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81.
Rajesh Kumar G Mrudula Spurthi K Kishore Kumar G Mohanalatha Kurapati Saraswati M Mohini Aiyengar T Chiranjeevi P Srilatha Reddy G Nivas S Kaushik P Sanjib Sahu K Surekha Rani H 《PloS one》2015,10(3)
Background
Coronary Artery Disease (CAD) is clearly a multifactorial disease that develops from childhood and ultimately leads to death. Several reports revealed having a First Degree Relatives (FDRS) with premature CAD is a significant autonomous risk factor for CAD development. C - reactive protein (CRP) is a member of the pentraxin family and is the most widely studied proinflammatory biomarker. IL-18 is a pleiotrophic and proinflammatory cytokine which is produced mainly by macrophages and plays an important role in the inflammatory cascade.Methods and Results
Hs-CRP levels were estimated by ELISA and Genotyping of IL-18 gene variant located on promoter -137 (G/C) by Allele specific PCR in blood samples of 300 CAD patients and 300 controls and 100 FDRS. Promoter Binding sites and Protein interacting partners were identified by Alibaba 2.1 and Genemania online tools respectively. Hs-CRP levels were significantly high in CAD patients followed by FDRS when compared to controls. In IL-18 -137 (G/C) polymorphism homozygous GG is significantly associated with occurrence of CAD and Hs-CRP levels were significantly higher in GG genotype subjects when compared to GC and CC. IL-18 was found to be interacting with 100 protein interactants.Conclusion
Our results indicate that Hs-CRP levels and IL-18-137(G/C) polymorphism may help to identify risk of future events of CAD in asymptomatic healthy FDRS. 相似文献82.
Pawel Nowak Derek C. Cole Ann Aulabaugh Jonathan Bard Rajiv Chopra Rebecca Cowling Kristi Y. Fan Baihua Hu Steve Jacobsen Minakshi Jani Guixan Jin Mei-Chu Lo Michael S. Malamas Eric S. Manas Rani Narasimhan Peter Reinhart Albert J. Robichaud Joseph R. Stock Joan Subrath Kristine Svenson John W. Ellingboe 《Bioorganic & medicinal chemistry letters》2010,20(2):632-635
8,8-Diphenyl-2,3,4,8-tetrahydroimidazo[1,5-a]pyrimidin-6-amine (1) was identified through HTS, as a weak (micromolar) inhibitor of BACE1. X-Ray crystallographic studies indicate the 2-aminoimidazole ring forms key H-bonding interactions with Asp32 and Asp228 in the catalytic site of BACE1. Lead optimization using structure-based focused libraries led to the identification of low nanomolar BACE1 inhibitors such as 20b with substituents which extend from the S1 to the S3 pocket. 相似文献
83.
84.
T. Sreelatha A. Hymavathi J. Madhusudhana Murthy P.U. Rani J. Madhusudana Rao K. Suresh Babu 《Bioorganic & medicinal chemistry letters》2010,20(9):2974-2977
A bioassay-guided fractionation and chemical examination of chloroform extract of Plumbago capensis roots resulted in isolation and characterization of two new napthaquinone derivatives (4, 8) along with six known compounds (1–3, 5–7). Their structures were determined on the basis of extensive spectroscopic (IR, MS, 1D and 2D NMR) data analysis and by comparison with the literature data. All the compounds were tested for their mosquito larvicidal activity against fourth instar larvae of Aedes aegypti, and compared with that of rotenone. Among the tested compounds, isoshinanolone (3) and plumbagin (1) showed excellent toxicity with LC50 values of 1.26 and 5.43 μg/mL. New compound (8) displayed moderate toxicity against the tested mosquito species. 相似文献
85.
Deepa Selvi Rani Perundurai S. Dhandapany Pratibha Nallari Periyasamy Govindaraj Lalji Singh Kumarasamy Thangaraj 《Mitochondrion》2010,10(2):166-173
Mutations in PTPN11 gene was responsible for ~50% of the Noonan syndrome (NS), however, we did not find any mutation in PTPN11 in any of seven NS patients analysed. Whereas, the complete mtDNA sequencing revealed 146 mutations, of which five, including one heteroplasmic (A11144R; Thr → Ala) non-synonymous mutation, were novel and exclusively observed in NS patients. Interestingly all the seven probands and their maternal relatives were clustered under a major haplogroup R and its novel sub-haplogroups (R7b1b, R30a1, R30c, T2b7, U9a1) exclusive in NS, therefore we strongly suggest that these haplogroups may influence NS in South Indian populations. 相似文献
86.
