全文获取类型
收费全文 | 601篇 |
免费 | 29篇 |
出版年
2023年 | 3篇 |
2022年 | 15篇 |
2021年 | 23篇 |
2020年 | 12篇 |
2019年 | 13篇 |
2018年 | 21篇 |
2017年 | 21篇 |
2016年 | 24篇 |
2015年 | 30篇 |
2014年 | 35篇 |
2013年 | 39篇 |
2012年 | 55篇 |
2011年 | 40篇 |
2010年 | 21篇 |
2009年 | 11篇 |
2008年 | 21篇 |
2007年 | 24篇 |
2006年 | 28篇 |
2005年 | 23篇 |
2004年 | 22篇 |
2003年 | 14篇 |
2002年 | 19篇 |
2001年 | 4篇 |
2000年 | 6篇 |
1999年 | 4篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 5篇 |
1988年 | 8篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1984年 | 8篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1979年 | 2篇 |
1977年 | 4篇 |
1974年 | 4篇 |
1969年 | 3篇 |
1959年 | 2篇 |
1958年 | 2篇 |
1957年 | 4篇 |
1956年 | 2篇 |
排序方式: 共有630条查询结果,搜索用时 781 毫秒
101.
Seasonal dynamics of zooplankton in a shallow eutrophic,man-made hyposaline lake in Delhi (India): role of environmental factors 总被引:1,自引:0,他引:1
Old Fort Lake, a small (1.6 ha), shallow, and recreational water body in Delhi (India) was studied through monthly surveys
in two consecutive years (January, 2000–December, 2001). Precipitation is the major source of water for this closed basin
lake. In addition, ground water is used for replenishing the lake regularly. This alkaline, hyposaline hard water lake contains
very high ionic concentration, especially of nitrates. Based on overall ionic composition, this lake can be categorized as
chloride–sulfate alkaline waters with the anion sequence dominated by SO4
2− > Cl− > HCO3
−, and the cations by Mg++ > Ca++. The overall seasonal variability in physicochemical profile was largely regulated by the annual cycle of evaporation and
precipitation, whereas the ground water largely influences its water quality. The lake exhibited phytoplankton-dominated turbid
state due to dominance of the blue green alga, Microcystis aeruginosa. The persistent cyanobacterial blooms and the elevated nutrient levels are indicative of the cultural eutrophication of the
lake. This study focuses on the relative importance of eutrophic vis-à-vis hyposaline conditions in determining the structure
and seasonal dynamics of zooplankton species assemblages. A total of 52 zooplankton species were recorded and rotifers dominated
the community structure qualitatively as well as quantitatively. The genus Brachionus comprised a significant component of zooplankton community with B. plicatilis as the most dominant species. The other common taxa were B. quadridentatus, B. angularis, Lecane grandis, L. thalera, L. punctata, Mesocyclops sp., and Alona rectangula. Multivariate data analysis techniques, Canonical Correspondence Analysis (CCA) along with Monte Carlo Permutation Tests
were used to determine the minimum number of environmental factors that could explain statistically significant (P < 0.05) proportions of variation in the species data. The significant variables selected by CCA were NH3–N followed by percent saturation of DO, COD, SS, BOD, NO2–N, rainfall, silicates, and PO4–P. The results indicate that the seasonal succession patterns of the zooplankton species were largely controlled by physicochemical
factors related directly or indirectly to the process of eutrophication, whereas hyposaline conditions in the lake determined
the characteristic species composition.
Guest Editors: J. John & B. Timms
Salt Lake Research: Biodiversity and Conservation—Selected papers from the 9th Conference of the International Society for
Salt Lake Research 相似文献
102.
103.
Background
Relapsing fever (RF) spirochetes are notable for multiphasic antigenic variation of polymorphic outer membrane lipoproteins, a phenomenon responsible for immune evasion. An additional role in tissue localization is suggested by the finding that isogenic serotypes 1 (Bt1) and 2 (Bt2) of the RF spirochete Borrelia turicatae, which differ only in the Vsp they express, exhibit marked differences in clinical disease severity and tissue localization during infection.Methodology/Principal Findings
Here we used known vsp DNA sequences encoding for B. turicatae and Borrelia hermsii Vsp proteins with variable regions and then studied whether there are differences in disease expression and tissue localization of their corresponding serotypes during mouse infection. For sequence and structural comparisons we focused exclusively on amino acid residues predicted to project away from the spirochetes surface, referred to as the Vsp dome. Disease severity and tissue localization were studied during persistent infection with individual or mixed serotypes in SCID mice. The results showed that all Vsp domes clustered into 3 main trunks, with the domes for B. turicatae Vsp1 (BtVsp1) and BtVsp2 clustering into separate ones. B. hermsii serotypes whose Vsp domes clustered with the BtVsp1 dome were less virulent but localized to the brain more. The BtVsp2 dome was the oddball among all and Bt2 was the only serotype that caused severe arthritis.Conclusion/Significance
These findings indicate that there is significant variability in Vsp dome structure, disease severity, and tissue localization among serotypes of B. hermsii. 相似文献104.
