Genetic diversity and population differentiation in the yellow drum Nibea albiflora along the coast of the China Sea

Nibea albiflora (yellow drum) is an important seafood fish species in East Asia. We explored the population genetic variation of N. albiflora along the coastal waters of the China Sea using microsatellite markers to facilitate a selective breeding programme that is undertaken in China. A total of 256 alleles were detected at 12 loci in four wild populations. A high level of genetic diversity was observed with the mean number of alleles and the observed and expected heterozygosity in each population ranging from 7.917 to 14.083, 0.701 to 0.764 and 0.765 to 0.841, respectively. Pairwise fixation index (F_ST) analysis indicated significant but weak genetic differentiation among populations from four localities (F_ST?=?0.030, P?<?0.01), which was also confirmed by analysis of molecular variance (AMOVA). Significant genetic differentiation was detected between Ningde and the other populations (F_ST?=?0.047–0.056, P?<?0.01). Structure analysis suggested that N. albiflora within the examined range might be composed of two stocks. The data of the present study revealed high genetic diversity and low genetic differentiation among the N. albiflora populations along the coast of the China Sea. This baseline information could be valuable for future selective breeding programmes of N. albiflora.     

Clinical Features of Brain Metastases in Small Cell Lung Cancer: an Implication for Hippocampal Sparing Whole Brain Radiation Therapy

PURPOSE: To assess the clinical features and distribution of brain metastases (BMs) of small cell lung cancer (SCLC) in the hippocampal and perihippocampal region, with the purpose of exploring the viability of hippocampal-sparing whole-brain radiation therapy (HS-WBRT) on reducing neurocognitive deficits. METHODS: This was a retrospective analysis of the clinical characteristics and patterns of BMs in patients with SCLC. Associations between the clinical characteristics and hippocampal metastases (HMs)/perihippocampal metastases (PHMs) were evaluated in univariate and multivariate regression analyses. RESULTS: A total of 1594 brain metastatic lesions were identified in 180 patients. Thirty-two (17.8%) patients were diagnosed with BMs at the time of primary SCLC diagnosis. The median interval between diagnosis of primary SCLC and BMs was 9.3 months. There were 9 (5.0%) and 22 (12.2%) patients with HMs and PHMs (patients with BMs located in or within 5 mm around the hippocampus), respectively. In the univariate and multivariate analysis, the number of BMs was the risk factor for HMs and PHMs. Patients with BMs ≥ 5 had significantly higher risk of HMs (odds ratio [OR] 7.892, 95% confidence interval [CI] 1.469-42.404, P = .016), and patients with BMs ≥ 7 had significantly higher risk of PHMs (OR 5.162, 95% CI 2.017-13.213, P = .001). Patients with extracranial metastases are also associated with HMs. CONCLUSIONS: Our results indicate that patients with nonoligometastatic disease are significantly associated with HMs and PHMs. The incidence of PHMs may be acceptably low enough to perform HS-WBRT for SCLC. Our findings provide valuable clinical data to assess the benefit of HS-WBRT in SCLC patients with BMs.     

