排序方式: 共有506条查询结果,搜索用时 375 毫秒
431.
Nwe Nwe Oo Germana Bancone Lwin Zar Maw Nongnud Chowwiwat Pooja Bansil Gonzalo J. Domingo Moh Moh Htun Kyaw Zin Thant Ye Htut Francois Nosten 《PloS one》2016,11(4)
Primaquine and other 8-amnoquinoline based anti-malarials can cause haemolysis in subjects with glucose-6-phosphate dehydrogenase (G6PD) deficiency. Correct diagnosis of G6PD status in patients is crucial for safe treatment of both relapsing stages of Plasmodium vivax and transmitting forms of Plasmodium falciparum. Lack of suitable point-of-care tests has hampered a much needed wide use of primaquine for malaria elimination. In this study we have assessed the performances of two qualitative tests, the fluorescent spot test (FST) and the G6PD CareStart test (CST), against the gold standard quantitative spectrophotometric assay in a population of 1000 random adult healthy volunteers living in Yangon, Myanmar. The prevalence of G6PD deficiency in the Bamar, Karen and in the whole sample set was 6.6% (10.1% in males), 9.2% (21.0% in males) and 6.8% (11.1% in males) respectively. The FST and CST showed comparable performances with sensitivity over 95% and specificity over 90%, however for cases with severe G6PD activity the FTS had improved performance. If used with a conservative interpretation of the signal, the CareStart test has the potential to be used in the field and, by allowing a wider use of primaquine, to help malaria elimination. 相似文献
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RGS proteins accelerate the GTPase activity of heterotrimeric G proteins at the plasma membrane. Association of RGS proteins with the plasma membrane can be mediated by interactions with other membrane proteins and by direct interactions with the lipid bilayer. Here we use fluorescence recovery after photobleaching (FRAP) to characterize interactions between RGS2 and M3 acetylcholine receptors (M3Rs), Galpha subunits and the lipid bilayer. Active Galpha(q) and M3Rs both recruited RGS2-EGFP to the plasma membrane. RGS2-EGFP remained bound to the plasma membrane between interactions with active Galpha(q), but rapidly exchanged between membrane-associated and cytosolic pools when recruited by M3Rs. 相似文献
435.
Dhanendra Tomar Fabián Jaña Zhiwei Dong William J. Quinn Pooja Jadiya Sarah L. Breves Cassidy C. Daw Subramanya Srikantan Santhanam Shanmughapriya Neeharika Nemani Edmund Carvalho Aparna Tripathi Alison M. Worth Xueqian Zhang Roshanak Razmpour Ajay Seelam Stephen Rhode Anuj V. Mehta Muniswamy Madesh 《Cell reports》2019,26(13):3709-3725.e7
436.
Vijaykumar Deore Nilambari Yewalkar Dimple Bhatia Nikesh Desai Ravindra D. Gupte Shruta S. Dadarkar Mahesh G. Jadhav Aditi A. Tannu Pooja Bhatt Kumar V.S. Nemmani Ram A. Vishwakarma Somesh Sharma Abhijit Roychowdhury Nilesh M. Dagia Mandar R. Bhonde Sanjay Kumar 《Bioorganic & medicinal chemistry letters》2009,19(11):2949-2952
A series of novel cyanopyridyl based molecules (1–14) were designed, synthesized and probed for inhibition of mammalian target of rapamycin (mTOR) activity. Compound 14 was found to be a potent inhibitor of mTOR activity as assessed by enzyme-linked immunoassays and Western blot analysis. Most importantly, systemic application (intraperitoneal; ip) of compound 14 significantly suppressed macroscopic and histological abnormalities associated with chemically-induced murine colitis. 相似文献
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Pooja Bhatnagar-Mathur Vincent Vadez M. Jyostna Devi M. Lavanya G. Vani Kiran K. Sharma 《Molecular breeding : new strategies in plant improvement》2009,23(4):591-606
Abiotic stresses including water deficit severely limits crop yields in the semi-arid tropics. In chickpea, annual losses
of over 3.7 million tones have been estimated to be due to water deficit conditions alone. Therefore, major efforts are needed
to improve its tolerance to water deficit, and genetic engineering approaches provide an increasing hope for this possibility.
