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排序方式: 共有137条查询结果,搜索用时 15 毫秒
31.
32.
Interaction of duramycin with artificial and natural membranes 总被引:6,自引:0,他引:6
Duramycin is a polypeptide antibiotic (molecular weight 2012) obtained from culture filtrates of Streptomyces cinnamomeus forma azacoluta. In this work, we show that low concentrations of duramycin induced aggregation of lipid vesicles containing unsaturated phosphatidylethanolamine and unsaturated monogalactosyl diglyceride, and of sarcoplasmic reticulum vesicles from rabbit skeletal muscle. Furthermore, duramycin inhibited the ATP-dependent Ca2+ uptake in sarcoplasmic reticulum vesicles without affecting the hydrolysis of ATP or the permeability of Ca2+. Also, duramycin only inhibited the bacteriorhodopsin proton pump reconstituted into phospholipid vesicles containing phosphatidylethanolamine. We have isolated a duramycin-resistant strain of Bacillus subtilis and have mapped the location of duramycin resistance. In this strain, the secretion of protons and influx of calcium were resistant to duramycin, and its lipid composition was profoundly different from that of the parent strain. No phosphatidylethanolamine was detected in the resistant strain. Our findings are consistent with the idea that duramycin recognizes a particular membrane conformation determined by the presence of phosphatidylethanolamine or monogalactosyl diglyceride. 相似文献
33.
34.
Yao-Te Huang Chung-Hung Liu Yao-Chih Yang Ritu Aneja Su-Ying Wen Chih-Yang Huang Wei-Wen Kuo 《Journal of cellular physiology》2019,234(8):13557-13570
The prevalence of chronic hyperglycemia and its complications, imposing a critical burden on the worldwide economy and the global healthcare system, is a pressing issue. Mounting evidence indicates that oxidative stress and hypoxia, two noticeable features of hyperglycemia, play a joint crucial role in mediating cellular apoptosis. However, the underlying detailed molecular mechanism remains elusive. Triggered by the observation that insulin-like growth factor (IGF1)-binding protein 3 (IGFBP3) can mediate, in renal cells, high-glucose-induced apoptosis by elevating oxidative stress, we wish to, in this study, know whether or not the similar scenario holds in cardiac cells and, if so, to find its relevant molecular key players, thereby dissecting the underlying molecular pathway. Specifically, we used a combination of three different cellular sources (H9c2 cells, diabetic rats, and neonatal rat ventricular cardiomyocytes) as our model systems of study. We made use of Co-IP assay and western blot analysis in conjunction with loss-of-function reasoning, gain-of-function logic, and inhibitor treatment as our main analytical tools. As a result, briefly, our main findings are that hyperglycemia can induce cardiac IGFBP3 overexpression and secretion, that high levels of IGFBP3 can sequester IGF1 from IGF1 survival pathway, leading to apoptosis, and that IGFBP3 gene upregulation is hypoxia-inducible factor (HIF)1α-dependent and reactive oxygen species dependent. Piecing these findings together allows us to propose the improved molecular regulatory mechanism. In conclusion, we have established the molecular roles of IGFBP3, HIF1, and prolyl hydroxylase domain in connecting oxidative stress with hypoxia and in cellular apoptosis under hyperglycemia. 相似文献
35.
Phage phiC31 integrase-mediated genomic integration and long-term gene expression in the lung after nonviral gene delivery 总被引:1,自引:0,他引:1
BACKGROUND: Phage phiC31 integrase has emerged as a potent tool for achieving long-term gene expression in different tissues. The present study investigated the activity of phiC31 integrase in murine lungs. METHODS: Transfections in murine alveolar epithelial (MLE12) cells were performed with Lipofectamine 2000. For in vivo gene delivery, DNA was complexed with polyethylenimine (PEI) and PEI-DNA complexes were injected intravenously into mice. Expression of luciferase in mice was monitored by in vivo bioluminsecence imaging. Genomic integration and integration into a previously described 'hotspot' were confirmed by polymerase chain reaction (PCR). RESULTS: phiC31 integrase mediated intramolecular recombination between wild-type attB and attP sites in MLE12 cells. Long-term gene expression could be observed in MLE12 cells in the presence of integrase without any selection pressure. Long-term expression of luciferase after intravenous injection of PEI-DNA complexes could be observed only in the lungs of mice which were co-injected with the integrase-encoding plasmid. Increased amounts of integrase plasmid and administration of a second dose had no effect on the level of luciferase expression achieved with a single dose, which was three orders of magnitude lower than the values observed on 'day 1' post application. Genomic integration of the transgene in the mouse lungs was confirmed by PCR. Seven out of the fifteen treated mice showed integration at the mpsL1 site, a previously described 'hot spot' from liver. CONCLUSIONS: These results provide evidence for the activity of phiC31 integrase in lungs but also emphasize the need for optimization of the system to maintain long-term gene expression at high levels. 相似文献
36.
