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181.
The yeast transcription elongation factor Spt4/5 is a sequence‐specific RNA binding protein
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182.
Malgorzata Kotulska Joanna Basalyga Maria B. Derylo Przemyslaw Sadowski 《The Journal of membrane biology》2010,236(1):37-41
Single metastable nanopores, appearing before the actual electroporation under constant-current conditions, are used to characterize
the onset of electroporation. Unlike the long-lived electropores typical of the current controlled methods, these pores survive
for milliseconds and observing them is possible due to slow development of electroporation, provided by the gradual accumulation
of charges on a planar membrane. Analysis of the metastable pore appearance frequency and lifetime shows the first introductory
stage of electroporation. During this stage two species of metastable pores open, the majority of very low conductance that
seem not fully developed as hydrophilic electropores. The experiments reveal that voltage value defines the electroporation
onset while the current value affects the rate of electroporation. Membrane capacitance has a great impact on the membrane
susceptibility to the pore appearance, related to its thickness and integrity. Pores of nonperfect membranes appear more easily,
but they do not live any longer than others. 相似文献
183.
Iva Machová Jan Sná?el Michael Zimmermann Daniel Laubitz Przemyslaw Plocinski Wulf Oehlmann Mahavir Singh Ji?í Dostál Uwe Sauer Iva Pichová 《The Journal of biological chemistry》2014,289(19):13066-13078
Tuberculosis remains a major health concern worldwide. Eradication of its causative agent, the bacterial pathogen Mycobacterium tuberculosis, is particularly challenging due to a vast reservoir of latent carriers of the disease. Despite the misleading terminology of a so-called dormant state associated with latent infections, the bacteria have to maintain basic metabolic activities. Hypoxic conditions have been widely used as an in vitro system to study this dormancy. Such studies identified a rearrangement of central carbon metabolism to exploit fermentative processes caused by the lack of oxygen. Phosphoenolpyruvate carboxykinase (Pck; EC 4.1.1.32) is the enzyme at the center of these metabolic rearrangements. Although Pck is associated with gluconeogenesis under standard growth conditions, the enzyme can catalyze the reverse reaction, supporting anaplerosis of the tricarboxylic acid cycle, under conditions leading to slowed or stopped bacterial replication. To study the mechanisms that regulate the switch between two Pck functions, we systematically investigated factors influencing the gluconeogenic and anaplerotic reaction kinetics. We demonstrate that a reducing environment, as found under hypoxia-triggered non-replicating conditions, accelerates the reaction in the anaplerotic direction. Furthermore, we identified proteins that interact with Pck. The interaction between Pck and the reduced form of mycobacterial thioredoxin, gene expression of which is increased under hypoxic conditions, also increased the Pck anaplerotic activity. We thus propose that a reducing environment and the protein-protein interaction with thioredoxin in particular enable the Pck anaplerotic function under fermentative growth conditions. 相似文献
184.
Shinya Ito Fang-Chin Yeh Emma Hiolski Przemyslaw Rydygier Deborah E. Gunning Pawel Hottowy Nicholas Timme Alan M. Litke John M. Beggs 《PloS one》2014,9(8)
Understanding the detailed circuitry of functioning neuronal networks is one of the major goals of neuroscience. Recent improvements in neuronal recording techniques have made it possible to record the spiking activity from hundreds of neurons simultaneously with sub-millisecond temporal resolution. Here we used a 512-channel multielectrode array system to record the activity from hundreds of neurons in organotypic cultures of cortico-hippocampal brain slices from mice. To probe the network structure, we employed a wavelet transform of the cross-correlogram to categorize the functional connectivity in different frequency ranges. With this method we directly compare, for the first time, in any preparation, the neuronal network structures of cortex and hippocampus, on the scale of hundreds of neurons, with sub-millisecond time resolution. Among the three frequency ranges that we investigated, the lower two frequency ranges (gamma (30–80 Hz) and beta (12–30 Hz) range) showed similar network structure between cortex and hippocampus, but there were many significant differences between these structures in the high frequency range (100–1000 Hz). The high frequency networks in cortex showed short tailed degree-distributions, shorter decay length of connectivity density, smaller clustering coefficients, and positive assortativity. Our results suggest that our method can characterize frequency dependent differences of network architecture from different brain regions. Crucially, because these differences between brain regions require millisecond temporal scales to be observed and characterized, these results underscore the importance of high temporal resolution recordings for the understanding of functional networks in neuronal systems. 相似文献
185.
