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51.
Izquierdo-Useros N Lorizate M Contreras FX Rodriguez-Plata MT Glass B Erkizia I Prado JG Casas J Fabriàs G Kräusslich HG Martinez-Picado J 《PLoS biology》2012,10(4):e1001315
HIV-1 is internalized into mature dendritic cells (mDCs) via an as yet undefined mechanism with subsequent transfer of stored, infectious virus to CD4+ T lymphocytes. Thus, HIV-1 subverts a DC antigen capture mechanism to promote viral spread. Here, we show that gangliosides in the HIV-1 membrane are the key molecules for mDC uptake. HIV-1 virus-like particles and liposomes mimicking the HIV-1 lipid composition were shown to use a common internalization pathway and the same trafficking route within mDCs. Hence, these results demonstrate that gangliosides can act as viral attachment factors, in addition to their well known function as cellular receptors for certain viruses. Furthermore, the sialyllactose molecule present in specific gangliosides was identified as the determinant moiety for mDC HIV-1 uptake. Thus, sialyllactose represents a novel molecular recognition pattern for mDC capture, and may be crucial both for antigen presentation leading to immunity against pathogens and for succumbing to subversion by HIV-1. 相似文献
52.
Carlos Felipe Bosa Alba Marina Cotes Prado Takehiko Fukumoto Marie Bengtsson & Peter Witzgall 《Entomologia Experimentalis et Applicata》2005,114(2):137-142
Chemical analysis of pheromone gland extracts followed by behavioural studies in the wind tunnel and by field trapping tests show that the sex pheromone of the Guatemalan potato moth, Tecia (Scrobipalpopsis) solanivora Povolny (Lepidoptera: Gelechiidae), is a blend of (E)‐3‐dodecenyl acetate, (Z)‐3‐dodecenyl acetate, and dodecyl acetate. A 100 : 1 : 20 blend of these compounds, formulated at 1000 µg on rubber septa, captured more males than the main compound alone. This lure was species‐specific and did not capture the potato tubermoth, Phthorimaea operculella. A potato field was treated with a blend of these three compounds at a rate of 28 g ha?1. Male T. solanivora attraction to synthetic pheromone traps was almost completely suppressed for 2 months, demonstrating the potential use of pheromones for control of this economically important insect pest of potato in Central and Southern America. 相似文献
53.
Fernando Ribas Prado Claude Giessner-Prettre Bernard Pullman 《Journal of theoretical biology》1978,74(2):259-277
The variation of the chemical shift of the protons of 5′-UMP and 5′-AMP is calculated as a function of χCN, ψ and ? torsion angles. The shift of H8 of 5′-AMP and H6 of 5′-UMP is found to be very sensitive to the value of χCN. For the anti conformations the shift of these protons is more sensitive to the value of the rotation about CS′-05′ than about C4′-CS′. For the protons of the ribose the calculations show that for the C2′-endo pucker H3′ and H2′ undergo the largest chemical shift variations when ? and ψ vary. The calculated variations are considered in relation with the role of the conformation of the nucleotides in the chemical shift variation between mono and polynucleotides and between the different helical structures of polynucleotides. 相似文献
54.
Differences in sperm morphology in foam‐nesting leptodactyline frogs (Anura,Leptodactylidae) 下载免费PDF全文
Sperm morphology is diverse among vertebrates and is influenced by the reproductive strategies adopted by species. In anurans, sperm morphology is associated with reproductive modes and mating systems. Here, we describe the sperm morphology of 11 frog species in the genus Leptodactylus and that of Lithodytes lineatus and discuss the relationship between sperm morphology and species' mating systems. We observed two distinct sperm morphotypes among the leptodactyline species, which differed mostly in head morphology. Type I sperm had triangular head, discrete acrosome vesicle with posterior margin not clearly visible; type II sperm had elongated head, clear acrosomal vesicle with posterior margin clearly visible. These sperm types do not seem to be associated with phylogeny; instead, type II sperm was observed in all polyandrous species analysed and in species with evidences of polyandry. Moreover, sperm of all species presented tail with undulating membrane connected to the axial fibre. We suggest that differences in sperm morphology might be associated with sperm competition to what polyandrous species are subjected. However, natural history observations on polyandrous mating in some species presenting type II sperm and phylogenetic comparative studies are need to elucidate the role of mating systems in the evolution of sperm morphology in leptodactylines. 相似文献
55.
