全文获取类型
收费全文 | 17234篇 |
免费 | 1515篇 |
国内免费 | 1705篇 |
出版年
2024年 | 56篇 |
2023年 | 267篇 |
2022年 | 698篇 |
2021年 | 1001篇 |
2020年 | 714篇 |
2019年 | 845篇 |
2018年 | 781篇 |
2017年 | 517篇 |
2016年 | 777篇 |
2015年 | 1118篇 |
2014年 | 1375篇 |
2013年 | 1322篇 |
2012年 | 1620篇 |
2011年 | 1436篇 |
2010年 | 888篇 |
2009年 | 790篇 |
2008年 | 955篇 |
2007年 | 828篇 |
2006年 | 674篇 |
2005年 | 572篇 |
2004年 | 453篇 |
2003年 | 393篇 |
2002年 | 285篇 |
2001年 | 266篇 |
2000年 | 237篇 |
1999年 | 285篇 |
1998年 | 172篇 |
1997年 | 174篇 |
1996年 | 129篇 |
1995年 | 104篇 |
1994年 | 105篇 |
1993年 | 76篇 |
1992年 | 99篇 |
1991年 | 80篇 |
1990年 | 64篇 |
1989年 | 61篇 |
1988年 | 46篇 |
1987年 | 48篇 |
1986年 | 40篇 |
1985年 | 40篇 |
1984年 | 20篇 |
1983年 | 16篇 |
1982年 | 8篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1978年 | 3篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
42.
本文报道了在AppleⅡ型微机上实现核酸数据处理的一系列工作程序。应用这些程序,可进行核酸数据的贮存、对指定的核酸数据结构的改造、限制性内切酶识别位点的检索、核酸序列至蛋白序列的翻译、相关核酸序列及蛋白序列的同源性比较、氨基酸密码使用频率的统计和基因的启动子结构的初步探索等方面的工作。 相似文献
43.
Yan Yongshan Qian Jin Xi Xiahui 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,70(5):529-535
Summary Two 30 g/ml BrdU-resistant sublines and two 60 g/ml BrdU-resistant sublines are induced from a Chinese hamster cell line Wg3h (HGPRT–) by one-step and two-step selections, respectively. By inoculating the cells into BrdU-free medium or by adding more BrdU into the culture medium for 26–27 h, it was found that the two BrdU-resistant sublines analysed have very clear sister chromatid differential (SCD) staining patterns. This indicates that some of the nuclear DNA of the BrdU-resistant cells incorporate with BrdU to reach a kinetic balance. Frequencies of sister chromatid exchange (SCE) of the resistant cells are twice to four times as high as those of the Wg3h cells, depending on which BrdU-resistant subline is analysed. The SCE frequencies of the resistant cells also increase with the BrdU concentration in the medium. Analysis of silver-stained nucleolar organizer regions (NORs) indicates that the NOR activity of three out of the four BrdU-resistant sublines is significantly suppressed, i.e., averages of the Ag-NOR number and number of the chromosomes bearing Ag-NORs per cell decrease significantly. The degree of suppression for different BrdU-resistant sublines may be quite different. The suppressed NOR activity of the resistant cells can gradually be restored when the cells are inoculated into BrdU-free medium, but the recovery speed is far lower than that of the Wg3h cells. The suppression of the NOR activity of the BrdU-resistant sublines should be due to BrdU toxicity. 相似文献
44.
团花的种子中有抑制物质,把种子和果实的其它部分分别用有机溶剂提取和分离,所得粗提物经柱层析和薄层层析纯化后,用气相色谱、高效液相色谱、气-质联用等仪器进行测定,并结合生物鉴定,证明种子和果实其它部分中均含有一定量的脱落酸。种子中的含量为11.7μg/g干重,其它部分15.8μg/g干重。 相似文献
45.
46.
Structure and synthesis of histopine, a histidine derivative produced by crown gall tumors 总被引:2,自引:0,他引:2
Histopine, an unusual amino acid derivative of histidine isolated from crown gall tumors of sunflowers (Helianthus annus) inoculated with Agrobacterium tumefaciens strain B6, was previously assigned the gross structure N-(1-carboxyethyl) histidine (2). A diastereomeric mixture containing histopine (2a and 2b) was readily prepared by reductive alkylation of (S)-histidine (1) with pyruvic acid and sodium cyanoborohydride. The individual diastereomers were prepared by reaction of (S)-histidine with (R)- and (S)-2-bromopropionic acid. (R)-N-(1-Carboxyethyl)-(S)-histidine (2a) supports the growth of A. tumefaciens whereas (S)-N-(1-carboxyethyl)-(S)-histidine (2b) is inactive. Therefore, we assign structure 2a to histopine. 相似文献
47.
Sprague-Dawley albino rats or Long-Evans pigmented rats were exposed during the dark phase of the daily light:dark cycle to various intensities of a sunlight-stimulating white fluorescent light (0.022, 0.044, 0.110, 0.220, 0.440 or 2.200 μW/cm2) for 30 min; pineal glands and trunk blood samples were then collected and assayed for melatonin by radioimmunoassay. Albino rats exposed to irradiances of 0.110 μW/cm2 or less had pineal melatonin levels that were not significantly different from those of unexposed animals; higher irradiances significantly (P < 0.001) reduced melatonin levels. In contrast, as little as 0.022 μW/cm2 significantly (P < 0.02) reduced pineal and serum melatonin levels in the pigmented rats. These results suggest that something other than the simple presence or absence of eye pigmentation is the critical factor in determining the sensitivity of the rat's pineal to retinal-mediated photic suppression of melatonin synthesis. 相似文献
48.
Rats were treated with highly purified gossypol acetic acid at doses of 15 or 30 mg/kg day-1 for 6 weeks to produce an effect on spermatogenesis as shown by reduced sperm motility and increased sperm malformation rates. The treated rats did not differ from the controls in the body weight growth curves and reproductive organ weights. When stimulated with hCG, testicular blood flow was increased in the low dose group; the testosterone concentrations in peripheral and testicular venous blood were also increased to a greater extent than those of the control group. No difference was found between the high dose and control groups in testicular blood flow or testosterone concentrations. The morphology of the Leydig cells was apparently normal, although some degenerative changes in the germinal epithelium were observed in the high dose group. Therefore, there is no evidence in our experiment to show any anti-androgenic effect following 6-week treatment of gossypol in rats, even at the dose of 30 mg/kg day-1. 相似文献
49.
50.
在现代社会中,人类的经济建设以与自然过程相竞争的规模进行着,所造成的生态影响不仅表现在地区生态系统的结构和功能上,也表现在生物圈中,而且还从地球化学循环中显示出来。这种情况,使得现代社会的经济管理,必然由传统的经济管理逐步转变成生态经济管理。 相似文献