Chae K Isaacs CG Reeves PH Maloney GS Muday GK Nagpal P Reed JW 《The Plant journal : for cell and molecular biology》2012,71(4):684-697
Auxin regulates plant growth and development in part by activating gene expression. Arabidopsis thaliana SMALL AUXIN UP RNAs (SAURs) are a family of early auxin-responsive genes with unknown functionality. Here, we show that transgenic plant lines expressing artificial microRNA constructs (aMIR-SAUR-A or -B) that target a SAUR subfamily (SAUR61-SAUR68 and SAUR75) had slightly reduced hypocotyl and stamen filament elongation. In contrast, transgenic plants expressing SAUR63:GFP or SAUR63:GUS fusions had long hypocotyls, petals and stamen filaments, suggesting that these protein fusions caused a gain of function. SAUR63:GFP and SAUR63:GUS seedlings also accumulated a higher level of basipetally transported auxin in the hypocotyl than did wild-type seedlings, and had wavy hypocotyls and twisted inflorescence stems. Mutations in auxin efflux carriers could partially suppress some SAUR63:GUS phenotypes. In contrast, SAUR63:HA plants had wild-type elongation and auxin transport. SAUR63:GFP protein had a longer half-life than SAUR63:HA. Fluorescence imaging and microsomal fractionation studies revealed that SAUR63:GFP was localized mainly in the plasma membrane, whereas SAUR63:HA was present in both soluble and membrane fractions. Low light conditions increased SAUR63:HA protein turnover rate. These results indicate that membrane-associated Arabidopsis SAUR63 promotes auxin-stimulated organ elongation. 相似文献
37.
Shiou SR Datta PK Dhawan P Law BK Yingling JM Dixon DA Beauchamp RD 《The Journal of biological chemistry》2006,281(45):33971-33981
Metastasis is a primary cause of mortality due to cancer. Early metastatic growth involves both a remodeling of the extracellular matrix surrounding tumors and invasion of tumors across the basement membrane. Up-regulation of extracellular matrix degrading proteases such as urokinase plasminogen activator (uPA) and matrix metalloproteinases has been reported to facilitate tumor cell invasion. Autocrine transforming growth factor-beta (TGF-beta) signaling may play an important role in cancer cell invasion and metastasis; however, the underlying mechanisms remain unclear. In the present study, we report that autocrine TGF-beta supports cancer cell invasion by maintaining uPA levels through protein secretion. Interestingly, treatment of paracrine/exogenous TGF-beta at higher concentrations than autocrine TGF-beta further enhanced uPA expression and cell invasion. The enhanced uPA expression by exogenous TGF-beta is a result of increased uPA mRNA expression due to RNA stabilization. We observed that both autocrine and paracrine TGF-beta-mediated regulation of uPA levels was lost upon depletion of Smad4 protein by RNA interference. Thus, through the Smad pathway, autocrine TGF-beta maintains uPA expression through facilitated protein secretion, thereby supporting tumor cell invasiveness, whereas exogenous TGF-beta further enhances uPA expression through mRNA stabilization leading to even greater invasiveness of the cancer cells. 相似文献
38.
Reversal of an aluminium induced alteration in redox status in different regions of rat brain by administration of centrophenoxine 总被引:5,自引:0,他引:5
Aluminium is one of the most studied neurotoxin, and its effects on nervous system are both structural and functional, involving
various regions of brain. Aluminium toxicity is known to have multiple mechanisms of action in the central nervous system.