A global plastid phylogeny of the brake fern genus Pteris (Pteridaceae) and related genera in the Pteridoideae 下载免费PDF全文
56.
P Venault L Prado de Carvalho C L Brown R H Dodd J Rossier G Chapouthier 《Life sciences》1986,39(12):1093-1100
Certain pharmacological properties of methyl beta-carboline-3-carboxylate (beta-CCM), a benzodiazepine receptor ligand, have been investigated in chicks. Although beta-CCM has been established previously as an "inverse agonist" of benzodiazepine receptors in rodents, having effects opposite to those of benzodiazepines in a variety of tests, in chicks this compound had a different pharmacological profile. Firstly, in contrast to the overt convulsant action of beta-CCM in other species, beta-CCM (0.05-40 mg/kg) did not produce convulsions by itself in chicks, but it was only proconvulsant. Secondly and most surprisingly, beta-CCM, like diazepam, produced in chicks a sedation which could be blocked by the benzodiazepine receptor antagonist Ro 15-1788. Thus it appears that beta-CCM can function both as an agonist and as an inverse agonist in this animal. 相似文献
57.
Background
Simple models inspired by processes shaping consumer-resource interactions have helped to establish the primary processes underlying the organization of food webs, networks of trophic interactions among species. Because other ecological interactions such as mutualisms between plants and their pollinators and seed dispersers are inherently based in consumer-resource relationships we hypothesize that processes shaping food webs should organize mutualistic relationships as well.Methodology/Principal Findings
We used a likelihood-based model selection approach to compare the performance of food web models and that of a model designed for mutualisms, in reproducing the structure of networks depicting mutualistic relationships. Our results show that these food web models are able to reproduce the structure of most of the mutualistic networks and even the simplest among the food web models, the cascade model, often reproduce overall structural properties of real mutualistic networks.Conclusions/Significance
Based on our results we hypothesize that processes leading to feeding hierarchy, which is a characteristic shared by all food web models, might be a fundamental aspect in the assembly of mutualisms. These findings suggest that similar underlying ecological processes might be important in organizing different types of interactions. 相似文献58.
Angel Casacó Irene Beausoleil Bárbara Gonzalez Patricia Luaces Avelina León María E. Arteaga Pavel Prado Valia Rodríguez Aylén Pérez Grisel Guevara Ana M. Bada Nuris Ledón Dasha Fuentes Consuelo González Osvaldo Hernandez Romy Orphee Diuris Blanco Miriam García‐Osuma Alcides Ballester‐Labrada 《Journal of medical primatology》2010,39(3):177-186
Background The purpose of this study is to better characterize the hematological, biochemical, respiratory, cardiovascular and electroneurophysiological parameters in young adult Cercopithecus aethiops sabaeus of both sexes. The rhesus and cynomolgus monkeys are widely used as experimental primate models. However, only few articles have been published testing toxicological effects of pharmaceuticals on African green monkey. Methods The present study was carried out with the recompilation of all parameters recorded before the first drug administration in five sub‐chronic or chronic toxicological studies performed on 66 Cercopithecus aethiops sabaeus, born in Cuba. Results This study provides hematological, biochemical, respiratory, cardiovascular and electroneurophysiological data for both choosing animals to be included into experiments and monitoring these parameters during the study. Conclusions We conclude that this study provides valuable integrated data for determining the health status, including electroneurophysiological parameters, data not previously reported for this species, of the African green monkey. 相似文献
59.
