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741.
Pulp and paper mill effluent was decolorized by growth ofAspergillus niger. Adding glucose (2.0 g/l) and NH4H2PO4 (1.0 g/l) improved decolorization by the fungus (leaving 19% of original colour) and reduced the BOD5 (43%) and the COD (41%) of the effluent after 48 h of incubation.
This work was carried out at the Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore-641 003, India. 相似文献
Résumé L'effluent d'un atelier de pâte à papier a été décoloré par la croissance d'Aspergillus niger. L'ajout de glucose (2.0 g/l) et de NH4H2PO4 (1.0 g/l) a amélioré la décoloration de l'effluent par la molsissure, lalssant 19% de la couleur originale, réduisant la DBO5 de 43% et la DCO de 41%, après 48 h d'incubation.
This work was carried out at the Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore-641 003, India. 相似文献
742.
743.
In this paper we consider the competing risks model where the risks may not be independent. We assume both fixed and random censoring. The random censoring mechanism could have either a parametric or a non-parametric form. The life distributions and the parametric censoring distribution considered are exponential or Weibull. The expressions for the asymptotic confidence intervals for various parameters of interest under different models, using the estimated Fisher information matrix and parametric bootstrap techniques have been derived. Monte Carlo simulation studies for some of these cases have been carried out. 相似文献
744.
Length-weight relationship of six coastal fish species from Gulf of Mannar,South-east coast of India
Sivadas Madhavan Suresh Kumar Karuppiah Kannan Karuppiah 《Zeitschrift fur angewandte Ichthyologie》2020,36(3):364-365
The parameters ‘a’ and ‘b’ of length weight relationship of 6 species (Scolopsis vosmeri (Bloch, 1792), Engyprosopon macrolepis (Regan, 1908), Bleekeria murtii Joshi, Zacharia&Kanthan, 2012), Centriscus scutatus Linnaeus, 1758, Samaris cristatus Gray, 1831 and Iniistius bimaculatus Ruppell, 1829) are reported from the Gulf of Mannar, South east coast of India. The fish samples were collected from experimental trawling conducted twice a month during the period August to December 2012 and September to December 2013 at a depth ranging from 8.5 to 20.5 m. Three hauls were made in a day with each haul of one hour duration. The total length of the gear was 32 m with a cod end mesh size of 30 mm. The total length of the fish was measured to the nearest 0.1 cm and weight to the nearest 0.1 g. 相似文献
745.
746.
The various physico-chemical factors such as rainfall, depth, water spread, temperature, dissolved oxygen, pH, redox potential, alkalinity, total phosphorus, nitrate, kjeldahl nitrogen and dissolved organic matter were studied both diurnally and seasonally and depth-wise for the period from April 1977 to March 1978. Consideration of physico-chemical factors indicates that they are inter-related. The main characteristics of Sathiar clearly indicate that it is highly eutrophic. 相似文献
747.
Kannan Sivakumar Valliappan Karuppiah Gunasekaran Vijayabaskara Sethubathi Thirunavukarasu Thangaradjou Lakshmanan Kannan 《Biologia》2012,67(1):32-40
Amylases constitute one of the most important groups of enzymes for commercial use. In the present study, production of α-amylase was optimized using a newly isolated actinobacterial strain from the coral reef environment of the Gulf of Mannar
Biosphere Reserve, India. It was identified as Streptomyces sp. ML12 based on chemotaxonomy, cultural and morphological characteristics, carbon source utilization and 16S rRNA gene
sequencing. Fermentation variables were selected in accordance with the Plackett-Burman design and were optimized by response
surface methodology. Five significant variables (rice bran and wheat bran — both agricultural byproducts, sodium chloride,
magnesium sulphate and incubation period) were selected for the optimization via central composite design. The optimal features
were rice bran (5.5 g/100 mL), wheat bran (5.3 g/100 mL), sodium chloride (2.8 g/100 mL), magnesium sulphate (1.4 g/100 mL)
and 8 days of incubation period. Optimization of the medium with the above tested features increased the amylase yield by
4.4-fold. 相似文献
748.