Dev Chandran Kolli Bhaktavatsala Reddy Shahana Pallichera Vijayan Parthasarthy Sugumar Gudavalli Sudha Rani Ponsekaran Santha Kumar Lingala Rajendra Villuppanoor Alwar Srinivasan 《Indian journal of microbiology》2010,50(3):266-274
Peste des Petits Ruminants (PPR) is a highly contagious animal disease caused by the Peste des Petits Ruminants virus (PPRV) belonging to the genus morbillivirus and family Paramyxoviridae. The disease results in high morbidity and mortality
in goats, sheep and in some small wild ruminants. The presence of large number of small ruminants reared in endemic areas
makes PPR a notorious disease threatening the livelihood of poor farmers. Conventional vaccination using a live, attenuated
vaccine gives adequate protection but cannot be used in case of eradication of the disease due to difficulty in differentiation
of infected animals from the vaccinated ones. 相似文献
87.
88.
Nangia-Makker P Nakahara S Hogan V Raz A 《Journal of bioenergetics and biomembranes》2007,39(1):79-84
During the past decade, extensive progress has been made toward understanding the molecular basis for the regulation of apoptosis.
In mammalian cells undergoing apoptosis, two distinct mechanisms or pathways are operated and are triggered by cell death
stimuli from intra- or extra-cellular environments, namely the intrinsic or extrinsic pathways, resulting in mitochondrial
membrane depolarization. Several lines of evidence from our laboratories and others have indicated that galectin-3 plays an
important role in these pathways by binding to various ligands. Here the authors provide a brief discussion on the role of
endogenous or extra-cellular galectin-3 in the regulation of apoptosis and how it could be used as a therapeutic target using
natural plant products as its functional inhibitors. 相似文献
89.
Tubulin, a heterodimeric (alphabeta) protein, the main constituent of microtubules, binds efficiently with colchicine (consisting of a trimethoxybenzene ring, a seven-member ring and methoxy tropone moiety) and its analogues, viz., demecolcine and AC [2-methoxy-5-(2',3',4'-trimethoxyphenyl)tropone]. Tubulin contains eight tryptophan (Trp) residues at A21, A346, A388, A407, B21, B103, B346, and B407 in the two subunits. The role of these eight Trp residues in this interaction and also their perturbation due to binding have been explored via time-resolved fluorescence at room temperature and low-temperature (77 K) phosphorescence in a suitable cryosolvent. Both the time-resolved fluorescence data and 77 K phosphorescence spectra indicate that the emitting residues move toward a more hydrophobic and less polar environment after complex formation. The environment of emitting Trps in the complex also becomes slightly more heterogeneous. Our analysis using the experimental results, the calculation of the accessible surface area (ASA) of all the Trps in the wild type and tubulin-colchicine complex [Ravelli, R. B. G., et al. (2004) Nature 428, 198-202], the distance of the Trp residues from the different moieties of the colchicine molecule, the knowledge of the nature of the immediate residues (<5 A) present near each Trp residue, and the calculation of the intramolecular Trp-Trp energy transfer efficiencies indicate that Trp A346, Trp A407, Trp B21, and Trp B407 are the major contributors to the emission in the free protein, while Trp B21 and Trp B103 are mainly responsible for the emission of the complexes. A comparative account of the photophysical aspects of the drug molecules bound to protein in aqueous buffer and in buffer containing 40% ethylene glycol has been presented. The quantum yield and average lifetime of fluorescence in tubulin and its complexes with colchicine are used to predict the possible donors and the energy transfer (ET) efficiency in the ET process from Trps to colchicine in the complex. This study is a unique attempt to identify the Trp residues contributing to the emission in the free protein and in a complex of a multi-Trp protein with a drug molecule without performing the mutation of the protein. 相似文献
90.
Keratinases degrade feather in presence of a suitable reducing agent. Here we have demonstrated that conventional serine and
cysteine proteases (subtilsin, chymotrypsin and papain) which selectively cleave proteins at the hydrophobic P1 residues also
degrade feathers in presence of a suitable reducing agent in the form of live cells or chemical reductants. Further, trypsin
and pepsin were also shown to degrade feather after cleaving hydrophobic residues of feathers following 2 h pre-treatment
by any of the proteases. 相似文献