Simone S. Prado Kim Y. Hung Matthew P. Daugherty Rodrigo P. P. Almeida 《Applied and environmental microbiology》2010,76(4):1261-1266
Impacts of climate change on organisms are already apparent, with effects ranging from the individual to ecosystem scales. For organisms engaged in mutualisms, climate may affect population performance directly or indirectly through mediated effects on their mutualists. We tested this hypothesis for two stink bugs, Acrosternum hilare and Murgantia histrionica, and their gut-associated symbionts. We reared these species at two constant temperatures, 25 and 30°C, and monitored population demographic parameters and the presence of gut-associated symbionts with diagnostic PCR primer sets. Both stink bugs lost their respective gut symbionts within two generations at 30°C. In addition, the insect survivorship and reproductive rates of both A. hilare and M. histrionica at 30°C were lower than at 25°C. Other demographic parameters also indicated a decrease in overall insect fitness at the high temperature. Collectively our data showed that the decrease in host fitness was coupled with, and potentially mediated by, symbiont loss at 30°C. This work illustrates the need to better understand the biology of animal-symbiont associations and the consequences of local climate for the dynamics of these interactions.The effect of climate on organisms, communities, and the environment at large has become a pressing issue for biologists and environmental scientists. Recent studies indicate that previous forecasts were conservative in their predictions for the magnitude of global warming (29). Up-to-date models suggest that the global mean surface temperature will increase by 1.8 to 4°C by the year 2100 (11). The ecological impact of such warming is already apparent (35) in the effects seen on species fitness (24), range shifts (22), species interactions (10), and community structure (32).It is important to note that many macroorganisms live in symbiosis with microbes and that host fitness may be affected indirectly by higher temperatures due to the disruption of mutualistic relationships. Some corals, for example, have symbiotic relationships with photosynthesizing dinoflagellates (zoothanxellae) that provide them with nutrients (31). Higher water temperatures in reef ecosystems, among other factors, induce the expulsion of microbial symbionts by the host, resulting in coral bleaching (15). Therefore, it is plausible that observed effects of climate on species distribution or performance might stem from disruption of symbiotic interactions as much as from direct effects on host biology.Despite the current interest in insect-microbe symbioses, the vast majority of such systems have been poorly studied. A group of insects that has recently received some attention are the true bugs (Hemiptera, Pentatomomorpha). Studies in the early 1900s suggested that mutualistic bacteria colonized a portion of the gut of insects in different pentatomomorphan families (9). More specifically, monocultures of bacteria were present in high densities in the crypt- (or cecum-) bearing organ preceding the hindgut of hosts, with different bacterial taxa associated with different true bug families. Furthermore, studies have shown that these symbionts are mutualistic (1, 8, 13, 14, 26). Among these bug families, the stink bugs (Pentatomidae) have been shown to depend on their gut symbionts (1). Pentatomid symbionts are polyphyletic and closely related to plant-associated bacteria in the genera Erwinia and Pantoea (25). Although the mechanism of symbiont vertical transmission is poorly understood, females seem to smear the surface of eggs with bacteria while ovipositing (3). Aposymbiotic first instars hatch but remain on the surface of eggs and acquire the symbiont by probing on the egg surface, as evidenced by the fact that surface sterilization of egg masses generates aposymbiotic individuals (1, 26, 28).Climate change has already affected stink bug performance and geographic range (19, 20). In Japan, populations of two pentatomid species, Nezara viridula and N. antennata, have shifted northwards and to higher elevations, respectively, over the last 50 years (33). However, we found previously that for one of these species high temperature eliminated gut symbionts, without any clear decrease in host fitness (27). Thus, it remains unclear whether temperature change played a role, either directly or indirectly, in these geographic shifts. To better understand the extent to which temperature mediates stink bug ecology and prevalence of their gut bacteria, we conducted laboratory studies with two pentatomid species, Acrosternum hilare and Murgantia histrionica. We show that high temperature affects the symbiotic relationship, with concomitant reduction in insect fitness. 相似文献
60.
Raquel Gutiérrez-González Gregorio R Boto Cristina Fernández-Pérez Náyade del Prado 《BMC neurology》2010,10(1):93