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221.
222.
Prabhakaran Vasantha-Srinivasan Sridhar Shanmuga-Priya Yeon Soo Han Narayanaswamy Radhakrishnan Kesavan Amala Sengodan Karthi Sengottayan Senthil-Nathan 《Entomological Research》2023,53(11):485-508
Extracts of botanical origin naturally contain a complex mixture of chemicals considered effective in managing lepidopteran pests. Chemical screening of the ethanolic leaf extracts of two fig tree species, Ficus lyrata and Ficus auriculata, delivered 12 and 15 phyto-compounds with relatively high peak area percentages in phytol and flavone, respectively. Larvicidal activity against Spodoptera litura yielded higher mortality rates at maximum-concentration treatment (600 ppm) with Ficus lyrata (91.3%) and Ficus auriculata (98.5%) extracts during the second instar. Sub-lethal dosages (300 ppm) of both Ficus lyrata and Ficus auriculata extracts impeded the development and reproduction of lepidopteran pests. The enzyme-inhibition activity of both fig extracts elicited a significant reduction in the major digestive enzymes alkaline phosphatase, acid phosphatase, and lactate dehydrogenase dose-responsively. Mid-gut histological screening of Ficus lyrata and Ficus auriculata extracts displayed gut lumen disruptions, shape alterations of columnar cells, and brush-border membrane damage. Further, the non-target toxicity of fig extracts against the red worm Eisenia foetida was minimal compared with that of the chemical temephos. Overall, the Ficus extracts proved to be eco-friendly strategies for managing the polyphagous pest Spodoptera litura and are potentially more sustainable and less harmful to non-target earthworms. Nonetheless, in silico predictions suggest that the active compounds in fig extracts are predominantly toxic against honeybees (16 compounds) and violate TICE rules (10 compounds). Therefore, the biological actions of fig extracts' individual novel chemistries need to be examined on target and non-target species to develop better pest management strategies. 相似文献
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224.
K. Vedhagiri K. Natarajaseenivasan P. Chellapandi S.G. Prabhakaran Joseph Selvin S. Sharma P. Vijayachari 《基因组蛋白质组与生物信息学报(英文版)》2009,7(3)
Leptospirosis is recognized as the most widespread zoonosis with a global distribution. In this study, the antigenic variation in Leptospira interrogans and Leptospira borgpetersenii isolated from human urine and field rat kidney was preliminarily confirmed by microscopic agglutination test using monoclonal antibodies, and was further subjected to amplification and identification of outer membrane lipoproteins with structural gene variation. Sequence similarity analysis revealed that these protein sequences... 相似文献
225.
The derivation of the restricted intra-sire regression heritability estimator is provided. Procedures for obtaining a stable estimate of residual error variance σ2 are outlined. A small illustration based on live data is given. 相似文献
226.
E.B. Harris R.M. Sanchez C.K. Job K. Prabhakaran R.C. Hastings 《FEMS microbiology letters》1990,70(1):95-99
Water and soil samples were collected from natural habitats of the nine-banded armadillo and tested for the presence of acid-fast organisms by injection into the foot pads of experimental mice. Sixteen months post inoculation an acid-fast organism was isolated from the foot pad and spleen of one of the mice. The isolate exhibited diphenoloxidase activity as determined by its ability to convert D-3,4-dihydroxyphenylalanine to the corresponding quinone. The same organisms grown in vitro lacked detectable diphenoloxidase activity. However, diphenoloxidase activity was observed in acid-fast organisms harvested from spleen tissue of mice experimentally inoculated with a pure culture of the isolate. The environmental isolate was tentatively classed with the Mycobacterium avium-intracellulare complex. 相似文献