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Rachel IM van Haaften Blanche Schroen Ben JA Janssen Arie van Erk Jacques JM Debets Hubert JM Smeets Jos FM Smits Arthur van den Wijngaard Yigal M Pinto Chris TA Evelo 《BMC bioinformatics》2006,7(1):200-15
Background
Gene expression microarray technology permits the analysis of global gene expression profiles. The amount of sample needed limits the use of small excision biopsies and/or needle biopsies from human or animal tissues. Linear amplification techniques have been developed to increase the amount of sample derived cDNA. These amplified samples can be hybridised on microarrays. However, little information is available whether microarrays based on amplified and unamplified material yield comparable results. 相似文献90.
In this study we have investigated whether proteoglycans (aggrecan) are modified by nonenzymatic glycation as in collagen.
Purified human aggrecan from osteoarthritic and normal human knee articular cartilage was assayed for pentosidine, a cross-link
formed by nonenzymatic glycation, using reverse-phase HPLC. In addition, an in vitro study was done by incubation of purified
bovine nasal cartilage aggrecan with ribose. Pentosidine was found in all the purified human aggrecan samples. 2-3% of the
total articular cartilage pentosidine was found in aggrecan. Purified link protein also contained penosidine. The in vitro
study led to pentosidine formation, but did not appear to increase the molecular size of the aggrecan suggesting that pentosidine
was creating intramolecular cross-links. Similar amounts of glycation were found in osteoarthritic and normal cartilage. Like
collagen, aggrecan and link proteins are crosslinked by nonenzymatic glycation in normal and osteoarthritic cartilage. Crosslinking
could be reproduced, in vitro, by incubating aggrecan with ribose.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献