全文获取类型
收费全文 | 2395篇 |
免费 | 226篇 |
出版年
2023年 | 24篇 |
2022年 | 39篇 |
2021年 | 88篇 |
2020年 | 39篇 |
2019年 | 46篇 |
2018年 | 81篇 |
2017年 | 61篇 |
2016年 | 72篇 |
2015年 | 91篇 |
2014年 | 117篇 |
2013年 | 165篇 |
2012年 | 182篇 |
2011年 | 142篇 |
2010年 | 103篇 |
2009年 | 69篇 |
2008年 | 98篇 |
2007年 | 78篇 |
2006年 | 88篇 |
2005年 | 82篇 |
2004年 | 61篇 |
2003年 | 70篇 |
2002年 | 54篇 |
2001年 | 44篇 |
2000年 | 29篇 |
1999年 | 34篇 |
1998年 | 18篇 |
1997年 | 21篇 |
1996年 | 17篇 |
1995年 | 13篇 |
1994年 | 13篇 |
1993年 | 19篇 |
1992年 | 39篇 |
1991年 | 33篇 |
1990年 | 37篇 |
1989年 | 36篇 |
1988年 | 25篇 |
1987年 | 27篇 |
1986年 | 39篇 |
1985年 | 30篇 |
1984年 | 33篇 |
1983年 | 19篇 |
1982年 | 19篇 |
1981年 | 20篇 |
1980年 | 14篇 |
1979年 | 25篇 |
1978年 | 17篇 |
1977年 | 20篇 |
1976年 | 20篇 |
1975年 | 24篇 |
1974年 | 24篇 |
排序方式: 共有2621条查询结果,搜索用时 265 毫秒
31.
Saburo Sone Teruhiro Utsugi Priti Tandon Mitsumasa Ogawara 《Cancer immunology, immunotherapy : CII》1986,22(3):191-196
Summary Studies were performed on the activation of human blood monocytes to the antitumor state by a dried preparation of multilamellar vesicle (MLV) liposomes in which synthetic muramyl tripeptide phosphatidylethanolamine (MTP-PE) was inserted directly into the liposome membrane. Dried liposomes composed of synthetic phospholipids [phosphatidylcholine (PC) and phosphatidylserine (PS) in a molar ratio of 7:3] were prepared by lyophilization. Dried liposome-MTP-PE was found to be superior in several ways to free desmethyl muramyl dipeptide (norMDP) or conventional liposome-MTP-PE, prepared immediately before use. First, dried lipsome-MTP-PE was stable and strongly activated monocytes when stored for over 3 months in a freezer at –°C or even in suspension at 4°C. Second, human monocytes in suspension, as well as in the adherent form, were activated to the tumoricidal state by interaction for at least 4 h with the dried preparation of liposome-MTP-PE. Third, monocytes activated with the dried liposome-MTP-PE or conventionally prepared liposome-MTP-PE maintained their tumoricidal activity for a longer period (4 days) than those activated with free norMDP. These results indicate that the dried preparation of liposome-MTP-PE can be stored for a long time, has a reproducible effect that can be standardized and should be valuable for in situ activation of human monocytes to the tumoricidal state, which is associated with eradication of cancer metastases. 相似文献
32.
33.
34.
Copper uptake by free and immobilized cyanobacterium 总被引:1,自引:0,他引:1
Abstract Copper uptake in free and immobilized cells of the cyanobacterium Nostoc calcicola has been examined. The immobilized cells invariably maintained a higher profile of Cu intake rate (12.7 nmol mg−1 protein min−1 ) over the free cells (6.0 nmol mg−1 protein min−1 ). The total Cu uptake in immobilized cells was almost two and a half-times more than their free cell counterpart under identical experimental conditions. Also, the immobilized cells showed a stronger positive correlation between Cu adsorption and uptake. The results have been discussed in terms of improved metabolic efficiency of immobilized cells. 相似文献
35.
36.
37.
Functional antagonism between oncoprotein c-Jun and the glucocorticoid receptor 总被引:121,自引:0,他引:121
R Schüle P Rangarajan S Kliewer L J Ransone J Bolado N Yang I M Verma R M Evans 《Cell》1990,62(6):1217-1226
38.
Purification and characterization of the inositol 1,4,5-trisphosphate receptor protein from rat vas deferens 总被引:5,自引:0,他引:5
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Robert J. Mourey Ajay Verma Surachai Supattapone Solomon H. Snyder 《The Biochemical journal》1990,272(2):383-389
Among rat peripheral tissues examined, Ins(1,4,5)P(3) receptor binding is highest in the vas deferens, with levels about 25% of those of the cerebellum. We have purified the InsP(3) receptor binding protein from rat vas deferens membranes 600-fold. The purified protein displays a single 260 kDa band on SDS/PAGE, and the native protein has an apparent molecular mass of 1000 kDa, the same as in cerebellum. The inositol phosphate specificity, pH-dependence and influence of various reagents are the same for purified vas deferens and cerebellar receptors. Whereas particulate InsP(3) binding in cerebellum is potently inhibited by Ca(2+), particulate and purified vas deferens receptor binding of InsP(3) is not influenced by Ca(2+). Vas deferens appears to lack calmedin activity, but the InsP(3) receptor is sensitive to Ca(2+) inhibition conferred by brain calmedin. The vas deferens may prove to be a valuable tissue for characterizing functional aspects of InsP(3) receptors. 相似文献
39.
Developmental expression of the Xenopus laevis fos protooncogene 总被引:3,自引:0,他引:3
40.
Rat brain endoplasmic reticulum calcium pools are anatomically and functionally segregated. 总被引:3,自引:1,他引:2
Autoradiographic imaging can localize 45Ca2+ selectively accumulated via the Ca2+, Mg2(+)-ATPase into endoplasmic reticulum stores in rat brain slices. 45Ca2+ accumulation is markedly stimulated by oxalate and displays a heterogeneous distribution which resembles the mRNA distribution for a sarcoendoplasmic reticulum Ca2+, Mg2(+)-ATPase. Inositol 1,4,5-triphosphate [I(1,4,5)P3] inhibits 45Ca2+ accumulation selectively into regions corresponding to those enriched in I(1,4,5)P3 receptor-binding sites and in Ca2+, -Mg2(+)-ATPase mRNA. Thus rat brain endoplasmic reticulum calcium stores are anatomically and functionally differentiated. 相似文献