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Spassova Mariana I. Prins Theo W. Folkertsma Rolf T. Klein-Lankhorst René M. Hille Jacques Goldbach Rob W. Prins Marcel 《Molecular breeding : new strategies in plant improvement》2001,7(2):151-161
Tomato spotted wilt virus is an important threat to tomato production worldwide. A single dominant resistance gene locus, Sw5, originating from Lycopersicon peruvianum, has been identified and introgressed in cultivated tomato plants. Here we present the genomic organization of a 35 250 bp fragment of a BAC clone overlapping the Sw5 locus. Two highly homologous (95%) resistance gene candidates were identified within 40 kb of the CT220 marker. The genes, tentatively named Sw5-a and Sw5-b, encode proteins of 1245 and 1246 amino acids, respectively, and are members of the coiled-coil, nucleotide-binding-ARC, leucine-rich repeat group of resistance gene candidates. Promoter and terminator regions of the genes are also highly homologous. Both genes significantly resemble the tomato nematode and aphid resistance gene Mi and, to a lesser extent, Pseudomonas syringae resistance gene Prf. Transformation of Nicotiana tabacum cv. SR1 plants revealed that the Sw5-b gene, but not the Sw5-a gene, is necessary and sufficient for conferring resistance against tomato spotted wilt virus. 相似文献
64.
Giovanny Fagua Fabien L. Condamine Jason J. Dombroskie Bong‐Kyu Byun Jurate De Prins Thomas J. Simonsen Marcos Baez Bryan M.T. Brunet Felix A.H. Sperling 《Systematic Entomology》2019,44(1):19-38
Widely known for pest species that include major modulators of temperate forests, the genus Choristoneura is part of the species‐rich tribe Archipini of leafroller moths (Tortricidae). Delimitation of the genus has remained unresolved because no phylogeny has included species endemic to Africa and studies have often omitted the type species of the genus. Further taxonomic confusion has been generated by the transfer of Archips occidentalis (Walsingham) to Choristoneura, creating a homonym with Choristoneura occidentalis Freeman, an important defoliator of North American forests. To define the limits of the genus, we reconstructed a phylogeny using DNA sequences for mitochondrial cytochrome oxidase subunit I and nuclear ribosomal 28S genes. Our ingroup included 23 Choristoneura species‐level taxa, complemented by a large sample of outgroups comprising 82 species of Archipini and other Tortricidae. We generated a time‐calibrated tree using fossil and secondary calibrations and we inferred biogeographic and diversification processes in Choristoneura. Our analysis recovered the genus as polyphyletic, with Archips occidentalis, Choristoneura simonyi and Choristoneura evanidana excluded from the main clade. Based on the recovered phylogenies and a redefinition, we restrict Choristoneura primarily to species with a northern hemisphere distribution. Our analysis supports A. occidentalis as the sister group of Cacoecimorpha pronubana, C. simonyi as the sister of ‘Xenotemna’ pallorana, and C. evanidana as the sister of Archips purpurana. A new combination is proposed: Archips evanidana comb.n. ; the availability of ‘Xenotemna’ as a valid name is discussed and A. occidentalis is considered as an orphaned name within the Archipini. We found support for a Holarctic origin of Choristoneura about 23 Ma, followed by early divergence in the Palearctic region. The main divergence occurred at 16 Ma, with one clade in the Nearctic and another in the Palearctic. Subsequent cladogenetic events were synchronous and related to herbivorous specialization, with each clade divided into coniferophagous and polyphagous lineages. Their specialization as conifer feeders temporally matched the expansion of boreal forest during the Miocene. 相似文献
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66.
A. van?Reenen F. Gutiérrez-Mejía L.J. van?IJzendoorn M.W.J. Prins 《Biophysical journal》2013,104(5):1073-1080
We report a method to profile the torsional spring properties of proteins as a function of the angle of rotation. The torque is applied by superparamagnetic particles and has been calibrated while taking account of the magnetization dynamics of the particles. We record and compare the torsional profiles of single Protein G-Immunoglobulin G (IgG) and IgG-IgG complexes, sandwiched between a substrate and a superparamagnetic particle, for torques in the range between 0.5 × 103 and 5 × 103 pN·nm. Both molecular systems show torsional stiffening for increasing rotation angle, but the elastic and inelastic torsion stiffnesses are remarkably different. We interpret the results in terms of the structural properties of the molecules. The torsion profiling technique opens new dimensions for research on biomolecular characterization and for research on bio-nanomechanical structure-function relationships. 相似文献
67.
