The Species Problem: Nineteenth-Century Concepts of Racial Inferiority in the Origin of Man Controversy

The species problem and its implications in the origin of man controversy had grown in importance in prewar America owing largely to the question of slavery. Implicit in the problem was the position of the so-called inferior races in society. The monogenists, despite their emphasis on environmentalism, were no more favorable to the Negro, except in their remote theoretical stance. The Civil War—not Darwin—brought the controversy to an end in America, but it continued to rage in Europe. The apparent synthesis of the schools during the 1870s did not disturb the stereotyped ideas of racial inferiority. The "inferior races" remained the basis of evolutionary discussion, leaving them as remote outcasts of the evolutionary struggle.     

Ecology and Evolution of Social Organization in Arctic Sandpipers

A comparative analysis of sandpiper social systems on arcticand subarctic breeding grounds (24 species in the family Scolopacidae,subfamily Calidridinae) shows four major patterns. In a majorityof the species (15), populations are dispersed through a stronglydeveloped territorial system, with strong monogamous pair bondsand only minor yearly fluctuations in numbers. The second patternis seen in three species in which the female of a pair may laytwo sets of eggs in quick succession, one for each member ofthe pair to incubate. This opens opportunities for facultativepolygyny or polyandry (‘serial polygamy’) and forthe evolutionary weakening of the strong pair bond seen in thefirst pattern. The third and fourth patterns are those of polygyny(three species) and promiscuity (three species). These six speciesshow clumped dispersions; their year-to-year fluctuations tendto be strong; the males defend compressible, often small, territories;and high densities can occur locally. It is suggested that thepattern of overdispersion and monogamy represents a conservativemode of adapting to high-latitude environments, while the patternof clumped dispersion with polygyny or promiscuity representsan opportunistic mode in that the birds are concentrated intobreeding areas where and when weather, food, and/or some otherenvironmental factors are particularly favorable. Apparentlyfalling evolutionarily between these two basic patterns areseveral species conservative in their life-styles, but polygamousat least occasionally and showing some features of opportunism.There is thus a striking diversity of social systems in calidridinesandpipers, that is, in the styles of habitat exploitation theyhave evolved in the arctic and subarctic habitats to which theirbreeding is confined. A graphic model suggesting paths of evolutionarydevelopment and of interplay among factors considered criticalin the evolution of these systems is proposed.     

Growth Rate and Water Relations of Citrus Leaf Flushes

Growth rates of seasonal leaf flushes of ‘Valencia’orange [Citrus sinensis (L.) Osbeck] were measured and waterrelations characteristics of young (new) and over-wintered (old)citrus leaves were compared. New flush leaves had lower specificleaf weights and lower midday leaf water potentials than comparablyexposed old leaves. Spring and summer flush new leaves had higherosmotic potentials than old leaves. These differences becamenon-significant as the new leaves matured. During summer conditions,water-stressed new leaves reached zero turgor and stomatal conductancealso began to decrease in them at higher leaf water potentialsthan in old leaves. Old leaves were capable of maintaining openstomata at lower leaf water potentials. Opened flowers and newflush leaves lost more water, on a dry weight basis, than flowerbuds, fruit or mature leaves. The results illustrate differencesin leaf water potential and stomatal conductance which can beattributed to the maintenance of leaf turgor by decreases inleaf osmotic potentials as leaves mature. These changes in citrusleaf water relations are especially important since water stressresulting from high water loss rates of new tissues could reduceflowering and fruit set. Citrus sinensis (L.) Osbeck, orange, Citrus paradisi Macf., grapefruit, growth rate, leaf water relations, osmotic potential, water potential, stomatal conductance     

Micronuclear DNA from Paramecium tetraurelia: Serotype 51 A Gene Has Internally Eliminated Sequences

A method for the isolation of micronuclear DNA from Paramecium tetraurelia has been developed. After cell lysis, a low speed centrifugation at 1,000 g is used to remove all of the unbroken cells and macronuclei and approximately two thirds of the macronuclear fragments. Next a higher speed centrifugation of 9,000 g sediments the micronuclei and frees them from small particulates and soluble constituents. Advantage is then taken of the fact that micronuclei have a lower density than do macronuclear fragments in 45%–60% Percoll. Micronuclei float to the top during centrifugation at 24,000 g , while macronuclear fragments sediment. After several cycles of centrifugation in Percoll, the micronuclei, although heavily contaminated with cytoplasmic components, are essentially free of macronuclei and macronuclear fragments. Micronuclear DNA can then be extracted from the suspension. The whole procedure is very rapid and in about an hour micronuclear and macronuclear DNA can be separated. About 2 μ g of micronuclear DNA can be obtained from 6 times 10⁷ paramecia. We find that there are internal sequences in the micronuclear A gene DNA in wild type cells which are eliminated when the micronuclei develop into macronuclei. They yield unique restriction fragments for micronuclei and macronuclei. Therefore the purity of the preparations is easily monitored by probing Southern blots of restriction enzyme-digested DNA with the cloned A gene. No differences have been found between the micronuclear A gene in wild type and the d48 mutant.     

Regulation of Prolactin and Growth Hormone Production by Clonal Strains of Functional Pituitary Cells in Culture

This report describes the use of clonal strains of rat pituitarytumor cells to study the regulation of prolactin and growthhormone production. Emphasis is placed on the effects on prolactinproduction of the hypothalamic tripeptide pGlu-His-ProNH₂, alsocalled thyrotropin releasing hormone (TRH). TRH binds to specificcellular receptors and stimulates initially the release of previouslysynthesized prolactin; several hours later it stimulates thesynthesis of prolactin. Several kinds of experimental resultsare consistent with the hypothesis that cyclic AMP mediatesthe TRH-stimuIated release of prolactin. However, all of theeffects of TRH on these cells in culture are not mimicked bycyclic AMP analogs, for TRH decreases growth hormone productionwhile it increases the synthesis of prolactin, and the analogdibutyryl cyclic AMP increases the production of both hormones.     

Geometric constraints and spatial pattern of species richness: critique of range-based null models

Abstract. Does the shape of a biogeographical region influence its spatial patterns of species richness? A complete answer must include careful distinction between the distribution of a species, which is a complex geometric object, and the range of a species, which is relatively simple, especially when reduced to one dimension. We consider range‐based models of species richness, in particular range overlap counts in one dimension, for which we give a unified mathematical treatment via the joint probability P(m,l) of midpoints and lengths of ranges. We discuss a number of difficulties, in practice and in principle, using range‐based models, and show that the so‐called mid‐domain effect, a proposed null model for the effect of geometric constraint, is qualitatively a property of all biologically realistic models based on range overlap counts. As such, range‐based models provide little insight into understanding or explaining biogeographical patterns in species richness. We characterize the quantitative null model for range overlap counts in one dimension, for which we give a simple and direct field test based on P(m,l). We apply this test to a large clade in a complete bioregion (the Proteaceae of the Cape Floristic Region): geometric constraint does not explain the spatial pattern in this case. We show that any geometric constraint on species richness, including range overlap counts, must act via edge effects. Thus, to understand biogeographical patterns, an understanding of the effects and consequences of edges is fundamental.