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141.
记内蒙古Juxia一新种 总被引:1,自引:1,他引:0
本文记述了在内蒙古沙拉木伦额尔登敖包地区第三系下渐新统乌兰戈楚组中发现的始巨犀的一个新种:寿氏始巨犀(Juxia shoui)。据其前臼齿及鼻切迹的位置等特点,这一新种当为始巨犀属中比较进步的一个成员,是包氏始巨犀和巨犀之间的过渡类型的犀类动物。 相似文献
142.
青海藏族青少年骨龄与生长发育关系研究 总被引:1,自引:0,他引:1
本文报告了青海省境内,世居在海拔3000-4000米地区的728名7-18岁健康藏族青少年学生的手、腕部骨骼发育情况,对骨化中心出现和骨骺愈合求出了50%出现年龄,并对骨龄与青春期身高突增的关系及与月经初衬潮的关系进行了分析。 相似文献
143.
云南景颇族的体质特征 总被引:17,自引:10,他引:7
本文调查了云南景颇族261人(男105人,女156人)。年龄为成年人(男24-60岁,女23-55岁)。主要测量均值与现代中国汉族和云南省各少数民族比较和聚类分析的结果表明,景颇族属黄种人的东亚类型,但也有南亚类型特征表现。体质特征与傣族、哈尼族和彝族接近,与基诺族和布郎族较远。 相似文献
144.
The biosynthesis of caldariellaquionone (CQ) was studied in species of Sulfolobus by measuring the incorporation of stable isotopically labeled tyrosines into CQ. By feeding a series of tyrosines labeled with deuterium or 13C and then measuring the extent and position at which label was incorporated into CQ by mass spectrometry, it was shown that more than 95% of the label was incorporated into the benzo[b]thiophen-4,7-quinone moiety of CQ. From the labeling experiments, it is concluded that the benzo[b]thiophen-4,7-quinone is derived as an intact unit from all of the carbons of tyrosine except C-1. 相似文献
145.
J Z Wang C V Rojas J H Zhou L S Schwartz H Nicholas E P Hoffman 《Biochemical and biophysical research communications》1992,182(2):794-801
The amino acid sequence of the sodium channel alpha subunit from adult human skeletal muscle has been deduced by cross-species PCR-mediated cloning and sequencing of the cDNA. The protein consists of 1836 amino acid residues. The amino acid sequence shows 93% identity to the alpha subunit from rat adult skeletal muscle and 70% identity to the alpha subunit from other mammalian tissues. A 500 kb YAC clone containing the complete coding sequence and two overlapping lambda clones covering 68% of the cDNA were used to estimate the gene size at 35 kb. The YAC clone proved crucial for gene structure studies as the high conservation between ion channel genes made hybridization studies with total genomic DNA difficult. Our results provide valuable information for the study of periodic paralysis and paramyotonia congenita, two inherited neurological disorders which are caused by point mutations within this gene. 相似文献
146.
147.
M Favrot R Capdeville V Combaret D C Zhou G Clapisson J Banchereau C R Franks S Chouaib J Y Blay T Philip 《European cytokine network》1990,1(3):141-147
The effect of human IL-4, used as a single agent or in combination with low or high dose IL-2, upon LAK-cell proliferation and activation has been tested on PBMC from patients treated with alpha 2-IFN and IL-2. Four days in vitro culture with IL-4 did not induce any LAK-cell activation; IL-4 induced the proliferation of CD3+ CD4+ T-cells, but decreased the percentage of NK cells in culture samples. When combined with high dose IL-2, IL-4 improved the recovery of MN cell without modification of T-cell subsets; however, IL-4 had no major effect on IL-2-induced NK or LAK cell activity. The combination of IL-4 and low dose IL-2 still significantly improved the total MN cell recovery but did not modify the distribution of T and NK lymphocytes; IL-4 inhibited low dose IL-2-induced NK and LAK cell activity, and increased the BL-esterase activity induced by high or low dose IL-2. The combination of IL-4 and IL-2 did not induce any large variation in the percentage of IL-2R (p55) expressing cells. In all tested conditions, IL-2R (p55) was mainly expressed on CD4+ T cells; less than 2% of the cells coexpressed the NK cell marker CD56 and IL-2R (p55). The effect of IL-4 upon IL-2-induced LAK cell expansion is thus very different on PBMC pre-activated in vivo by alpha IFN + IL-2 therapy than on PBMC pre-treated in vitro with IL-2.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
148.