Affinity of aluminium for thiol substrates is considered a possible molecular mechanism involved in aluminium neurotoxicity.
The reduced glutathione (GSH) is especially important for cellular defence against aluminium toxicity. This study pertains
to the modulatory action of Centrophenoxine on GSH status in aluminium exposed different brain regions of the female rats.
Aluminium was administered orally at a dose of 40 mg/Kg. b.wt. /day for a period of eight weeks whereas, Centrophenoxine was
administered intraperitoneally at a dose of 100 mg/Kg b.wt./day for a period of six weeks. The study was carried out in different
regions of brain namely Cerebrum, Cerebellum, Medulla Oblongata and Hypothalamus. Animals exposed to aluminum, registered
a significant decrease in the levels of reduced glutathione, and oxidized glutathione as well as in the activity of glutathione
reductase in all the different regions studied when compared to normal control animals. Post-treatment with Centrophenoxine,
showed a significant improvement in the thiol levels in different regions. Centrophenoxine when administered alone also had
a profound effect on the levels of reduced glutathione as well as on the activity of glutathione reductase. From the present
results, it can be stated that Centrophenoxine administration, as a thiol-antioxidant, arrests the aluminium induced cellular
damage by improving the thiol status in brain regions. 相似文献
39.
This study was carried out to investigate the effects of lithium (Li) supplementation on aluminium (Al) induced changes in
antioxidant defence system and histoarchitecture of cerebrum and cerebellum in rats. Al was administered in the form of aluminium
chloride (100 mg/kg b.wt./day, orally) and Li was given in the form of Li carbonate through diet (1.1 g/kg diet, daily) for
a period of 2 months. Al treatment significantly enhanced the levels of lipid peroxidation and reactive oxygen species in
both the cerebrum and cerebellum, which however were decreased following Li supplementation. The enzyme activities of catalase,
superoxide dismutase (SOD) and glutathione reductase (GR) were significantly increased in both the regions following Al treatment.
Li administration to Al-fed rats decreased the SOD, catalase and GR enzyme activities in both the regions; however, in cerebellum
the enzyme activities were decreased in comparison to normal controls also. Further, the specific activity of glutathione-s-transferase and the levels of total and oxidized glutathione were significantly decreased in cerebrum and cerebellum following
Al treatment, which however showed elevation upon Li supplementation. The levels of reduced glutathione were significantly
decreased in cerebrum but increased in cerebellum following Al treatment, which however were normalized upon Li supplementation
but in cerebellum only. Apart from the biochemical changes, disorganization in the layers of cerebrum and vacuolar spaces
were also observed following Al treatment indicating the structural damage. Similarly, the loss of purkinje cells was also
evident in cerebellum. Li supplementation resulted in an appreciable improvement in the histoarchitecture of both the regions.
Therefore, the study shows that Li has a potential to exhibit neuroprotective role in conditions of Al-induced oxidative stress
and be explored further to be treated as a promising drug against neurotoxicity. 相似文献
40.
The present study was aimed to explore the potential of an antidepressant drug lithium (Li) in reducing aluminium (Al) induced
neurotoxicity. To carry out the investigations, Al was administered orally (100 mg AlCl3/Kg b wt/day) whereas, Li was administered through diet (1.1 g Li2CO3/Kg diet, daily) for a total duration of 2 months. Al treatment resulted in a significant increase in the activity of enzyme
nitric oxide synthase and the levels of l-citrulline which, however, were decreased appreciably following lithium supplementation. Al treatment also revealed an increase
in DNA fragmentation as evidenced by an increase in number of comets. Interestingly, Li supplementation to Al treated rats
reduced the damage inflicted on DNA by Al. Ultrastructural studies revealed an increase in chromatin condensation with discontinuity
in nuclear membrane in both the cerebrum and cerebellum of Al treated rats which showed improvement following Li supplementation.
Alterations in the structure of synapse and mitochondrial swelling were also seen. The present study shows the potential of
Li in containing the damage inflicted by Al on rat brain. 相似文献