Devin L. O'Connor Adam Runions Aaron Sluis Jennifer Bragg John P. Vogel Przemyslaw Prusinkiewicz Sarah Hake 《PLoS computational biology》2014,10(1)
The hormone auxin plays a crucial role in plant morphogenesis. In the shoot apical meristem, the PIN-FORMED1 (PIN1) efflux carrier concentrates auxin into local maxima in the epidermis, which position incipient leaf or floral primordia. From these maxima, PIN1 transports auxin into internal tissues along emergent paths that pattern leaf and stem vasculature. In Arabidopsis thaliana, these functions are attributed to a single PIN1 protein. Using phylogenetic and gene synteny analysis we identified an angiosperm PIN clade sister to PIN1, here termed Sister-of-PIN1 (SoPIN1), which is present in all sampled angiosperms except for Brassicaceae, including Arabidopsis. Additionally, we identified a conserved duplication of PIN1 in the grasses: PIN1a and PIN1b. In Brachypodium distachyon, SoPIN1 is highly expressed in the epidermis and is consistently polarized toward regions of high expression of the DR5 auxin-signaling reporter, which suggests that SoPIN1 functions in the localization of new primordia. In contrast, PIN1a and PIN1b are highly expressed in internal tissues, suggesting a role in vascular patterning. PIN1b is expressed in broad regions spanning the space between new primordia and previously formed vasculature, suggesting a role in connecting new organs to auxin sinks in the older tissues. Within these regions, PIN1a forms narrow canals that likely pattern future veins. Using a computer model, we reproduced the observed spatio-temporal expression and localization patterns of these proteins by assuming that SoPIN1 is polarized up the auxin gradient, and PIN1a and PIN1b are polarized to different degrees with the auxin flux. Our results suggest that examination and modeling of PIN dynamics in plants outside of Brassicaceae will offer insights into auxin-driven patterning obscured by the loss of the SoPIN1 clade in Brassicaceae. 相似文献
186.
Slawomir Ciesielski Katarzyna Bułkowska Dorota Dabrowska Dariusz Kaczmarczyk Przemyslaw Kowal Justyna Możejko 《Current microbiology》2013,67(2):240-248
The applicability of a newly-designed PCR primer pair in examination of methanogenic Archaea in a digester treating plant biomass was evaluated by Ribosmal Intergenic Spacer Analysis (RISA). To find a suitable approach, three variants of RISA were tested: (1) standard, polyacrylamide gel-based, (2) automated, utilized capillary electrophoresis (GA-ARISA), and (3) automated microfluidics-based (MF-ARISA). All three techniques yielded a consistent picture of archaeal community structure changes during anaerobic digestion monitored for more than 6 weeks. While automated variants were more practical for handling and rapid analysis of methanogenic Archaea, the gel-based technique was advantageous when micro-organism identification was required. A DNA-sequence analysis of dominant bands extracted from the gel revealed that the main role in methane synthesis was played by micro-organisms affiliated with Methanosarcina barkeri. The obtained results revealed that RISA is a robust method allowing for detailed analysis of archaeal community structure during organic biomass conversion into biogas. In addition, our results showed that GA-ARISA has a higher resolution and reproducibility than other variants of RISA and could be used as a technique for tracking changes in methanogenic Archaea in an anaerobic digester. 相似文献
187.
