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101.
The activities of ornithine decarboxylase (ODC) and tyrosine aminotransferase (TAT) were determined under conditions of feeding or fasting in the hepatomas and livers of rats bearing Morris hepatoma 5123-C. Prior to killing, the animals were entrained to a schedule of 12 hours of light followed by 12 hours of darkness with food (60% protein) available only during the first two hours of the dark period. With food available, ODC and TAT activities displayed diurnal oscillations in hepatomas and host livers, and in the livers of control (non-tumor bearing) animals, characterized by rapid increases in enzyme activity coincident with the onset of feeding followed by a decline to pre-feeding levels. When food was withheld the increase in ODC activity in host and control livers, and TAT activity in hepatoma, host and control livers was not evident. However, withholding food did not abolish the diurnal oscillation of ODC activity in hepatoma 5123-C. 相似文献
102.
Beatrice de Nechaud Joyce E. Becker Van R. Potter 《Biochemical and biophysical research communications》1976,68(1):8-15
Glucocorticoids at concentrations equal to or higher than 10?7M lead to an increase of alpha-fetoprotein production by an established cell line from Morris hepatoma 8994. These cells also secreted alphaM-fetoprotein into the culture medium but only after addition of at least 4×10?7M hydrocortisone or 5×10?8M dexamethasone. The effects on both fetoproteins were observed in spite of a decrease of cell multiplication and an increase of cell detachment. 相似文献
103.
The efficacy of oxamyl in controlling Heterodera schachtii on cabbage was determined by applying various contbinations of soil drenches at 6.7 kg (a.i.)/ha and foliar sprays at 0.04 kg (a.i.)/100 liters of water to cabbage seedlings. Pretransplant drenches provided some control of H. schachtii over a 13-week period. A single foliar spray of oxamyl 1 week before transplanting apparently prevented penetration of H. schachtii larvae; post-transplant sprays were relatively ineffective. A pretransplant or transplant drench combined with a foliar application 2 weeks after transplanting provided the most effective control. The effectiveness of drenches plus post-transplant sprays is probably due to the spray augmenting the action of the drench in inhibiting the development of larvae after penelration. 相似文献
104.
Synthesis, surface deposition, and secretion of immunoglobulins by Abelson virus-transformed lymphosarcoma cell lines. 总被引:25,自引:0,他引:25
Three Abelson virus-transformed lymphoma cell lines were established in tissue culture and the immunoglobulin biosynthesis by these cell lines was studied. Two of the cell lines (ABLS-1 and ABLS-5) were found to synthesize monomeric IgM molecules which were deposited in the cell membrane, probably to serve as an antigen receptor. The third cell line (ABLS-8) was found to synthesize membrane-associated IgM as well as cellular IgG molecules. In addition, these cell lines were found to synthesize a protein of 35,000 molecular weight which is also membrane-associated and which has the capability to bind the immunoglobulin (MAID). It is speculated that this protein might play a role in adapting the receptor immunoglobulin molecule to the hydrophobic environment of the cell membrane. The kinetics of amino acid incorporation into immunoglobulins by these cell lines show that they produce immunoglobulins at a rate which is two orders of magnitude smaller than plasmacytoma cells (MOPC 104E). These results suggest that Abelson virus transforms thymus-independent lymphocytes in various stages of maturation and these lymphocytes might be of B cell origin. The T lymphoma (P1798) used as a control cell line was found occasionally to produce minute amounts of immunoglobulin. 相似文献
105.
Brain Cell Biology - In lizards (Sceloporus undulatus), long term (13 or 19 weeks) acclimation to an environment of 6 °C produces a striking increase in the argyrophilic neurofibrillar network... 相似文献
106.
107.
Electroporation in biology: methods, applications, and instrumentation 总被引:14,自引:0,他引:14
H Potter 《Analytical biochemistry》1988,174(2):361-373
108.
109.
The ichthyoplankton of a seasonally closed estuary in temperate Australia. Does an extended period of opening influence species composition? 总被引:1,自引:0,他引:1
Fish eggs and larvae were collected monthly between September 1987 and April 1989 from sites throughout the main basin and within the saline regions of the two main tributary rivers of Wilson Inlet, a seasonally closed estuary in south-western Australia. Of the eggs, 43.7% belonged to Engraulis australis (Shaw) and the rest to unidentified teleosts. The larval fish assemblage comprised 17 families represented by 25 species. The Gobiidae contained the highest number of species (four) and contributed approximately 57% of all larvae caught. Pseudogobius olorum (Sauvage) and E. australis were the most abundant species, contributing 43.9 and 27.9% to the total larval catch, respectively. The larvae of species which breed within Wilson Inlet dominated the assemblage, both in terms of number of species (64%) and contribution to total catch (99.9%). The numbers of the eight marine species and one freshwater species represented in the ichthyoplankton were very low. Classification and multi-dimensional scaling ordination showed that the composition of the larval fish fauna at the various sites during a period when the estuary remained open to the sea (December 1988-April 1989) was similar to that of the corresponding sites during the same period in the previous year when the estuary had become closed (December 1987-April 1988). This can be attributed to the spatial distribution, time of occurrence and abundance of estuarine-spawned larvae being similar in the two periods and to the rarity of marine-spawned larvae, even in the spring and summer of 1988/1989 when the estuary was open for the whole time when most marine teleosts spawn in south-western Australia. The low occurrence of marine-spawned larvae in Wilson Inlet reflects the fact that tidal water movement within the basin of the system is so small that it is unable to facilitate the transport and dispersion of larvae. The ichthyoplankton of Wilson Inlet resembles that of other poorly-flushed estuaries in that it is low in species richness and dominated by estuarine-spawned larvae. 相似文献
110.
V. R. Potter 《The Yale journal of biology and medicine》1980,53(5):367-384
Three major theories of cancer--somatic mutation, virus causation, and faulty differentiation--are proposed to involve alterations in DNA structure. Each results finally in terms of failures in the normal intercellular communication that involves feedback between differentiated cells acting on less differentiated cells still capable of proliferation. The historical background of the latter idea is traced to Osgood, Weiss and Kavanau, and to Iversen. The historical background of concepts of initiation and promotion are traced to Berenblum and Mottram and the Boutwell concept of promotion as gene activation is cited. It is proposed that gene activation by promoters is a valid concept and that it results from the blocking of the normal intercellular communication postulated by Osgood and others. The problem of explaining the low probability of cancer following initiators or promoters acting alone is cited as a problem in basic science. A hypothesis to solve the problem is proposed: Cancer results from two of more relevant mutations: promoters enhance proliferation of cells with one relevant mutation, thereby increasing the probability of obtaining a cell with two relevant mutations. A new scheme of five stages of hepatocarcinogenesis is proposed in terms of the hypothesis and available data. 相似文献