乙肝患者肝纤维化血清标志物表达水平及其临床意义

目的探讨乙肝患者肝纤维化血清标志物表达水平及其与病情、病程的关系。方法用R IA方法对135例乙肝患者进行血清透明质酸酶(HA)、层粘连蛋白(LN)、Ⅲ型胶原前体(PⅢC)、Ⅳ型胶原(ⅣC)检测。结果乙肝患者血清中HA、LN、PⅢC和ⅣC水平分别为(338.7±122.6)、(226.2±43.3)、(152.8±33.7)和(132.6±41.1)μg/L。其中,急性黄疸肝炎、慢性肝炎轻度、慢性肝炎中度、慢性肝炎重度、肝硬变代偿期、肝硬变失代偿期、肝癌和肝硬变+肝癌患者HA、LN、PⅢC和ⅣC水平分别为(134.2±101.4)、(185.1±104.5)、(373.5±147.1)、(445.5±123.6)、(477.3±186.0)、(653.7±98.5)、(597.5±91.3)和(723.6±110.4)μg/L;(106.7±31.3)、(179.5±28.5)、(234.6±66.1)、(287.3±57.0)、(294.8±66.4)、(345.6±61.2)、(322.1±71.0)和(357.8±88.3)μg/L;(103.5±19.2)、(108.6±21.4)、(145.3±37.3)、(194.5±41.7)、(188.8±39.5)、(247.6±48.0)、(251.3±37.3)和(286.7±35.8)μg/L;(49.6±14.7)、(83.4±15.3)、(134.5±50.5)、(178.7±61.4)、(183.2±61.3)、(256.8±72.4)、(217.7±64.5)和(281.1±57.0)μg/L。结论肝癌、肝硬化、肝硬变+肝癌和慢性肝炎重度患者存在明显的肝纤维化征象,急性黄疸肝炎、慢性肝炎轻度患者肝纤维化程度较轻,在一定范围内肝纤维化程度与肝病的病情、病程关系密切。     

Isolation and characterization of polymorphic microsatellite loci from the pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae)

Thirteen polymorphic microsatellite markers were developed from an enriched partial genomic library for the pink bollworm, Pectinophora gossypiella (Saunders), one of the most important cotton pests in the world. The total number of alleles ranged from two to 12 for a sample of 50 individuals and the expected heterozygosity at these loci ranged from 0.042 to 0.830. Although the presence of null alleles in some loci is suspected, these polymorphic markers are likely to provide useful tools for the population genetic studies of this species.