We have used transgenic technology for the introduction of an osmoregulatory gene P5CSF129A encoding the mutagenized Δ1-pyrroline-5-carboxylate synthetase (P5CS) for the overproduction of proline. A total of 49 transgenic events of chickpea were produced with the 35S:P5CSF129A gene
through Agrobacterium tumefaciens-mediated gene transfer through the use of axillary meristem explants. Eleven transgenic events that accumulated high proline
(2–6 folds) were further evaluated in greenhouse experiments based on their transpiration efficiency (TE), photosynthetic
activity, stomatal conductance, and root length under water stress. Almost all the transgenic events showed a decline in transpiration
at lower values of the fraction of transpirable soil water (dryer soil), and extracted more water than their untransformed
parents. The accumulation of proline in the selected events was more pronounced that increased significantly in the leaves
when exposed to water stress. However, the overexpression of P5CSF129A gene resulted only in a modest increase in TE, thereby indicating that the enhanced proline had little bearing on the components
of yield architecture that are significant in overcoming the negative effects of drought stress in chickpea. 相似文献
439.
Graziella Mendonsa Justyna Dobrowolska Angela Lin Pooja Vijairania Y.-J. I. Jong Nancy L. Baenziger 《PloS one》2009,4(2)
The serious and growing impact of the neurodegenerative disorder Alzheimer''s disease (AD) as an individual and societal burden raises a number of key questions: Can a blanket test for Alzheimer''s disease be devised forecasting long-term risk for acquiring this disorder? Can a unified therapy be devised to forestall the development of AD as well as improve the lot of present sufferers? Inflammatory and oxidative stresses are associated with enhanced risk for AD. Can an AD molecular signature be identified in signaling pathways for communication within and among cells during inflammatory and oxidative stress, suggesting possible biomarkers and therapeutic avenues? We postulated a unique molecular signature of dysfunctional activity profiles in AD-relevant signaling pathways in peripheral tissues, based on a gain of function in G-protein-coupled bradykinin B2 receptor (BKB2R) inflammatory stress signaling in skin fibroblasts from AD patients that results in tau protein Ser hyperphosphorylation. Such a signaling profile, routed through both phosphorylation and proteolytic cascades activated by inflammatory and oxidative stresses in highly penetrant familial monogenic forms of AD, could be informative for pathogenesis of the complex multigenic sporadic form of AD. Comparing stimulus-specific cascades of signal transduction revealed a striking diversity of molecular signaling profiles in AD human skin fibroblasts that express endogenous levels of mutant presenilins PS-1 or PS-2 or the Trisomy 21 proteome. AD fibroblasts bearing the PS-1 M146L mutation associated with highly aggressive AD displayed persistent BKB2R signaling plus decreased ERK activation by BK, correctible by gamma-secretase inhibitor Compound E. Lack of these effects in the homologous PS-2 mutant cells indicates specificity of presenilin gamma-secretase catalytic components in BK signaling biology directed toward MAPK activation. Oxidative stress revealed a JNK-dependent survival pathway in normal fibroblasts lost in PS-1 M146L fibroblasts. Complex molecular profiles of signaling dysfunction in the most putatively straightforward human cellular models of AD suggest that risk ascertainment and therapeutic interventions in AD as a whole will likely demand complex solutions. 相似文献
440.
Pearce MJ Arora P Festa RA Butler-Wu SM Gokhale RS Darwin KH 《The EMBO journal》2006,25(22):5423-5432
The putative proteasome-associated proteins Mpa (Mycobaterium proteasomal ATPase) and PafA (proteasome accessory factor A) of the human pathogen Mycobacterium tuberculosis (Mtb) are essential for virulence and resistance to nitric oxide. However, a direct link between the proteasome protease and Mpa or PafA has never been demonstrated. Furthermore, protein degradation by bacterial proteasomes in vitro has not been accomplished, possibly due to the failure to find natural degradation substrates or other necessary proteasome co-factors. In this work, we identify the first bacterial proteasome substrates, malonyl Co-A acyl carrier protein transacylase and ketopantoate hydroxymethyltransferase, enzymes that are required for the biosynthesis of fatty acids and polyketides that are essential for the pathogenesis of Mtb. Maintenance of the physiological levels of these enzymes required Mpa and PafA in addition to proteasome protease activity. Mpa levels were also regulated in a proteasome-dependent manner. Finally, we found that a conserved tyrosine of Mpa was essential for function. Thus, these results suggest that Mpa, PafA, and the Mtb proteasome degrade bacterial proteins that are important for virulence in mice. 相似文献