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51.
P. Dufour J. -M. Lang C. Giron B. Duclos P. Haehnel D. Jaeck J. -M. Jung F. Oberling 《Biotherapy》1993,6(1):9-12
Sixty-four patients with non metastatic high risk breast cancer were randomized in a double blind trial of adjuvant immunotherapy with sodium ditiocarb (DDC) versus placebo. All patients underwent prior surgery (mammectomy according to Patey) then adjuvant FAC chemotherapy +/- DDC. With a median follow-up of 5 years we observed 6 relapses and 5 deaths in DDC group; 13 relapses and 12 deaths in control group. At 6 years, overall survival is 81% in DDC group versus 55%. Disease free survival (DFS) is 76% in DDC group versus 55%. DDC associated to chemotherapy and locoregional treatment can improve survival and probably DFS in this high risk breast cancer subgroup.Abbreviations DDC
sodium ditiocarb
- DFS
disease free survival
- OS
overall survival
- N
node
- FAC
5 Fluorouracil, Adrianycin, Cyclopho-pharmide 相似文献
52.
Summary A new G6PD variant, called G6PD Vientiane, has been discovered in a patient from Laos.The characteristics of this variant are: mild enzyme deficiency (about 50% of the normal activity) in the granulocytes and the red cells, with normal G6PD-related antigen concentration; increased stability; normal Km glucose 6-phosphate and NADP+; increased inhibition constant by NADPH; decreased inhibition by ATP; slightly increased utilization of the substrate analogue; abnormal pH curve, with maximum activity at pH 9.5; slightly reduced starch gel electrophoretic migration. The implications of the molecular stability of a deficient mutant variant are discussed. 相似文献
53.
Replication of diabetogenic and nondiabetogenic variants of encephalomyocarditis (EMC) virus in ICR Swiss mice 总被引:1,自引:0,他引:1
C L Gould M L Trombley N J Bigley K G McMannama D J Giron 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1984,175(4):449-453
The replication of the diabetogenic D (EMC-D) and nondiabetogenic B (EMC-B) variants of encephalomyocarditis virus in various tissues of the murine host was determined. Pancreatic insulin levels were also measured. EMC-D replicated in the spleen, pancreas, heart, lung, and intestines, while EMC-B was limited to the spleen and pancreas. EMC-B interfered with the replication of EMC-D in each of the tissues examined. Insulin levels were initially increased by both viruses. By 4 days postinfection, insulin levels were either normal or undetectable in (EMC-D)-infected animals, but were dramatically elevated in those infected with EMC-B. 相似文献
54.
Maria Fernanda de Souza Costa Filipe Pereira-Dutra Nathalie Deboosere Samuel Jouny Ok-Ryul Song Guilherme Iack Andreia Lamoglia Souza Eric Henrique Roma Vincent Delorme Patricia T. Bozza Priscille Brodin 《Molecular microbiology》2023,119(2):224-236
Tuberculosis remains a global health threat with high morbidity. Dendritic cells (DCs) participate in the acute and chronic inflammatory responses to Mycobacterium tuberculosis (Mtb) by directing the adaptive immune response and are present in lung granulomas. In macrophages, the interaction of lipid droplets (LDs) with mycobacteria-containing phagosomes is central to host-pathogen interactions. However, the data available for DCs are still a matter of debate. Here, we reported that bone marrow-derived DCs (BMDCs) were susceptible to Mtb infection and replication at similar rate to macrophages. Unlike macrophages, the analysis of gene expression showed that Mtb infection induced a delayed increase in lipid droplet-related genes and proinflammatory response. Hence, LD accumulation has been observed by high-content imaging in late periods. Infection of BMDCs with killed H37Rv demonstrated that LD accumulation depends on Mtb viability. Moreover, infection with the attenuated strains H37Ra and Mycobacterium bovis-BCG induced only an early transient increase in LDs, whereas virulent Mtb also induced delayed LD accumulation. In addition, infection with the BCG strain with the reintroduced virulence RD1 locus induced higher LD accumulation and bacterial replication when compared to parental BCG. Collectively, our data suggest that delayed LD accumulation in DCs is dependent on mycobacterial viability and virulence. 相似文献
55.
Lifetime gains of host-feeding in a synovigenic parasitic wasp 总被引:3,自引:0,他引:3
Abstract. Understanding behavioural decisions relative to host use for feeding or reproduction by foraging parasitoids requires not only the study of metabolic pathways followed by nutrients, but also the quantification of lifetime fitness gains of each alternative behaviour. By using a combination of observational and manipulative approaches, the lifetime host‐feeding gains are measured both in terms of fecundity and longevity in the parasitoid Eupelmus vuilletti. Host‐feeding increases both egg production and longevity. The increase in fecundity is mainly determined by the amount of lipids obtained whereas the lifespan extension is mainly determined by carbohydrates. Proteins obtained through host‐feeding have been implicated previously in egg production by parasitoids but protein intake has no effect on fecundity and longevity in E. vuilletti. The amount of nutrients gained through host‐feeding and invested in eggs is variable and changes over the lifetime of the animal. Therefore, lifetime feeding gains are best understood through the construction of dynamical budgets running over the entire lifespan of an insect. 相似文献
56.