木薯己糖激酶MeHXK5的催化功能鉴定

该研究利用ISSR分子标记,对分布于福建省内5个样地( 邵武、建阳、建瓯、周宁和屏南)的61个野钩锥(Castanopsis tibetana)单株的遗传多样性进行了分析,并采用聚类分析方法探讨了它们的遗传关系。结果表明： 用10条ISSR引物从61个单株的基因组DNA共扩增出158条带,包含145条多态性条带,多态性条带百分率达91.77%,其中引物 UBC817、UBC819与UBC842的多态性条带百分率（PPB）为100.0%。各居群的多态性条带百分率(PPB)、有效等位基因数(Ne)、Nei’s基因多样度(H)和Shannon’s多样性指数（I）等各遗传指数差异较大,其中各项遗传指标中最高的为邵武居群,而周宁居群则最低。5个居群的基因分化系数和基因流分别为0.144 0和2.973 0,说明5个居群总遗传变异的14.40%存在于居群间,85.60%存在于居群内。种间总基因多样度分别为0.395 8,种内基因多样度分别为0.338 8,表明钩锥种间遗传多样性较高,且种间变异大于种内变异。各居群间的遗传距离差异较大; 其中,邵武与建瓯居群的遗传距离最近,仅为0.081 5; 建阳和周宁居群的遗传距离最远,为0.162 9。通过聚类分析可将5个钩锥居群聚为3支,屏南与周宁的居群各自独立聚为2支;来自邵武、建瓯及建阳的居群聚为一支,且可进一步分为两个亚支,建阳居群为1个亚支,邵武和建瓯居群聚为1个亚支。供试的钩锥具有较高的遗传多样性,存在着较为频繁的基因交流。该研究结果较准确地揭示了钩锥种间的遗传多样性。     

Proteomic Comparison of Two-Dimensipnal Gel Electrophoresis Pro- files from Human Lung Squamous Carcinoma and Normal Bronchial Epithelial Tissues

Differential proteome profiles of human lung squamous carcinoma tissue compared to paired tumor-adjacent normal bronchial epithelial tissue were established and analyzed by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and matrix-assisted laser desorp-tion/ionization time of flight mass spectrometry (MALDI-TOF-MS). The results showed that well-resolved, reproducible 2-DE patterns of human lung squamous carcinoma and adjacent normal bronchial epithelial tissues were obtained under the condition of 0.75-mg protein-load. The average deviation of spot position was 0.733±0.101 mm in IEF direction, and 0.925±0.207 mm in SDS-PAGE direction. For tumor tissue, a total of 1241±88 spots were detected, 987±65 spots were matched with an average matching rate of 79.5%. For control, a total of 1190±72 spots were detected, and 875±48 spots were matched with an average matching rate of 73.5%. A total of 864±34 spots were matched between tumors and controls. F     

Changes in actomyosin ATP consumption rate in rat diaphragm muscle fibers during postnatal development.

Early postnatal development of rat diaphragm muscle (Dia(m)) is marked by dramatic transitions in myosin heavy chain (MHC) isoform expression. We hypothesized that the transition from the neonatal isoform of MHC (MHC(Neo)) to adult fast MHC isoform expression in Dia(m) fibers is accompanied by an increase in both the maximum velocity of the actomyosin ATPase reaction (V(max) ATPase) and the ATP consumption rate during maximum isometric activation (ATP(iso)). Rat Dia(m) fibers were evaluated at postnatal days 0, 14, and 28 and in adults (day 84). Across all ages, V(max) ATPase of fibers was significantly higher than ATP(iso). The reserve capacity for ATP consumption [1 - (ratio of ATP(iso) to V(max) ATP(ase))] was remarkably constant ( approximately 55-60%) across age groups, although at day 28 and in adults the reserve capacity for ATP consumption was slightly higher for fibers expressing MHC(Slow) compared with fast MHC isoforms. At day 28 and in adults, both V(max) ATPase and ATP(iso) were lower in fibers expressing MHC(Slow) followed in rank order by fibers expressing MHC(2A), MHC(2X), and MHC(2B). For fibers expressing MHC(Neo), V(max) ATPase, and ATP(iso) were comparable to values for adult fibers expressing MHC(Slow) but significantly lower than values for fibers expressing fast MHC isoforms. We conclude that postnatal transitions from MHC(Neo) to adult fast MHC isoform expression in Dia(m) fibers are associated with corresponding but disproportionate changes in V(max) ATPase and ATP(iso).     