105.
Background
Novel therapeutics are urgently needed to control tuberculosis (TB). Thioridazine (THZ) is a candidate for the therapy of multidrug and extensively drug-resistant TB.Methodology/Principal Findings
We studied the impact of THZ on Mycobacterium tuberculosis (Mtb) by analyzing gene expression profiles after treatment at the minimal inhibitory (1x MIC) or highly inhibitory (4x MIC) concentrations between 1–6 hours. THZ modulated the expression of genes encoding membrane proteins, efflux pumps, oxido-reductases and enzymes involved in fatty acid metabolism and aerobic respiration. The Rv3160c-Rv3161c operon, a multi-drug transporter and the Rv3614c/3615c/3616c regulon, were highly induced in response to THZ. A significantly high number of Mtb genes co-expressed with σB (the σB regulon) was turned on by THZ treatment. σB has recently been shown to protect Mtb from envelope-damage. We hypothesized that THZ damages the Mtb cell-envelope, turning on the expression of the σB regulon. Consistent with this hypothesis, we present electron-microscopy data which shows that THZ modulates cell-envelope integrity. Moreover, the Mtb mutants in σH and σE, two alternate stress response sigma factors that induce the expression of σB, exhibited higher sensitivity to THZ, indicating that the presence and expression of σB allows Mtb to resist the impact of THZ. Conditional induction of σB levels increased the survival of Mtb in the presence of THZ.Conclusions/Significance
THZ targets different pathways and can thus be used as a multi-target inhibitor itself as well as provide strategies for multi-target drug development for combination chemotherapy. Our results show that the Mtb sigma factor network comprising of σH, σE and σB plays a crucial role in protecting the pathogen against cell-envelope damage. 相似文献106.
Latonya F. Been Swapan K. Nath Sarju K. Ralhan Gurpreet S. Wander Narinder K. Mehra Jairup Singh John J. Mulvihill Dharambir K. Sanghera 《Obesity (Silver Spring, Md.)》2010,18(2):425-429
Recent genome‐wide association studies (GWAS) in Asian Indians reported strong associations of variants near melanocortin‐4 receptor (MC4R) and MLX interacting protein‐like (MLXIPL) genes with insulin resistance and several obesity‐related quantitative traits (QTs). Here, we evaluated the association of two variants (rs12970134 and rs4450508) near MC4R and a nonsynonymous (Gln241His) variant (rs3812316) in MLXIPL gene with type 2 diabetes (T2D) and obesity‐related QTs in our case–control cohort (n = 1,528; 745 T2D cases and 783 controls) from a Sikh population from North India. We have successfully replicated the association of MC4R (rs12970134) with BMI (P = 0.0005), total weight (WT) (P = 0.001), and waist circumference (WC) (P = 0.001). These associations remained significant after controlling for multiple testing by applying Bonferroni's correction. However, our data did not confirm the association of rs3812316 in the MLXIPL gene with triglyceride (TG) levels. These observations demonstrate that the genetic variation in MC4R locus can have a moderate contribution in the regional fat deposition and development of central obesity in Asian Indians. 相似文献
107.
108.
109.
Poonam Gusain Rashmi Paliwal Vir Singh 《International journal of phytoremediation》2017,19(3):290-299
In search of multitrait plant growth–promoting (PGP) inoculants, we introduced two cadmium-resistant bacterial strains, C4 (PG), C5 (WB), and their consortium C6 (PG × WB) isolated from metal-contaminated industrial waste–fed canal near West Bengal. The test isolates were biochemically characterized and screened in vitro for siderophore production. The infrared spectra revealed the hydroxamate nature of the siderophore produced. Further in green house, siderophore-based seed inoculation with selected PGP isolates exhibited stimulatory effects on seed germination (up to 85.4%), chlorophyll index (22.9 spad unit), shoot and root length (70% and 62.7%), tiller numbers (38.82%), spikelet numbers (52.2%), straw yield (62.2%), grain yield (76.1%), total dry matter of root and shoot (55.56% and 64.4%, respectively), and grain yields (76.1%) of tested wheat cultivars. The 16S rRNA sequencing identified strain PG and WB as Dietzia maris and Lysinibacillus sp. strains. Furthermore, inoculation of C6 (consortium) in both cultivar UP-2565 and KS-227 showed maximum Cd sorption capacity in roots (38.3% and 67.1%) and shoots (68.4% and 67.5%), respectively. Both the strains and their consortium showed a great potential to increase the growth and yield of wheat cultivars, which can also be utilized for rhizoremediation process. 相似文献
110.