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131.
132.
Cleida?A?Oliveira Rong?Nie Kay?Carnes Luiz?R?Franca Gail?S?Prins Philippa?TK?Saunders Rex?A?HessEmail author 《Reproductive biology and endocrinology : RB&E》2003,1(1):75
Background
The antiestrogen ICI 182,780 has been used successfully as an alternative experimental model for the study of estrogen action in the rodent adult male reproductive tract. Although ICI 182,780 causes severe alterations in testicular and efferent ductule morphology and function, the effects on the expression of estrogen and androgen receptors in the male have not been shown. 相似文献133.
Sexual differences in herding behaviour ofAfrican buffalo (Syncerus caffer) werestudied by analysing at the herd levelmitochondrial D-loop hypervariable region I andfourteen autosomal microsatellites. Three herdsfrom Arusha National Park in Tanzania wereanalysed with mtDNA and five herds from KrugerNational Park in South Africa with mtDNA andmicrosatellites. Significant mtDNAdifferentiation was observed among herds inArusha NP (FST = 0.12, based on haplotypefrequencies). Assignment tests withmicrosatellite data from Kruger NP showed thatmost frequent migration between herds is bymales two years. This was confirmed bytests for herd differentiation and analyseswith Lynch and Ritland's relatedness estimator.Within a herd, males younger than two years andfemales showed a higher relationship through acommon father rather than a common mother,indicating that female herd members mate withonly a few dominant males. This in turnsuggests a female:male sex ratio larger than5:1. The migration rate per generation betweenherds was estimated to be 5–2% for femalesand close to 100% for males. Finally, theimplications for the management of buffalopopulations are being discussed. 相似文献
134.
135.
Harald E. L. Prins 《American anthropologist》2001,103(1):263-264
Envisioning Power: Ideologies of Dominance and Crisis. Eric R. Wolf. Berkeley: University of California Press, 1999. 339 pp. 相似文献
136.
Harald E.L. Prins 《American anthropologist》2002,104(2):670-672
Twelve Thousand Years: American Indians in Maine. Bruce J. Bourque. with Steven L. Cox and Ruth H. Whitehead. Lincoln: University of Nebraska Press, 2001.368 pp. 相似文献
137.
Founder effect at PGL1 in hereditary head and neck paraganglioma families from the Netherlands. 下载免费PDF全文
E M van Schothorst J C Jansen E Grooters D E Prins J J Wiersinga A G van der Mey G J van Ommen P Devilee C J Cornelisse 《American journal of human genetics》1998,63(2):468-473
PGL1, a gene responsible for hereditary paragangliomas of the head and neck, recently was mapped to a 2-cM interval on chromosome 11q22-q23, by linkage and haplotype-sharing analysis of a large multibranch Dutch family. We determined the disease-linked haplotype, as defined by 13 markers encompassing a large interval on 11q21-q23, in 10 additional families ascertained from the same geographical locale. Alleles were identical for six contiguous markers, spanning a genetic distance of 6 cM and containing PGL1. Despite this strong indication of a common ancestor, no kinships between the families could be demonstrated through genealogical surveys going back to 1800 a.d. We conclude that a single ancestral mutation is responsible for most, if not all, hereditary paragangliomas, in this region of The Netherlands, and that strong founder effects may exist at the PGL1 locus. 相似文献
138.