Jakub Piotr Fichna Justyna Karolczak Anna Potulska-Chromik Przemyslaw Miszta Mariusz Berdynski Agata Sikorska Slawomir Filipek Maria Jolanta Redowicz Anna Kaminska Cezary Zekanowski 《PloS one》2014,9(12)
Desmin is a muscle-specific intermediate filament protein which forms a network connecting the sarcomere, T tubules, sarcolemma, nuclear membrane, mitochondria and other organelles. Mutations in the gene coding for desmin (DES) cause skeletal myopathies often combined with cardiomyopathy, or isolated cardiomyopathies. The molecular pathomechanisms of the disease remain ambiguous. Here, we describe and comprehensively characterize two DES mutations found in Polish patients with a clinical diagnosis of desminopathy. The study group comprised 16 individuals representing three families. Two mutations were identified: a novel missense mutation (Q348P) and a small deletion of nine nucleotides (A357_E359del), previously described by us in the Polish population. A common ancestry of all the families bearing the A357_E359del mutation was confirmed. Both mutations were predicted to be pathogenic using a bioinformatics approach, including molecular dynamics simulations which helped to rationalize abnormal behavior at molecular level. To test the impact of the mutations on DES expression and the intracellular distribution of desmin muscle biopsies were investigated. Elevated desmin levels as well as its atypical localization in muscle fibers were observed. Additional staining for M-cadherin, α-actinin, and myosin heavy chains confirmed severe disruption of myofibrill organization. The abnormalities were more prominent in the Q348P muscle, where both small atrophic fibers as well large fibers with centrally localized nuclei were observed. We propose that the mutations affect desmin structure and cause its aberrant folding and subsequent aggregation, triggering disruption of myofibrils organization. 相似文献
188.
189.
Partial migration to seasonally‐unstable habitat facilitates biological invasions in a predator‐dominated system
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Przemyslaw G. Bajer James E. Parker Timothy K. Cross Paul A. Venturelli Peter W. Sorensen 《Oikos》2015,124(11):1520-1526
Although partial migration, a phenomenon in which some individuals in a population conduct seasonal migrations while others remain resident, is common among animals, its importance in facilitating biological invasions has not been demonstrated. To illustrate how partial migration might facilitate invasions in spatially complex habitats, we developed an individual‐based model of common carp Cyprinus carpio in systems of lakes and winterkill‐prone marshes in the Upper Mississippi River Basin (UMRB). Our model predicted that common carp are unable to become invasive in lakes of the UMRB unless they conduct partial migrations into winterkill‐prone marshes in which recruitment rates are high in the absence of native predators that forage on carp eggs and larvae. Despite low dispersal rates of juveniles and higher mortality rates of migrants, partial migration was adaptive across a wide range of migration rates and winterkill frequencies. Partial migration rates as low as 10% and winterkill occurrence as infrequent as once in 20 years were sufficient to cause invasiveness because of carp's reproductive potential and longevity. Consistent with the results of our model, empirical data showed that lake connectivity to winterkill‐prone marshes was an important driver of carp abundance within the study region. Our results demonstrate that biological invasions may be driven by a small, migratory contingent of a population that exploits more beneficial reproductive habitats. 相似文献
190.
Liver disturbances stimulate inflammatory reaction in the brain but little is known if injury to the brain can significantly influence liver metabolism. This problem is crucial in modern transplantology, as the condition of the donor brain seems to strongly affect the quality (viability) of the graft, which is often obtained from brain-dead donors, usually after traumatic brain injury. Because nitric oxide is one of the significant molecules in brain and liver biology, we examined if brain injury can affect NO level in the liver. Liver samples of Wistar rats were collected and studied with EPR NO-metry to detect NO level changes at different time points after brain injury. Shortly after the trauma, NO level in the liver was similar to the control. However, later there was a significant increase in the NO content in the livers starting from the 2nd day after brain injury and lasting up to the 7th day. It seems that the response to a mechanical brain injury is of the systemic, rather than local character. Therefore brain metabolism disturbances can influence liver metabolism at least by stimulating the organ to produce NO. 相似文献