The presynaptic neutrotoxin-phospholipase, Notechis II-5 from the venom of NotechisScutatus scutatus (Australian tiger snake) has been crystallized in a form suited for x-ray diffraction analysis. The crystals belong to the
orthorhombic space group P21 21,21, with unit cell dimensions, a=146.1,b =43.5 and c =39.0 A. There are two molecules of Notechis II-5 in the asymmetric unit. The molecular weight is about 13,500.
Notechis II-5 is highly homologous to Notexin, another presynaptic toxin from the venom of the Australian tiger snake, to
bovine and porcine pancreatic phospholipases A and other venom phospholipases. 相似文献
749.
Thirumalai R. Kannan Manickam Krishnan Kumaraguruparan Ramasamy Argentina Becker Olga N. Pakhomova P. John Hart Joel B. Baseman 《Molecular microbiology》2014,93(3):568-581
Community‐acquired respiratory distress syndrome (CARDS) toxin from Mycoplasma pneumoniae is a 591‐amino‐acid virulence factor with ADP‐ribosyltransferase (ADPRT) and vacuolating activities. It is expressed at low levels during in vitro growth and at high levels during colonization of the lung. Exposure of experimental animals to purified recombinant CARDS toxin alone is sufficient to recapitulate the cytopathology and inflammatory responses associated with M. pneumoniae infection in humans and animals. Here, by molecular modelling, serial truncations and site‐directed mutagenesis, we show that the N‐terminal region is essential for ADP‐ribosylating activity. Also, by systematic truncation and limited proteolysis experiments we identified a portion of the C‐terminal region that mediates toxin binding to mammalian cell surfaces and subsequent internalization. In addition, the C‐terminal region alone induces vacuolization in a manner similar to full‐length toxin. Together, these data suggest that CARDS toxin has a unique architecture with functionally separable N‐terminal and C‐terminal domains. 相似文献
750.
Ram Kannan Aravind Mittur Yuzhou Bao Takashi Tsuruo Neil Kaplowitz 《Journal of neurochemistry》1999,73(1):390-399
We have previously shown GSH transport across the blood-brain barrier in vivo and expression of transport in Xenopus laevis oocytes injected with bovine brain capillary mRNA. In the present study, we have used MBEC-4, an immortalized mouse brain endothelial cell line, to establish the presence of Na+-dependent and Na+-independent GSH transport and have localized the Na+-dependent transporter using domain-enriched plasma membrane vesicles. In cells depleted of GSH with buthionine sulfoximine, a significant increase of intracellular GSH could be demonstrated only in the presence of Na+. Partial but significant Na+ dependency of [35S]GSH uptake was observed for two GSH concentrations in MBEC-4 cells in which gamma-glutamyltranspeptidase and gamma-glutamylcysteine synthetase were inhibited to ensure absence of breakdown and resynthesis of GSH. Uniqueness of Na+-dependent uptake in MBEC-4 cells was confirmed with parallel uptake studies with Cos-7 cells that did not show this activity. Molecular form of uptake was verified as predominantly GSH, and very little conversion of [35S]cysteine to GSH occurred under the same incubation conditions. Poly(A)+ RNA from MBEC expressed GSH uptake with significant (approximately 40-70%) Na+ dependency, whereas uptake expressed by poly(A)+ RNA from HepG2 and Cos-1 cells was Na+ independent. Plasma membrane vesicles from MBEC were separated into three fractions (30, 34, and 38% sucrose, by wt) by density gradient centrifugation. Na+-dependent glucose transport, reported to be localized to the abluminal membrane, was found to be associated with the 38% fraction (abluminal). Na+-dependent GSH transport was present in the 30% fraction, which was identified as the apical (luminal) membrane by localization of P-glycoprotein 170 by western blot analysis. Localization of Na+-dependent GSH transport to the luminal membrane and its ability to drive up intracellular GSH may find application in the delivery of supplemented GSH to the brain in vivo. 相似文献