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Zhao Shan Qinglin Han Jia Nie Xuezhi Cao Zuojia Chen Shuying Yin Yayi Gao Fang Lin Xiaohui Zhou Ke Xu Huimin Fan Zhikang Qian Bing Sun Jin Zhong Bin Li Andy Tsun 《The Journal of biological chemistry》2013,288(49):35093-35103
Although lysine methylation is classically known to regulate histone function, its role in modulating antiviral restriction factor activity remains uncharacterized. Interferon-induced transmembrane protein 3 (IFITM3) was found monomethylated on its lysine 88 residue (IFITM3-K88me1) to reduce its antiviral activity, mediated by the lysine methyltransferase SET7. Vesicular stomatitis virus and influenza A virus infection increased IFITM3-K88me1 levels by promoting the interaction between IFITM3 and SET7, suggesting that this pathway could be hijacked to support infection; conversely, IFN-α reduced IFITM3-K88me1 levels. These findings may have important implications in the design of therapeutics targeting protein methylation against infectious diseases. 相似文献
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Jinfei Yan Rubin Cheng Xiangzhi Lin Song You Ke Li Hui Rong Yong Ma 《Applied microbiology and biotechnology》2013,97(5):1933-1939
High acetate accumulation was produced during glucose fermentation in high cell density cultures, which is harmful to cell growth. In order to reduce the negative impact of acetate accumulation on the fermentation products, we introduced the Escherichia coli acetyl-CoA synthetase (ACS) gene into the marine microalga Schizochytrium sp. TIO1101, generating genetically modified ACS transformants. The results of PCR and blotting analyses showed that the exogenous ACS gene was incorporated into the genome and successfully expressed. The engineered Schizochytrium increased the pH value and reduced the acetate concentration in the final fermentation medium significantly. Furthermore, the ACS transformants exhibited faster growth and glucose consumption rates than the wild-type strain. The biomass and fatty acid proportion of ACS transformants increased by 29.9 and 11.3 %, respectively. Taken together, the data suggest that ACS overexpression in Schizochytrium might improve the utilization of carbon resource and decrease the production of acetate byproduct. These results demonstrate that application of ACS in metabolic genetic engineering could improve the properties of Schizochytrium significantly. 相似文献
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Ramachandran Prakasam Mitsuaki Fujimoto Ryosuke Takii Naoki Hayashida Eiichi Takaki Ke Tan Fangxu Wu Sachiye Inouye Akira Nakai 《FEBS letters》2013
The febrile response is elicited by pyrogenic cytokines including IL-6 in response to microorganism infections and diseases in vertebrates. Mammalian HSF1, which senses elevations in temperature, negatively regulates the response by suppressing pyrogenic cytokine expression. We here showed that HSF3, an avian ortholog of mammalian HSF1, directly binds to and activates IL-6 during heat shock in chicken cells. Other components of the febrile response mechanism, such as IL-1β and ATF3, were also differently regulated in mammalian and chicken cells. These results suggest that the febrile response is exacerbated by a feed-forward circuit composed of the HSF3-IL-6 pathway in birds. 相似文献
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Ke Xing Guang-Kai Bian Sheng Qin Hans-Peter Klenk Bo Yuan Yue-Ji Zhang Wen-Jun Li Ji-Hong Jiang 《Antonie van Leeuwenhoek》2013,104(6):1245-1245
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