和蓝紫色花物种。中国木本植物的花色地理格局受到区域效应和现代气候(尤其是降水和UVB辐射)的共同影响。倾向于蜂媒传粉的黄色花和蓝紫色花物种和由花青素着色、耐环境胁迫的红色花和蓝紫色花物种比例在中国西北部地区更高。绿色花物种的进化起源更早,但进化时间对花色地理格局的解释力很弱。这些结果说明中国木本植物花色的地理格局受到进化历史和现代环境的共同影响。  相似文献   
963.
Standard plant DNA barcodes based on 2–3 plastid regions, and nrDNA ITS show variable levels of resolution, and fail to discriminate among species in many plant groups. Genome skimming to recover complete plastid genome sequences and nrDNA arrays has been proposed as a solution to address these resolution limitations. However, few studies have empirically tested what gains are achieved in practice. Of particular interest is whether adding substantially more plastid and nrDNA characters will lead to an increase in discriminatory power, or whether the resolution limitations of standard plant barcodes are fundamentally due to plastid genomes and nrDNA not tracking species boundaries. To address this, we used genome skimming to recover near-complete plastid genomes and nuclear ribosomal DNA from Rhododendron species and compared discrimination success with standard plant barcodes. We sampled 218 individuals representing 145 species of this species-rich and taxonomically difficult genus, focusing on the global biodiversity hotspots of the Himalaya-Hengduan Mountains. Only 33% of species were distinguished using ITS+matK+rbcL+trnH-psbA. In contrast, 55% of species were distinguished using plastid genome and nrDNA sequences. The vast majority of this increase is due to the additional plastid characters. Thus, despite previous studies showing an asymptote in discrimination success beyond 3–4 plastid regions, these results show that a demonstrable increase in discriminatory power is possible with extensive plastid genome data. However, despite these gains, many species remain unresolved, and these results also reinforce the need to access multiple unlinked nuclear loci to obtain transformative gains in species discrimination in plants.  相似文献   
964.
[目的]筛选合适的指标建立一套外来养殖鱼类的生物入侵风险评估体系,并对外来养殖鱼类的生物入侵防控提出对策建议。[方法]通过文献资料的收集和整理,对外来养殖鱼类中典型入侵物种的入侵过程、影响危害和入侵生物学特性进行分析和归纳,从适应能力、繁殖能力、扩散能力3方面指示其入侵性;从对生物的影响和对环境的影响2方面指示其生态影响;从自然因素和人为因素2方面指示环境可入侵性,以上述3方面为框架进行评估体系构建。[结果]筛选20个指标构建了外来养殖鱼类的生物入侵风险评估体系,并举例说明该评估体系的应用。从法规政策、科学研究、治理技术和公众参与等4个方面针对性地提出外来养殖鱼类生物入侵风险防控对策。[结论]防范和治理入侵生物是一个系统工程。对于外来养殖鱼类的管理,既不能只考虑经济效益而置生态风险于不顾,也不能片面放大外来养殖良种的入侵风险。科学管控的关键在于完善制度建设、加强风险评估、发展防治手段、促进公众参与,使外来鱼类养殖业在严格受控的前提下发挥其经济效益,将其潜在的生态危害效应降到最低。  相似文献   
965.
To investigate the nanomechanics of the erythrocyte membrane we developed a hybrid model that couples the actin-spectrin network to the lipid bilayer. This model features a Fourier space Brownian dynamics model of the bilayer, a Brownian dynamics model of the actin protofilament, and a modified wormlike-chain model of the spectrin (including a cable-dynamics model to predict the oscillation in tension). This model enables us to predict the nanomechanics of single or multiple units of the protein network, the lipid bilayer, and the effect of their interactions. The present work is focused on the attitude of the actin protofilament at the equilibrium states coupled with the elevations of the lipid bilayer through their primary linkage at the suspension complex in deformations. Two different actin-spectrin junctions are considered at the junctional complex. With a point-attachment junction, large pitch angles and bifurcation of yaw angles are predicted. Thermal fluctuations at bifurcation may lead to mode-switching, which may affect the network and the physiological performance of the membrane. In contrast, with a wrap-around junction, pitch angles remain small, and the occurrence of bifurcation is greatly reduced. These simulations suggest the importance of three-dimensional molecular junctions and the lipid bilayer/protein network coupling on cell membrane mechanics.  相似文献   
966.
