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471.
Vassilios Tsikaris Evangelos Detsikas Maria Sakarellos-Daitsiotis Constantinos Sakarellos Efstratia Vatzaki Socrates J. Tzartos Michel Marraud Manh Thong Cung 《Biopolymers》1993,33(7):1123-1134
The conformational properties of two [D -A70, A76] and [Aib70, A76] analogues of the α67–76 Torpedo acetylcholine receptor fragment, with low binding capacity for the anti main immunogenic region (MIR) antibodies, were studied in DMSO by two-dimensional nmr techniques and molecular dynamics simulations. The results were compared to the free and bound conformations of the [A76] analogue, which has twice more affinity for the anti-MIR monoclonal antibody 6 (mAb6), than the natural Torpedo sequence. It appeared that a single substitution of the A70, at a crucial position, by the D -A70 or Aib70, could modify completely the conformational behavior of the peptide and reduced its recognition by the anti-MIR antibody. The WNPADY rigid structure at the N-terminal part was essential for antibody recognition. The adjacent more flexible C-terminal sequence (GGIK) gives additional stability to the monoclonal antibody–peptide complex probably due to an adequate orientation of the peptide side chains in the complex, by setting them in close contact with the antibody. © 1993 John Wiley & Sons, Inc. 相似文献
472.
473.
Spyridon Ntougias Nektarios Kavroulakis Kalliope K. Papadopoulou Constantinos Ehaliotis Georgios I. Zervakis 《Journal of microbiology (Seoul, Korea)》2010,48(3):297-306
Microbial assessment of grape marc wastes, the residual solid by-product of the wine-industry, was performed by identifying
phylogenetically the fungal culturable diversity in order to evaluate environmental and disposal safety issues and to discuss
ecological considerations of applications on agricultural land. Fungal spores in grape marc were estimated to 4.7×106 per g dry weight. Fifty six fungal isolates were classified into eight operational taxonomic units (OTUs) following amplified
ribosomal DNA restriction analysis (ARDRA) and colony morphology. Based on 18S rRNA gene and 5.8S rRNA gene-ITS sequencing,
the isolates representing OTUs #1, #2, #3, and #4, which comprised 44.6%, 26.8%, 12.5%, and 5.3%, respectively, of the number
of the total isolates, were identified as Aspergillus fumigatus, Bionectria ochroleuca, Haematonectria haematococca, and Trichosporon mycotoxinivorans. The isolates of OTU#5 demonstrated high phylogenetic affinity with Penicillium spp., while members of OTUs #6 and #7 were closer linked with Geotrichum candidum var. citri-aurantii and Mycocladus corymbifer, respectively (95.4 and 97.9% similarities in respect to their 5.8S rRNA gene-ITS sequences). The OTU#8 with a single isolate
was related with Aspergillus strains. It appears that most of the fungal isolates are associated with the initial raw material. Despite the fact that
some of the species identified may potentially act as pathogens, measures such as the avoidance of maintaining large and unprocessed
quantities of grape marc wastes in premises without adequate aeration, together with its suitable biological treatment (e.g.,
composting) prior to any agriculture-related application, could eliminate any pertinent health risks. 相似文献
474.
475.
Athanassios Stavrakoudis Ioannis N. Demetropoulos Constantinos Sakarellos Maria Sakarellos-Daitsiotis Vassilios Tsikaris 《International journal of peptide research and therapeutics》1997,4(4-6):481-487
Summary The design, synthesis and catalytic properties of a cyclic branched peptide carrier that possesses the catalytic triad residues
of the serine proteases is reported. The synthesis of the peptide model was totally completed on solid support using three
different orthogonal amino protecting groups. Hydrolytic activity measurements against Suc-Ala-Ala-Ala-pNA substrate showed
that it is hydrolysed by the peptide model to a small extent. Despite this small hydrolytic activity, it is the first time,
to our knowledge, that hydrolysis of such a substrate is reported by an enzyme model compound. Contrary, this enzyme model
peptide showed considerable activity against the Boc-Ala-pNP substrate (k
cat
=0.414 min−1 andK
m
=0.228 mm). These results suggest that the designed carrier brings in appropriate contact the catalytic triad residues (Ser,
His, Asp) resulting in the obtained hydrolytic activity. 相似文献