Rembrandt Dijkerman Jeroen Ledeboer Huub J.M. Op den Camp Rudolf A. Prins Chris van der Drift 《Current microbiology》1997,34(2):91-96
The anaerobic fungus Neocallimastix sp. strain L2,
isolated from the feces of a llama, was tested for growth on a range of
soluble and insoluble carbohydrate substrates. The fungus was able to ferment
glucose, cellobiose, fructose, lactose, maltose, sucrose, soluble starch,
inulin, filter paper cellulose, and Avicel. No growth was observed on
arabinose, galactose, mannose, ribose, xylose, sorbitol, pectin, xylan,
glycerol, citrate, soya, and wheat bran. The fermentation products after
growth were hydrogen, formate, acetate, ethanol, and lactate. The
fermentation pattern was dependent on the carbon source. In general, higher
hydrogen production resulted in decreased formation of lactate and ethanol.
Recovery of the fermented carbon in products at the end of growth ranged from
50% to 80%. (Hemi)cellulolytic enzyme activities were affected
by the carbon source. Highest activities were found in filtrates from
cultures grown on cellulose. Growing the fungus on inulin and lactose yielded
the lowest cellulolytic activities. Highest specific activities for
avicelase, endoglucanase, β-glucosidase, and xylanase were obtained with
Avicel as the substrate for growth (0.29, 5.9, 0.57, and 13
IU · mg−1 protein, respectively). Endoglucanase activity
banding patterns after SDS-PAGE were very similar for all substrates. Minor
differences indicated that enzyme activities may in part be the result of
secretion of different sets of isoenzymes.
Received: 10 July 1996 / Accepted: 22 July 1996 相似文献
68.
Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. III. Experiments with 14C-labeled substrates 总被引:31,自引:0,他引:31
An ecological substrate relationship between sulfate-reducing and methane-producing bacteria in mud of Lake Vechten has been studied in experiments using 14C-labeled acetate and lactate as substrates. Fluoroacetate strongly inhibited the formation of 14CO2 from [U-14C]-acetate and β-fluorolactate gave an inhibition of similar magnitude of the breakdown of [U-14C]-l-lactate to 14CO2 thus confirming earlier results on the specific action of these inhibitors. The turnover-rate constant of l-lactate was 2.37 hr-1 and the average l-lactate pool size was 12.2 μg per gram of wet mud, giving a turnover rate of 28.9 μg of lactate/gram of mud per hr. The turnover-rate constant of acetate was 0.35 hr-1 and the average pool size was 5.7 μg per gram of wet mud, giving a rate of disappearance of 1.99 μg of acetate/gram of mud per hr. Estimations of the acetate turnover rate based upon the formation of 14CO2 from [U-14C]-acetate or [1-14C]-acetate yielded figures of the same magnitude (range 0.45 to 1.74). These and other results suggest that only a portion of the lactate dissimilated is turned over through the acetate pool. The ratio of 14CO2/14CH4 produced from [U-14C]-acetate by mud was 1.32; indicating that 0.862 moles of CH4 and 1.138 moles of CO2 are formed per mole of acetate. From the rate of disappearance of acetate (0.027 μmoles/gram wet mud per hr) and the rate of methane production (0.034 μmoles/gram wet mud per hr), it may be concluded that acetate is an important precursor of methanogenesis in mud (approximately 70%). A substrate relationship between the two groups of bacteria is likely since 14CH4 was formed from [U-14C]-l-lactate. 相似文献
69.
Isolation, Culture, and Fermentation Characteristics of Selenomonas ruminantium var. bryanti var. n. from the Rumen of Sheep 下载免费PDF全文
R. A. Prins 《Journal of bacteriology》1971,105(3):820-825
Large forms of Selenomonas sp. were isolated from the sheep rumen on a rumen fluid-glucose-agar medium by using a differential centrifugation technique to purify the inoculum. The cells from the six isolated strains were curved, gram-negative, strictly anaerobic crescents, and rapidly motile by flagella attached to the concave side of the cell. One or more of the volatile fatty acids were essential for growth. None of the strains produced indole or reduced nitrate. All strains grew on fructose, glucose, mannose, cellobiose, maltose, sucrose, and salicin. Fermentation end products from glucose were mainly lactate, acetate, propionate, and formate. Small amounts of succinate were formed. The final pH in a glucose medium ranged between 4.3 and 4.5. On the basis of the sugar fermentation characteristics and the capacity to form hydrogen sulfide from cysteine, it is suggested that one of the strains is a large form of Selenomonas ruminantium. The other five strains are designated S. ruminantium var. bryanti, var. n. 相似文献
70.