Ligustrum lucidum Ait. has been used in traditional Chinese medicine for over 1000 years because of its anti-tumor, antimutagenic, antidiabetic, and hepatoprotective properties. The aim of this study was to determine whether oleanolic acid (OA) is the principal active compound of L. lucidum responsible for its antidiabetic properties, and to examine its effect on the expression of thyroid hormones and insulin secretion, thus revealing the mechanism by which L. lucidum modulates insulin levels in diabetes. When rats with streptozotocin-induced diabetes were treated with OA (100 and 200 mg/kg body mass per day, for 40 days), the changes in blood glucose levels and in oral glucose tolerance tests showed that hypoglycemia was more pronounced in OA-treated groups than in the diabetic control rats, and that the levels of triglyceride, total cholesterol, and low-density lipoportein cholesterol in OA-treated rats were lower than those in the diabetic control rats, whose high-density lipoprotein cholesterol increased. OA-treated rats also gained weight, and exhibited increased serum insulin levels. In contrast, OA treatment did not effect the levels of thyroid hormone or TSH in rats with streptozotocin-induced diabetes. These results indicate that OA has hypoglycemic and hypolipidemic effects. OA treatment might stimulate insulin release, and consequently, results in the modulation of glucose levels and regulation of lipid metabolism.  相似文献   
967.
Cui X  Gao DY  Fink BF  Vasconez HC  Rinker B 《Cryobiology》2007,55(3):295-304
Despite advances in cryobiology, the reliable cryopreservation of complex tissues has not yet been achieved. This study evaluates the viability of cryopreserved composite flaps and demonstrates the feasibility of their transplantation. Epigastric flaps were harvested from male Lewis rats. 1.5 M dimethyl sulfoxide (Me2SO) was used as the initial cryoprotectant agent (CPA). Samples were frozen at controlled rate to −140 °C and transferred to liquid nitrogen for at least two weeks. Hematoxylin and eosin (H/E) staining, MTT tetrazolium salt assay, and factor VIII immunostaining were used to evaluate the overall histology, epithelial viability, and vascular endothelial integrity, respectively, of cryopreserved flaps. For the in vivo phase, flaps were isotransplanted to 35 recipient animals, divided into three groups: fresh (n = 10), perfused (n = 8), and cryopreserved (n = 17). Blood vessel patency was assessed via Doppler at 1, 7, and 60 days post-transplantation. For in vitro studies, cryopreserved samples (10/10) retained normal cell architecture and vascular endothelial integrity upon H/E and factor VIII staining. The viability index of cryopreserved composite flap skin (n = 10) was 11.17 ± 2.01, which was not significantly different from fresh controls (n = 10, 12.15 ± 1.32). All transplanted flaps in the fresh and perfusion groups survived with healthy color and hair growth at 60 days after operation. Survival in the cryopreserved group ranged from 2 to 60 days, with a mean of 12 days. These results demonstrate that the long term survival of cryopreserved composite tissue transplants is possible. Further studies are needed to refine protocols for the reliable cryopreservation of composite parts.  相似文献   
968.
Conjugated linolenic acids (CLN) refer to a group of octadecatrienoic acids with three conjugated double bonds. Minor positional and geometrical differences among CLN isomers make their separation and identification difficult. We have used GC-MS and NMR to study three common CLN isomers namely alpha-eleostearic acid, beta-eleostearic acid and punicic acid, finding that some signals of olefinic carbon atoms in NMR spectra were mistakenly assigned in the literature. The present study was therefore undertaken to re-characterize the location of CC double bonds and assign the chemical signals of proton and carbon atoms using (1)H NMR, (13)C NMR, (1)H-(1)H two-dimensional correlation spectra ((1)H-(1)H COSY) and (13)C-(1)H two-dimensional correlation spectra ((13)C-(1)H COSY). The geometrical structure of double bonds in these three CLN isomers was identified using homonuclear decoupling technique.  相似文献   
969.
970.
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