全文获取类型
收费全文 | 244篇 |
免费 | 8篇 |
专业分类
252篇 |
出版年
2022年 | 2篇 |
2021年 | 7篇 |
2020年 | 2篇 |
2018年 | 8篇 |
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 8篇 |
2014年 | 12篇 |
2013年 | 14篇 |
2012年 | 10篇 |
2011年 | 14篇 |
2010年 | 9篇 |
2009年 | 19篇 |
2008年 | 22篇 |
2007年 | 17篇 |
2006年 | 18篇 |
2005年 | 14篇 |
2004年 | 11篇 |
2003年 | 7篇 |
2002年 | 3篇 |
2001年 | 7篇 |
2000年 | 2篇 |
1999年 | 7篇 |
1998年 | 5篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 3篇 |
1965年 | 1篇 |
排序方式: 共有252条查询结果,搜索用时 15 毫秒
121.
Samra Hafeez Mahwish Urooj Shamiala Saleem Zeeshan Gillani Sumaira Shaheen Mahmood Husain Qazi Muhammad Imran Naseer Zafar Iqbal Shakeel Ahmed Ansari Absarul Haque Muhammad Asif Manzoor Ahmad Mir Ashraf Ali Peter Natesan Pushparaj Mohammad Sarwar Jamal Mahmood Rasool 《PloS one》2016,11(1)
This study has been undertaken to explore the therapeutic effects of deguelin and specific siRNAs in HeLa cells. The data provided clearly show the silencing of ERK 1/2 with siRNAs and inhibition of ERK1/2 with deguelin treatment in HeLa cells. Additionally, we are providing information that deguelin binds directly to anti-apoptotic Bcl-2, Bcl-xl and Mcl-1 in the hydrophobic grooves, thereby releasing BAD and BAX from dimerization with these proteins. This results in increased apoptotic activity through the intrinsic pathway involved in rupture of mitochondrial membrane and release of cytochrome C. Evidence for inhibition of ERK1/2 by deguelin and escape of BAD phosphorylation at serine 112 through ERK/RSK pathway has been further fortified by obtaining similar results by silencing ERK 1/2 each with specific siRNAs. Increase in BAD after treatment with deguelin or siRNAs has been interpreted to mean that deguelin acts through several alternative pathways and therefore can be used as effective therapeutic agent. 相似文献
122.
Based on morphology, the species status and taxonomic affinities of three species of Tinospora (T. cordifolia, T. sinensis, and T. crispa) with ranges in India, have been questioned. To evaluate species delimitation and population structure among 40 accessions of the three species, a relatively new marker, cytochrome P450, was used. Five out of nine primers generated polymorphisms with 39 out of 47 bands polymorphic. The P450 binary data, when analyzed using distance methods, strongly supported the monophyly of each Indian species and were congruent with previous RAPD work. To further investigate the status of these species, we combined P450 and RAPD data. The resulting unrooted phylogram highly supports the monophyly of each species but with little population structure within each species. To understand the phylogenetic placement of the three Indian Tinospora species within Menispermaceae, chloroplast atpB and rbcL sequence data for a large sampling of the family were analyzed using likelihood and parsimony methods. The resulting phylogenies highly support the Indian Tinospora species as part of a clade (expanded Tinosporeae), consisting of diverse Menispermaceae from around the world. The three Indian species are monophyletic and are most closely related to Tinospora species from Australia (T. esiangkara and T. smilacina). 相似文献
123.