Adiponectin, a hormone secreted from adipocytes, has been shown to protect against development of insulin resistance, ischemia–reperfusion
injury, and inflammation. Adiponectin assembles into multiple oligomeric isoforms: trimers, hexamers and several higher molecular
weight (HMW) species. Of these, the HMW species are selectively decreased during the onset of type 2 diabetes. Despite the
critical role of HMW adiponectin in insulin responsiveness, its assembly process is poorly understood. In this report, we
investigated the role of divalent cations in adiponectin assembly. Purified adiponectin 18mers, the largest HMW species, did
not collapse to smaller oligomers after treatment with high concentrations of EDTA. However, treatment with EDTA or another
chelator DTPA inhibited the oligomerization of 18mers from trimers in vitro. Zn2+ specifically increased the formation of 18mers when compared with Cu2+, Mg2+, and Ca2+. Distribution of adiponectin oligomers secreted from zinc chelator TPEN-treated rat adipocytes skewed toward increased proportions
of hexamers and trimers. While we observed presence of zinc in adiponectin purified from calf serum, the role of zinc in disulfide
bonding between oligomers was examined because the process is critical for 18mer assembly. Surprisingly, Zn2+ inhibited disulfide bond formation early in the oligomerization process. We hypothesize that initial decreases in disulfide
formation rates could allow adiponectin subunits to associate before becoming locked in fully oxidized conformations incapable
of further oligomerization. These data demonstrate that zinc stimulates oligomerization of HMW adiponectin and possibly other
disulfide-dependent protein assembly processes. 相似文献
57.
Noémie Calland Marie-Emmanuelle Sahuc Sandrine Belouzard Véronique Pène Pierre Bonnafous Ahmed Atef Mesalam Gaspard Deloison Véronique Descamps Sevser Sahpaz Czeslaw Wychowski Olivier Lambert Priscille Brodin Gilles Duverlie Philip Meuleman Arielle R. Rosenberg Jean Dubuisson Yves Rouillé Karin Séron 《Journal of virology》2015,89(19):10053-10063
58.
Tanya L. Rogers Stephan B. Munch Simon D. Stewart Eric P. Palkovacs Alfredo Giron‐Nava Shin‐ichiro S. Matsuzaki Celia C. Symons 《Ecology letters》2020,23(8):1287-1297
Experiments have revealed much about top‐down and bottom‐up control in ecosystems, but manipulative experiments are limited in spatial and temporal scale. To obtain a more nuanced understanding of trophic control over large scales, we explored long‐term time‐series data from 13 globally distributed lakes and used empirical dynamic modelling to quantify interaction strengths between zooplankton and phytoplankton over time within and across lakes. Across all lakes, top‐down effects were associated with nutrients, switching from negative in mesotrophic lakes to positive in oligotrophic lakes. This result suggests that zooplankton nutrient recycling exceeds grazing pressure in nutrient‐limited systems. Within individual lakes, results were consistent with a ‘seasonal reset’ hypothesis in which top‐down and bottom‐up interactions varied seasonally and were both strongest at the beginning of the growing season. Thus, trophic control is not static, but varies with abiotic conditions – dynamics that only become evident when observing changes over large spatial and temporal scales. 相似文献
59.
Pym AS Brodin P Majlessi L Brosch R Demangel C Williams A Griffiths KE Marchal G Leclerc C Cole ST 《Nature medicine》2003,9(5):533-539
The live tuberculosis vaccines Mycobacterium bovis BCG (bacille Calmette-Guérin) and Mycobacterium microti both lack the potent, secreted T-cell antigens ESAT-6 (6-kDa early secretory antigenic target) and CFP-10 (10-kDa culture filtrate protein). This is a result of independent deletions in the region of deletion-1 (RD1) locus, which is intact in virulent members of the Mycobacterium tuberculosis complex. To increase their immunogenicity and protective capacity, we complemented both vaccines with different constructs containing the esxA and esxB genes, which encode ESAT-6 and CFP-10 respectively, as well as a variable number of flanking genes. Only reintroduction of the complete locus, comprising at least 11 genes, led to full secretion of the antigens and resulted in specific ESAT-6-dependent immune responses; this suggests that the flanking genes encode a secretory apparatus. Mice and guinea pigs vaccinated with the recombinant strain BCG::RD1-2F9 were better protected against challenge with M. tuberculosis, showing less severe pathology and reduced dissemination of the pathogen, as compared with control animals immunized with BCG alone. 相似文献
60.
Cannesan MA Gangneux C Lanoue A Giron D Laval K Hawes M Driouich A Vicré-Gibouin M 《Annals of botany》2011,108(3):459-469