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121.
Diversity of halophilic microorganisms: Environments,phylogeny, physiology,and applications 总被引:13,自引:0,他引:13
Oren A 《Journal of industrial microbiology & biotechnology》2002,28(1):56-63
The phylogenetic diversity of microorganisms living at high salt concentrations is surprising. Halophiles are found in each
of the three domains: Archaea, Bacteria, and Eucarya. The metabolic diversity of halophiles is great as well: they include
oxygenic and anoxygenic phototrophs, aerobic heterotrophs, fermenters, denitrifiers, sulfate reducers, and methanogens. The
diversity of metabolic types encountered decreases with salinity. The upper salinity limit at which each dissimilatory process
takes place is correlated with the amount of energy generated and the energetic cost of osmotic adaptation. Our understanding
of the biodiversity in salt-saturated environments has increased greatly in recent years. Using a combination of culture techniques,
molecular biological methods, and chemotaxonomic studies, we have obtained information on the nature of the halophilic Archaea
as well as the halophilic Bacteria that inhabit saltern crystallizer ponds. Several halophilic microorganisms are being exploited
in biotechnology. In some cases, such as the production of ectoine, the product is directly related to the halophilic behavior
of the producing microorganism. In other cases, such as the extraction of β-carotene from Dunaliella or the potential use of Haloferax species for the production of poly-β-hydroxyalkanoate or extracellular polysaccharides, similar products can be obtained
from non-halophiles, but halophilic microorganisms may present advantages over the use of non-halophilic counterparts. Journal of Industrial Microbiology & Biotechnology (2002) 28, 56–63 DOI: 10.1038/sj/jim/7000176
Received 20 May 2001/ Accepted in revised form 20 June 2001 相似文献
122.
从皖南尖吻蝮蛇(Agkistrodonacutus)毒液中经DEAE-Sepharose和SephacrylS-200两步凝胶柱层析首次纯化出一种中分子量出血毒素(简称AaHⅣ).经SDS-PAGE和等电聚焦凝胶电泳测定其分子量为44kD,等电点为pH5.0.从500mg粗毒中可获得20mgAaHⅣ纯品.AaHⅣ有较强的出血活性,最小出血剂量(MHD)为0.4μg. 相似文献
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Chloroplasts are believed to be descendants of ancestral cyanobacteria that had peptidoglycan layer between the outer and the inner membranes. Historically, the glaucophyte Cyanophora paradoxa and the rhizopod Paulinella chromatophora were believed to harbor symbiotic cyanobacteria having peptidoglycan, which were conventionally named “cyanelles”. In addition, the complete set of genes involved in the synthesis of peptidoglycan has been found in the moss Physcomitrella patens and some plants and algae. The presence of peptidoglycan-like structures was demonstrated by a new metabolic labeling technique in P. patens. However, many green algae and all known red algae lack peptidoglycan-related genes. That is the reason why we questioned the origin of peptidoglycan-synthesizing enzymes in the chloroplasts of the green algae and plants. We performed phylogenetic analysis of ten enzymes involved in the synthesis of peptidoglycan exploiting the Gclust homolog clusters and additional genomic data. As expected, all the identified genes encoded in the chromatophore genome of P. chromatophora were closely related to cyanobacterial homologs. In the green algae and plants, only two genes, murA and mraY, were found to be closely related to cyanobacterial homologs. The origins of all other genes were diverse. Unfortunately, the origins of C. paradoxa genes were not clearly determined because of incompleteness of published genomic data. We discuss on the probable evolutionary scenarios to explain the mostly non-cyanobacterial origins of the biosynthetic enzymes of chloroplast peptidoglycan: A plausible one includes extensive multiple horizontal gene transfers during the early evolution of Viridiplantae. 相似文献
125.
Priya Padmanabhan Mukund R. Shukla J. Alan Sullivan Praveen K. Saxena 《Plant Cell, Tissue and Organ Culture》2017,128(1):145-160
C4 plants can efficiently accumulate CO2 in leaves and thus reduce wasteful oxygen fixation by the RuBisCO enzyme. Three C4 enzymes, namely carbonic anhydrase (CA), phosphoenol pyruvate (PEPC) and pyruvate orthophosphate dikinase (PPDK), were over expressed in Oryza sativa L. ssp. indica var. Khitish under the control of green tissue specific promoters PD54o, PEPC and PPDK, respectively. Integration of these genes was confirmed by Southern hybridization. The relative expression of PEPC, CA and PPDK were, respectively, 6.75, 6.57 and 3.6-fold higher in transgenic plants compared to wild type plants (control). Photosynthetic efficiency of the transgenic plants increased significantly along with a 12?% increase in grain yield compared to wild type plants. Compared to control plants, transgenic plants also showed phenotypic changes such as increased leaf blade size, root biomass, and plant height and anatomical changes such as greater leaf vein number, bundle sheath cells, and bulliform cells. Our findings indicate that the combined over expression of these three enzymes is an efficient strategy for incorporating beneficial physiological and anatomical features that will enable subsequent yield enhancement in C3 rice plants. 相似文献
126.
127.
籼粳亚种间杂交稻米脂肪含量的遗传分析 总被引:4,自引:0,他引:4
用包括基因型×环境互作效应的种子性状遗传模型,研究了籼粳亚种间杂交稻米脂肪含量的遗传特性,结果表明:在籼粳杂种中,脂肪含量的遗传表达主要受控于种子直接加性效应和母体加性效应,以前者为主.基因型X环境互作主要表现为显性(包括直接显性和母体显性)X环境以及细胞质X环境工作.直接近传率和母体遗传率都极显著.此外,根据遗传效应预测值对供试条本的利用价值作了评价. 相似文献
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129.