Ishtiaq Ahmad Abid Mehmood Ahmed Khurshid Wajahat M. Qazi Daniel C. Hoessli Evelyne Walker‐Nasir Abdul Rauf Shakoori Nasir‐ud‐Din 《Journal of cellular biochemistry》2009,108(1):64-74
Phosphorylation, one of the most common protein post‐translational modifications (PTMs) on hydroxyl groups of S/T/Y is catalyzed by kinases and involves the presence or absence of certain amino acid residues in the vicinity of the phosphorylation sites. Using MAPRes, we have analyzed the substrate proteins of Phospho.ELM 7.0 and found that there are both general and specific requirements for the presence or absence of particular amino acids in the vicinity of phosphorylated S/T/Y for both of the phosphorylation data, whether or not kinase information was taken into account. Patterns extracted by MAPRes for kinase‐specific data have been utilized to find the consensus sequence motifs for various kinases required to catalyze the process of phosphorylation on S/T/Y. These consensus sequences for different kinase groups, families, and individual members are consistent with those described earlier with some novel consensus reported for the first time. A comparison study for the patterns mined by MAPRes with the results of existing prediction methods was performed by searching for these patterns in the vicinity of phosphorylation sites predicted by different available method. This comparison resulted in 87–98% conformity with the results of the predictions by available methods. Additionally, the patterns mined by MAPRes for substrate sites included 61 kinases, the highest number analyzed so far. J. Cell. Biochem. 108: 64–74, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
124.
Nasier Ahmad Sarojini Johri Malik Z. Abdin Ghulam N. Qazi 《World journal of microbiology & biotechnology》2009,25(1):107-113
The bacterial diversity in the forest soil of Kashmir, India was investigated by 16S rDNA-dependent molecular phylogeny. Small
subunit rRNA (16S rDNA) from forest soil metagenome were amplified by polymerase chain reaction (PCR) using primers specific
to the domain bacteria. 30 unique phylotypes were obtained by PCR based RFLP of 16S rRNA genes using endonucleases Hae 111 and Msp 1, which were most suitable to score the genetic diversity. The use of 16S rRNA analysis allowed identification of several
bacterial populations in the soil belonging to the following phyla: Firmicutes (33.3%), Bacteroidetes (13.3%), Proteobacterium (6.6%), Planctomycete (3.3%), and Deferribacteraceae (3.3%) in addition to the others that were not classified, beyond Archaea domain, However, 36.6% of the retrieved bacterial
sequences could not be grouped with any phylum/lineage. The large amount of unclassified clone sequence could imply that novel
groups of bacteria were present in the forest soil. 相似文献
125.
Shahid Mansoor Javaria Qazi Imran Amin Abdullah Khatri Imtiaz A. Khan Saboohi Raza Yusuf Zafar Rob W. Briddon 《Molecular biotechnology》2005,30(2):167-169
Banana bunchy top disease is a major constraint to banana production in most regions where this crop is grown. The disease
is caused by Banana bunchy top virus (BBTV), a multicomponent, single-stranded DNA virus of the family Nanoviridae. We have designed primers to a conserved region of the master replication-associated protein that are useful for the polymerase
chain reaction (PCR)-mediated detection of BBTV. In addition, primers to banana genomic sequence are used as an internal control,
overcoming the uncertainty (owing to false-negatives) inherent in PCR diagnostics. Together these primer sets are a valuable
tool in the effort to control BBTV, particularly in screening micropropagated banana plantlets for the absence of virus before
release to farmers. 相似文献
126.
Calcium‐permeable AMPA receptors and silent synapses in cocaine‐conditioned place preference
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Myrto Panopoulou Peter A Neumann Seth GN Grant M Foster Olive Yan Dong Oliver M Schlüter 《The EMBO journal》2017,36(4):458-474
Exposure to cocaine generates silent synapses in the nucleus accumbens (NAc), whose eventual unsilencing/maturation by recruitment of calcium‐permeable AMPA‐type glutamate receptors (CP‐AMPARs) after drug withdrawal results in profound remodeling of NAc neuro‐circuits. Silent synapse‐based NAc remodeling was shown to be critical for several drug‐induced behaviors, but its role in acquisition and retention of the association between drug rewarding effects and drug‐associated contexts has remained unclear. Here, we find that the postsynaptic proteins PSD‐93, PSD‐95, and SAP102 differentially regulate excitatory synapse properties in the NAc. Mice deficient for either of these scaffold proteins exhibit distinct maturation patterns of silent synapses and thus provided instructive animal models to examine the role of NAc silent synapse maturation in cocaine‐conditioned place preference (CPP). Wild‐type and knockout mice alike all acquired cocaine‐CPP and exhibited increased levels of silent synapses after drug‐context conditioning. However, the mice differed in CPP retention and CP‐AMPAR incorporation. Collectively, our results indicate that CP‐AMPAR‐mediated maturation of silent synapses in the NAc is a signature of drug–context association, but this maturation is not required for establishing or retaining cocaine‐CPP. 相似文献
127.