Isolation of Typical Marine Bacteria by Dilution Culture: Growth, Maintenance, and Characteristics of Isolates under Laboratory Conditions 总被引:28,自引:18,他引:10 下载免费PDF全文
Frits Schut Egbert J. de Vries Jan C. Gottschal Betsy R. Robertson Wim Harder Rudolf A. Prins Don K. Button 《Applied microbiology》1993,59(7):2150-2160
Marine bacteria in Resurrection Bay near Seward, Alaska, and in the central North Sea off the Dutch coast were cultured in filtered autoclaved seawater following dilution to extinction. The populations present before dilution varied from 0.11 × 109 to 1.07 × 109 cells per liter. The mean cell volume varied between 0.042 and 0.074 μm3, and the mean apparent DNA content of the cells ranged from 2.5 to 4.7 fg of DNA per cell. All three parameters were determined by high-resolution flow cytometry. All 37 strains that were obtained from very high dilutions of Resurrection Bay and North Sea samples represented facultatively oligotrophic bacteria. However, 15 of these isolates were eventually obtained from dilution cultures that could initially be cultured only on very low-nutrient media and that could initially not form visible colonies on any of the agar media tested, indicating that these cultures contained obligately oligotrophic bacteria. It was concluded that the cells in these 15 dilution cultures had adapted to growth under laboratory conditions after several months of nutrient deprivation prior to isolation. From the North Sea experiment, it was concluded that the contribution of facultative oligotrophs and eutrophs to the total population was less than 1% and that while more than half of the population behaved as obligately oligotrophic bacteria upon first cultivation in the dilution culture media, around 50% could not be cultured at all. During one of the Resurrection Bay experiments, 53% of the dilution cultures obtained from samples diluted more than 2.5 × 105 times consisted of such obligate oligotrophs. These cultures invariably harbored a small rod-shaped bacterium with a mean cell volume of 0.05 to 0.06 μm3 and an apparent DNA content of 1 to 1.5 fg per cell. This cell type had the dimensions of ultramicrobacteria. Isolates of these ultramicrobacterial cultures that were eventually obtained on relatively high-nutrient agar plates were, with respect to cell volume and apparent DNA content, identical to the cells in the initially obligately oligotrophic bacterial dilution culture. Determination of kinetic parameters from one of these small rod-shaped strains revealed a high specific affinity for the uptake of mixed amino acids (a°A, 1,860 liters/g of cells per h), but not for glucose or alanine as the sole source of carbon and energy (a°A, ± 200 liters/g of cells per h). The ultramicrobial strains obtained are potentially a very important part of picoplankton biomass in the areas investigated. 相似文献
139.
Influence of Metronidazole, CO, CO2, and Methanogens on the Fermentative Metabolism of the Anaerobic Fungus Neocallimastix sp. Strain L2 下载免费PDF全文
Femke D. Marvin-Sikkema Elizabeth Rees Marjan N. Kraak Jan C. Gottschal Rudolf A. Prins 《Applied microbiology》1993,59(8):2678-2683
The effects of metronidazole, CO, methanogens, and CO2 on the fermentation of glucose by the anaerobic fungus Neocallimastix sp. strain L2 were investigated. Both metronidazole and CO caused a shift in the fermentation products from predominantly H2, acetate, and formate to lactate as the major product and caused a lower glucose consumption rate and cell protein yield. An increased lactate dehydrogenase activity and a decreased hydrogenase activity were observed in cells grown under both culture conditions. In metronidazole-grown cells, the amount of hydrogenase protein was decreased compared with the amount in cells grown in the absence of metronidazole. When Neocallimastix sp. strain L2 was cocultured with the methanogenic bacterium Methanobrevibacter smithii, the fermentation pattern changed in the opposite direction: H2 and acetate production increased at the expense of the electron sink products lactate, succinate, and ethanol. A concomitant decrease in the enzyme activities leading to these electron sink products was observed, as well as an increase in the glucose consumption rate and cell protein yield, compared with those of pure cultures of the fungus. Low levels of CO2 in the gas phase resulted in increased H2 and lactate formation and decreased production of formate, acetate, succinate, and ethanol, a decreased glucose consumption rate and cell protein yield, and a decrease in most of the hydrogenosomal enzyme activities. None of the tested culture conditions resulted in changed quantities of hydrogenosomal proteins. The results indicate that manipulation of the pattern of fermentation in Neocallimastix sp. strain L2 results in changes in enzyme activities but not in the proliferation or disappearance of hydrogenosomes. 相似文献
140.
Misuses of the single cosinor analysis are numerous. We give three rules of thumb, providing guidelines as to whether results obtained from this analysis are dubious. This short note avoids mathematical development so as to be comprehensible to the nonspecialist. However, a few endnotes, give some technical comments. 相似文献