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101.
Drozdov-Tikhomirov LN Linde DM Poroĭkov VV Aleksandrov AA Skurida GI Kovalev PV Potapov VIu 《Molekuliarnaia biologiia》2003,37(1):164-173
A software package was designed and used in a detailed study of the contact regions (interfaces) of a large number of protein-protein complexes using the PDB data. It appeared that for about 75% of the complexes the amino acid composition of the subunit surface in the contact region is not essential. Thus one may suggest that, along with the amino acid residues at the interface, the residues in the interior of the globules substantially contribute to protein-protein recognition. Such interactions between quite remote residues are most probably of electrical nature, and are involved in recognition by contributing to the overall electric field created by the protein molecule; the configuration of this field is perhaps the definitive factor of recognition. The overall field of the protein molecule is additively built of the fields created by each constituent residue, and it can be calculated as a sum of the fields created by the protein multipole (aggregate of 'partial' electric charges assigned to every atom of the protein molecule). Preliminary calculations of the remote electrostatic interaction have been performed for ribonuclease subunits in vacuum. The results are indicative of a real possibility that the electric field created by the protein multipole can strongly influence the mutual orientation of molecules before Brownian collisions. 相似文献
102.
Conversion of rainforest to oil palm and rubber plantations alters energy channels in soil food webs
Winda Ika Susanti Melanie M. Pollierer Rahayu Widyastuti Stefan Scheu Anton Potapov 《Ecology and evolution》2019,9(16):9027-9039
In the last decades, lowland tropical rainforest has been converted in large into plantation systems. Despite the evident changes above ground, the effect of rainforest conversion on the channeling of energy in soil food webs was not studied. Here, we investigated community‐level neutral lipid fatty acid profiles in dominant soil fauna to track energy channels in rainforest, rubber, and oil palm plantations in Sumatra, Indonesia. Abundant macrofauna including Araneae, Chilopoda, and Diplopoda contained high amounts of plant and fungal biomarker fatty acids (FAs). Lumbricina had the lowest amount of plant, but the highest amount of animal‐synthesized C20 polyunsaturated FAs as compared to other soil taxa. Mesofauna detritivores (Collembola and Oribatida) contained high amounts of algal biomarker FAs. The differences in FA profiles between taxa were evident if data were analyzed across land‐use systems, suggesting that soil fauna of different size (macro‐ and mesofauna) are associated with different energy channels. Despite that, rainforest conversion changed the biomarker FA composition of soil fauna at the community level. Conversion of rainforest into oil palm plantations enhanced the plant energy channel in soil food webs and reduced the bacterial energy channel; conversion into rubber plantations reduced the AMF‐based energy channel. The changes in energy distribution within soil food webs may have significant implications for the functioning of tropical ecosystems and their response to environmental changes. At present, these responses are hard to predict considering the poor knowledge on structure and functioning of tropical soil food webs. 相似文献
103.
104.
S. S. Mikhailovsky A. M. Kulikov S. G. Potapov O. E. Lazebny V. G. Mitrofanov 《Russian Journal of Genetics》2007,43(1):88-91
A comparative analysis of the sibling species of Drosophila virilis was performed by RAPD-PCR technique using a set of random primers. The degree of relatedness was studied by cluster analysis (UPGMA) and multidimensional scaling. The resulting pattern of species relationships contradicts the classical taxonomy. The main result of the cluster analysis is that D. virilis does not cluster with the remaining three species of its phylad, while according to multidimensional scaling, D. virilis is equidistant from all the species of its group, from both the species of its phylad and the species of the montana phylad. The montana phylad is extremely heterogeneous; moreover, the species D. littoralis, D. ezoana, and D. kanekoi appear to be closer to the virilis phylad than to the other species of the montana phylad, wherein these species are traditionally included. The phylogenetic relationships between the studied species discovered using RAPD fingerprinting comply with the results obtained using protein markers and quantitative traits. 相似文献
105.
Aaron L. Berkovsky Pavel P. Potapov 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1997,692(2):273
Placental protein 14 was isolated from the biological material of patients undergoing legal abortions. The major part of ballast protein was removed by ion-exchange chromatography on DEAE-Sepharose and CM-Sepharose. Albumin was separated by chromatography on Blue-Sepharose. Complete purification was obtained by metal-chelate affinity chromatography on Nickel-Chelate Sepharose and hydrophobic interaction chromatography on Phenyl-Sepharose and Octyl-Sepharose. The protein was not exposed to denaturing agents or extreme pH. 相似文献
106.
E. V. Sharova V. G. Amcheslavskii A. A. Potapov V. L. Anzimirov O. S. Zaitsev V. K. Emel'yanov V. A. Shabalov 《Human physiology》2001,27(2):155-164
The effects of therapeutic SCS and transcranial electric stimulation on the functional activity of the brain in seven patients in the posttraumatic unconscious state were compared. The therapeutic transcranial stimulation was shown to exert a positive effect on the EEG and the characteristics of its spatial–temporal organization in most cases, which corresponds with positive shifts in the mental and motor sphere. The phasic character of changes in the bioelectric activity reflecting the sequence of involvement of the cerebral structures (primary activation of the deep frontal lobe divisions, activation of the subcortical-diencephalic structures, activation of the cortex) in the developing adaptive reactions, which helps understand their neurophysiological mechanisms, was described. It was established that the formation of the foci of persistent pathological activity with dominant characteristics in the brain is one of the possible complications of both SCS and transcranial electric stimulation. The mechanisms of their neurogenesis and the electrographic equivalents require further study. The data may serve as the basis for further clinical study of the influence of transcranial electric stimulation on the recovery of patients in postcomatose unconscious states. 相似文献
107.