Modulation of ζ-Protein Kinase C by Cyclic AMP in PC12 Cells Occurs Through Phosphorylation by Protein Kinase A 总被引:1,自引:1,他引:0
Marie W. Wooten M. Lamar Seibenhener Laura H. Matthews Guisheng Zhou Elaine S. Coleman 《Journal of neurochemistry》1996,67(3):1023-1031
Abstract: Although cyclic AMP (cAMP) has been reported to cross talk with the protein kinase C (PKC) system, effects of elevated intracellular cAMP on the activities of specific PKC isoforms have not been studied. We report findings from a permeabilized cell assay that was used to examine changes in the activity of the atypical PKC isoforms brought about by exposure of PC12 cells to agents that elevate intracellular cAMP. We found that increases in intracellular cAMP led to rapid stimulation of atypical PKC activity, 40–70% above control, for a sustained period of time, a response that occurred independent of the phorbol 12-myristate 13-acetate (PMA)-sensitive PKC isoforms. Changes in intracellular cAMP levels resulted in a dose-dependent redistribution of ζ-PKC to the cytoplasm with a concomitant increase in the phosphorylation state of the enzyme. Incubation of purified ζ-PKC with increasing concentrations of PKA likewise caused a twofold increase in the phosphorylation state of ζ-PKC. In contrast to the positive effect that PKA-mediated phosphorylation had on the activity of ζ-PKC, the enzyme displayed reduced binding to ras when phosphorylated. Taken together, these findings are consistent with the hypothesis that protein phosphorylation of PKC acts as a positive effector of its enzyme activity and may serve as a negative modulator for interaction with other proteins. 相似文献
149.
Heterogeneity of the glutathione transferase genes encoding enzymes responsible for insecticide degradation in the housefly 总被引:6,自引:0,他引:6
Michael Syvanen Zonghan Zhou Jonathan Wharton Claire Goldsbury Alan Clark 《Journal of molecular evolution》1996,43(3):236-240
One of the four glutathione-S-transferases (GST) that is overproduced in the insecticide-resistant Cornell-R strain of the housefly (Musca domestica) produces an activity that degrades the insecticide dimethyl parathion and conjugates glutathione to lindane. In earlier
work, it was shown that the resistant Cornell-R carries an amplification, probably a duplication, of one or more of its GST
loci and that this amplification is directly related to resistance. Using polymerase chain reaction (PCR) amplification with
genomic DNA, multiple copies of the gene encoding the parathion-degrading activity (called MdGst-3) were subcloned from both
the ancestral, insecticide-susceptible strain BPM and from the insecticide-resistant Cornell-R. In BPM, three different MdGst-3
genes were identified while in Cornell-R, 12 different MdGst-3 sequences were found that, though closely related to ancestral
genes, had diverged by a few nucleotides. This diversity in MdGst-3 genomic sequences in Cornell-R is reflected in the expressed
sequences, as sampled through a cDNA bank. Population heterozygosity cannot account for these multiple GST genes. We suggest
that selection for resistance to insecticides has resulted in not only amplification of the MdGst-3 genes but also in the
divergence of sequence between the amplified copies.
Received: 22 November 1995 / Accepted: 23 February 1996 相似文献
150.
Identification and Characterization of Autosomal Genes That Interact with Glass in the Developing Drosophila Eye
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The glass gene encodes a zinc finger, DNA-binding protein that is required for photoreceptor cell development in Drosophila melanogaster. In the developing compound eye, glass function is regulated at two points: (1) the protein is expressed in all cells' nuclei posterior to the morphogenetic furrow and (2) the ability of the Glass protein to regulate downstream genes is largely limited to the developing photoreceptor cells. We conducted a series of genetic screens for autosomal dominant second-site modifiers of the weak allele glass(3), to discover genes with products that may regulate glass function at either of these levels. Seventy-six dominant enhancer mutations were recovered (and no dominant suppressors). Most of these dominant mutations are in essential genes and are associated with recessive lethality. We have assigned these mutations to 23 complementation groups that include multiple alleles of Star and hedgehog as well as single alleles of Delta, roughened eye, glass and hairy. Mutations in 18 of the complementation groups are embryonic lethals, and of these, 13 show abnormal adult retinal phenotypes in homozygous clones, usually with altered numbers of photoreceptor cells in some of the ommatidia. 相似文献