Nathan Scott Catherine B Reynolds Michael J Wright Omar Qazi Neil Fairweather Mahendra P Deonarain 《BMC biotechnology》2008,8(1):1-10
Background
Progress in generating comprehensive EST libraries and genome sequencing is setting the stage for reverse genetic approaches to gene function studies in the blacklegged tick (Ixodes scapularis). However, proving that RNAi can work in nervous tissue has been problematic. Developing an ability to manipulate gene expression in the tick synganglia likely would accelerate understanding of tick neurobiology. Here, we assess gene silencing by RNA interference in the adult female black-legged tick synganglia.Results
Tick β-Actin and Na+-K+-ATPase were chosen as targets because both genes express in all tick tissues including synganglia. This allowed us to deliver dsRNA in the unfed adult female ticks and follow a) uptake of dsRNA and b) gene disruption in synganglia. In vitro assays demonstrated total disruption of both tick β-Actin and Na+-K+-ATPase in the synganglia, salivary glands and midguts. When dsRNA was microinjected in unfed adult female ticks, nearly all exhibited target gene disruption in the synganglia once ticks were partially blood fed.Conclusion
Abdominal injection of dsRNA into unfed adult female ticks appears to silence target gene expression even in the tick synganglia. The ability of dsRNA to cross the blood-brain barrier in ticks suggests that RNAi should prove to be a useful method for dissecting function of synganglia genes expressing specific neuropeptides in order to better assess their role in tick biology. 相似文献128.
Thota N Koul S Reddy MV Sangwan PL Khan IA Kumar A Raja AF Andotra SS Qazi GN 《Bioorganic & medicinal chemistry》2008,16(13):6535-6543
Monoterpene citral and citronellal have been used as starting material for the preparation of 5,9-dimethyl-deca-2,4,8-trienoic acid amides and 9-formyl-5-methyl-deca-2,4,8-trienoic acid amides. The amides on bioevaluation as efflux pump inhibitors (EPIs) against Staphylococcus aureus 1199 and NorA overexpressing S. aureus 1199B bacteria resulted in the identification of several of these as potent EPIs. Many of these amides have been shown to possess potency higher or equivalent to known EPIs such as reserpine, verapamil, carsonic acid, and piperine. In this communication, we report a convenient synthesis of alkenyl amides, their bioevaluation and identification as efflux pump inhibitors against S. aureus. 相似文献
129.
The gfp (green fluorescent protein) gene has previously been used to construct a variety of reporter plasmids for Gram-positive bacteria
for bacterial localization and gene expression studies. When a native red-shifted gfp variant (gfp3) was cloned into an expression vector using the P
xyn
promoter and used to transform the soil-borne pathogen Listeria monocytogenes, only a small proportion of the population was seen to fluoresce when examined by epifluorescence microscopy. When the P
xyn
promoter was replaced with the P
xylA
promoter, with accompanying modification of the translation initiation region of the gfp3 gene, a homogeneously fluorescent population of cells was obtained. When expressed in other Gram-positive organisms, such
as Staphylococcus aureus and Bacillus subtilis, the translationally enhanced gene also resulted in high-level and homogeneous GFP production within the bacterial population.
High-level expression of these reporter constructs in L. monocytogenes was evaluated to determine if it had any detrimental biological effect during intracellular infection of eukaryotic cell
lines. The gfp3
+
Listeria were found to invade equally as well as the wild-type cells; showing that these expression systems can be used to monitor
the bacterium in natural environments. Based on these results, similar translationally enhanced vectors were also developed
using unstable GFP3 variants, which retain their short-half life characteristics in L. monocytogenes and therefore can be used as a sensitive monitor of gene expression. 相似文献
130.
A strain ofMicrococcus sp. was isolated for its capability of side chain degradation of cholesterol. This strain was characterized and identified
asMicrococcus roseus. It was found to be the best strain for the production of androsta-1,4-diene-3, 17-dione and androst-4-ene-3, 17-dione compared
with otherMicrococcus strains. 相似文献