降纤酶治疗肺心病难治性心衰疗效观察

目的　探讨降纤酶治疗肺心病难治性心衰的临床疗效和安全性。　方法　将 64例肺心病难治性心衰患者随机分为治疗组和对照组 ,全部病例按常规治疗 ,治疗组加用降纤酶 5 u加入 0 .9%氯化钠 15 0 ml中静滴 ,每天 1次 ,监测心功能、血氧饱和度及血液流变学指标 ,取治疗 7天后的临床资料进行评价。　结果　临床总有效率治疗组 90 .6% ,明显高于对照组的 68.8% (χ2 =4.73 ,P <0 .0 5 ) ,无严重不良反应 ,治疗前后心功能、血氧饱和度及血液流变学指标比较 ,治疗组优于对照组 ,两组具有显著或非常显著性差异 (P <0 .0 5或 P <0 .0 1)。　结论　降纤酶能明显改善肺心病难治性心衰患者的心、肺功能 ,安全有效     

Anion channels influence ECC by modulating L-type Ca(2+) channel in ventricular myocytes.

Anion channels are extensively expressed in the heart, but their roles in cardiac excitation-contraction coupling (ECC) are poorly understood. We, therefore, investigated the effects of anion channels on cardiac ventricular ECC. Edge detection, fura 2 fluorescence measurements, and whole cell patch-clamp techniques were used to measure cell shortening, the intracellular Ca(2+) transient, and the L-type Ca(2+) current (I(Ca,L)) in single rat ventricular myocytes. The anion channel blockers 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) and niflumic acid reversibly inhibited the Ca(2+) transients and cell shortening in a dose-dependent manner. Comparable results were observed when the majority of the extracellular Cl(-) was replaced with the relatively impermeant anions glutamate (Glt(-)) and aspartate (Asp(-)). NPPB and niflumic acid or the Cl(-) substitutes did not affect the resting intracellular Ca(2+) concentration but significantly inhibited I(Ca,L). In contrast, replacement of extracellular Cl(-) with the permeant anions NO, SCN(-), and Br(-) supported the ECC and I(Ca,L), which were still sensitive to blockade by NPPB. Exposure of cardiac ventricular myocytes to a hypotonic bath solution enhanced the amplitude of cell shortening and supported I(Ca,L), whereas hypertonic stress depressed the contraction and I(Ca,L). Moreover, cardiac contraction was completely abolished by NPPB (50 microM) under hypotonic conditions. It is concluded that a swelling-activated anion channel may be involved in the regulation of cardiac ECC through modulating L-type Ca(2+) channel activity.     

铜绿紫球藻(Porphyridium aerugineum)755培养的研究

紫球藻是一种具有潜在应用价值的经济微藻,它的生长速率受各种环境因子的影响.本文研究了不同光强、光周期、盐度、NaHCO3浓度、葡萄糖浓度、培养方式等因子对铜绿紫球藻(Porphyridium aerugineum)755生长的影响,并确定其最佳的培养条件.此外还分析了该藻的吸收光谱,为其培养提供参考数据.     

甘肃党河下游地区早渐新世哺乳动物化石的发现

描述了产于党河下游地区的狍牛泉组下部地层中的叮当沟哺乳动物群。化石共计 1 1属 1 4种 :Palaeoscaptorcf.P .acridens、Palaeoscaptorsp .、Oligosciurusdangheensisgen .etsp .nov.、Tataromyscf.T .sigmodon、Karakoromysdecessus、Coelodontomysasiaticus、Parasminthusspp .、Cricetidaegen .etsp .indet.、Desmatolagusgobiensis、D .pusillus、Allaceropssp .、Schizotheriumordo sium和Parabrachyodussp .。党河鲜松鼠 (新属、新种 )的主要特征是个体较小而原始 ,下颌骨咬肌窝前缘达m2下方 ;冠状突前缘近于垂直 ,起于m2后部下方 ,颊齿齿冠很低 ,臼齿具明显的下内尖、下次脊和下中附尖 ,但无下中尖。上述化石的已知时代分布表明 ,狍牛泉组下部的叮当沟哺乳动物群的时代为早渐新世