Behavioral Responses of Nursing Home Residents to Visits From a Person with a Dog,a Robot Seal or aToy Cat

Previous studies suggest that contact with dogs can positively affect the wellbeing of elderly people in nursing homes, but there is a lack of research investigating the causal pathways of these effects. One such path- way may relate to the behavioral responses of the elderly when interacting with a dog. The present study compared the immediate behavioral responses of nursing home residents to bi-weekly visits from a person accompanied by either a dog, a robot seal (PARO®), or a soft toy cat, using a randomized controlled design. A total of 100 nursing home residents com- pleted the study. Each participant received a total of 12 visits, during which their behaviors, including interactions between the visitor and the accom- panying animal (real or artificial), were recorded. Also, data on cognitive im- pairment, presence of depressive symptoms, age, time lived in the nursing home, dementia diagnoses, and gender were collected. We found that the immediate responses to, and interaction with, the visiting animal depended on the type of animal that was brought along. The dog and the interactive robot seal triggered the most interaction, in the form of physical contact (F(2,103) = 7.50, p < 0.001), eye contact (F(4,151) = 6.26, p < 0.001), and verbal communication (F(4,195) = 2.87, p < 0.05). As well, the cognitive impairment level of the residents affected with whom they interacted. The higher the cognitive impairment level, the more interaction was directed toward the animal and less toward humans, regardless of visit type (F(2,101) = 4.10, p < 0.05). The dogs and the robot seal stimulated the residents to more in- teraction, compared with the toy cat, but the robot seal failed to maintain the attention at the same level over time. The cognitive functioning of the residents correlated with the level of interaction, and this needs to be studied further.     

Molecular analysis of two Ypt/Rab-related sequences isolated from soybean (Glycine max) DNA libraries

From nodule and seedling cDNA libraries we isolated cDNA copies of two mRNAs, derived from the genes gmrl and gmr2, encoding members of the Ypt/Rab family of small GTP-binding proteins. Two deduced protein products, GMR1 and GMR2, were found to be nearly identical differing by only four amino acids in the analysed parts. The two putative proteins are 79% identical to the previously described ARA small GTPase from Arabidopsis thaliana. The GMR proteins may thus be the counterpart of the ARA protein and may perform a related biological function in Glycine max. The gmr2 genomic sequence was isolated and structurally analysed. Expression analyses by northern and cDNA-based PCR showed that the gmr1 and gmr2 genes are constitutively expressed in different plant organs, although at a slightly higher level in callus culture. The classification of the gmr sequences as relatives of the Ypt/Rab family suggests that the deduced GMR proteins are involved in control of processes related to vesicle trafficking in plant cells.     

Effects of membrane stabilizers on pancreatic amylase release.

Compounds with membrane stabilizing activity were studied as to their ability to affect pancreatic amylase release and the steps in the stimulus-secretion coupling process. Chlorpromazine, propranolol, and thymol were all found to inhibit bethanechol-stimulated amylase release and at slightly higher concentrations to induce release regardless of the presence of the secretagogue. This biphasic effect was similar to that found previously for the local anesthetic tetracaine. Release by high concentrations of propranolol and tetracaine was accompained by ultrastructural evidence of cell damage. Membrane stabilizers at concentrations which inhibited amylase release were shown to block bethanechol-induced depolarization and stimulation of 45Ca++ efflux although the drugs alone partially depolarized pancreatic cells. Release of amylase induced by Ca++ introduced by the ionophore A23187 was also abolished. The findings indicate that membrane stabilizers independently inhibit the steps leading to a rise in intracellular Ca++ and the subsequent Ca++-activated amylase release.     

Analysis of fluorescent pseudomonads based on 23S ribosomal DNA sequences.

The regions from positions 1391 to 1545 and 1620 to 1865 (Escherichia coli numbers) of the 23S ribosomal DNA sequences have been analyzed for a number of Pseudomonas fluorescens and P. putida isolates. Variability was observed only in three smaller regions from positions 1484 to 1508, 1531 to 1542, and 1714 to 1748, corresponding to helices 58, 59, and 63, respectively, where up to 53% dissimilarity was found. Sequence analysis did not allow clear distinctions among P. fluorescens biovars, P. chlororaphis, and P. putida biovar B.     

Immunocytochemical localization of renin in mouse kidney

Summary The distribution of renin in mouse kidney was examined in immunohistochemical studies by using an antiserum against pure mouse submaxillary renin and the peroxidase-antiperoxidase (PAP) technique. At antibody dilutions from 1:10⁴ to 1:10⁶, renin was found in high concentrations in the epitheloid cells of the vasa afferentia and, in lower concentrations, in the wall of some of the vasa efferentia. Renin was also detected in most of the interlobular arteries. Mesangial cells and Goormaghtigh cells were always free of specific staining. At high antiserum concentrations (i.e., dilutions from 1:10² to 1:10⁴) specific reaction product was also observed in the apical part of proximal tubule cells. This staining may represent filtered and pinocytozed renin.     

The high resolution R-banded karyotype of the domestic pig, Sus scrofa domestica L

A representative haploid R-banded karyotype of the domestic pig, and a diagrammatic representation of the banding patterns at the 550 band level are presented.     

Thyroid hormone metabolism in hypermetabolic patients with haematological disorders

Turnover tracer studies of T4 and T3 using the single injection, noncompartmental approach were performed in 6 hypermetabolic patients with haematological disorders (HHD) (basal metabolic rate (BMR): median 141%, range 122-166%), in 10 controls with stable, nonthyroidal illness (NTIC), and in 14 healthy controls (HC). The main finding was an increase of approximately 30% of the production rate (PR) of both T4 and T3 in patients with HHD. Median PR of T4 was 134 nmol/day x 70 kg in HHD, compared to 78 nmol/day x 70 kg in NTIC (P less than 0.05) and 98 nmol/day X 70 kg in HC (p less than 0.1), whereas median PR of T3 was 40.3 nmol/day x 70 kg in HHD, compared to 25.6 nmol/day x 70 kg in NTIC (P less than 0.01) and 31.1 nmol/day x 70 kg in HC (P less than 0.1). An increase of similar magnitude was found for the apparent distribution volume and the pool size of both T4 and T3. In contrast, the mean transit times of the hormones were similar in the 3 groups. Patients with HHD had normal levels of basal serum TSH as well as of the TSH response to TRH. Only PR of T3 correlated to the BMR (R = 1.00, P less than 0.02). The data are compatible with an increased consumption of thyroid hormones by malignant haematologic cells, and the increase of BMR seems to be dependent on the production of T3.