Daniel J. Zarin Nancy L. Harris Alessandro Baccini Dmitry Aksenov Matthew C. Hansen Claudia Azevedo‐Ramos Tasso Azevedo Belinda A. Margono Ane C. Alencar Chris Gabris Adrienne Allegretti Peter Potapov Mary Farina Wayne S. Walker Varada S. Shevade Tatiana V. Loboda Svetlana Turubanova Alexandra Tyukavina 《Global Change Biology》2016,22(4):1336-1347
Halving carbon emissions from tropical deforestation by 2020 could help bring the international community closer to the agreed goal of <2 degree increase in global average temperature change and is consistent with a target set last year by the governments, corporations, indigenous peoples' organizations and non‐governmental organizations that signed the New York Declaration on Forests (NYDF). We assemble and refine a robust dataset to establish a 2001–2013 benchmark for average annual carbon emissions from gross tropical deforestation at 2.270 Gt CO2 yr?1. Brazil did not sign the NYDF, yet from 2001 to 2013, Brazil ranks first for both carbon emissions from gross tropical deforestation and reductions in those emissions – its share of the total declined from a peak of 69% in 2003 to a low of 20% in 2012. Indonesia, an NYDF signatory, is the second highest emitter, peaking in 2012 at 0.362 Gt CO2 yr?1 before declining to 0.205 Gt CO2 yr?1 in 2013. The other 14 NYDF tropical country signatories were responsible for a combined average of 0.317 Gt CO2 yr?1, while the other 86 tropical country non‐signatories were responsible for a combined average of 0.688 Gt CO2 yr?1. We outline two scenarios for achieving the 50% emission reduction target by 2020, both emphasizing the critical role of Brazil and the need to reverse the trends of increasing carbon emissions from gross tropical deforestation in many other tropical countries that, from 2001 to 2013, have largely offset Brazil's reductions. Achieving the target will therefore be challenging, even though it is in the self‐interest of the international community. Conserving rather than cutting down tropical forests requires shifting economic development away from a dependence on natural resource depletion toward recognition of the dependence of human societies on the natural capital that tropical forests represent and the goods and services they provide. 相似文献
108.
The variability of the genomes of ten vole species was analyzed by means of taxonomic DNA fingerprinting and polymerase chain reaction using random primers (RAPD-PCR). The dendrograms of genetic similarity between the representatives of the tribe Arvicolinae (Gray, 1821) were constructed, based on the data obtained by means of both methods. The topology of the genetic similarity dendrogram that is based on the RAPD-PCR data generally correlates with the genetic distances estimated from biochemical and karyological data. The results did not confirm the genus status of Terricola. On the other hand, the data from taxonprint analysis suggest the recognition of Lasiopodomys as a separate genus. 相似文献
109.
A chelate cationic rhodium(I) complex with a hemilabile amino- and sulfur-containing phosphinite ligand has been synthesized and, according to the NMR data, the ligand is bound to the metal in a P,S-bidentate coordination mode without any Rh-N interaction. This complex efficiently catalyzes the hydroformylation of styrene. The chelate rhodium complex with the analogous ligand without the amino group has also been synthesized and examined as a catalyst for the same hydroformylation reaction. The reaction rate is higher using the former complex compared to the latter one without the amino group, with, however, a slightly lower regioselectivity towards the formation of the branched aldehyde. 相似文献
110.
We develop a model of CWD management by nonselective deer harvest, currently the most feasible approach available for managing CWD in wild populations. We use the model to explore the effects of 6 common harvest strategies on disease prevalence and to identify potential optimal harvest policies for reducing disease prevalence without population collapse. The model includes 4 deer categories (juveniles, adult females, younger adult males, older adult males) that may be harvested at different rates, a food-based carrying capacity, which influences juvenile survival but not adult reproduction or survival, and seasonal force of infection terms for each deer category under differing frequency-dependent transmission dynamics resulting from environmental and direct contact mechanisms. Numerical experiments show that the interval of transmission coefficients β where the disease can be controlled is generally narrow and efficiency of a harvest policy to reduce disease prevalence depends crucially on the details of the disease transmission mechanism, in particular on the intensity of disease transmission to juveniles and the potential differences in the behavior of older and younger males that influence contact rates. Optimal harvest policy to minimize disease prevalence for each of the assumed transmission mechanisms is shown to depend on harvest intensity. Across mechanisms, a harvest that focuses on antlered deer, without distinguishing between age classes reduces disease prevalence most consistently, whereas distinguishing between young and older antlered deer produces higher uncertainty in the harvest effects on disease prevalence. Our results show that, despite uncertainties, a modelling approach can determine classes of harvest strategy that are most likely to be effective in combatting CWD. 相似文献