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61.
Chemical cross-linking of gelatin is achieved using a thermosensitive reactive copolymer based on N-isopropylacrylamide (NIPAM). The copolymer bears 5 mol % acrylic acid units which form amide bonds with the amino groups of gelatin in the presence of a water-soluble carbodiimide. The cross-linking reaction occurs only below the LCST congruent with 34 degrees C (lower critical solution temperature), i.e., when the copolymer is in the coil conformation. Above the LCST the copolymer adopts a globule conformation and its ability to react with gelatin is drastically reduced. By setting the temperature above or below the LCST it is possible to switch off or on the reactivity of the system and control the gelation process. The switch temperature can be set at the desired value by adjusting the composition of the thermosensitive copolymer. 相似文献
62.
Emonds-Alt X Proietto V Steinberg R Advenier C Daoui S Naline E Gueudet C Michaud JC Oury-Donat F Poncelet M Vilain P Le Fur G Maffrand JP Soubrié P Pascal M 《Canadian journal of physiology and pharmacology》2002,80(5):482-488
SSR 146977 is a potent and selective antagonist of the tachykinin NK3 receptor. In Chinese hamster ovary cells expressing the human tachykinin NK3 receptor, SSR 146977 inhibited the binding of radioactive neurokinin B to NK3 receptors (Ki = 0.26 nM), senktide (10 nM) induced inositol monophosphate formation (IC50 = 7.8-13 nM), and intracellular calcium mobilization (IC50 = 10 nM). It antagonized [MePhe7]neurokinin B induced contractions of guinea pig ileum (pA2 = 9.07). Senktide (30 nM) induced firing rate increase of noradrenergic neurons in the guinea pig locus coeruleus and dopaminergic neurons in the guinea pig substantia nigra was also blocked by SSR 146977 (50 and 100 nM, respectively). In vivo, in the respiratory system, SSR 146977 inhibited bronchial hyperresponsiveness to acetylcholine, bronchial microvascular permeability hypersensitivity to histamine (doses of 0.1-1 mg/kg i.p.), and cough (doses of 0.03-1 mg/kg i.p.) provoked by citric acid in guinea pigs. In the central nervous system, SSR 146977 inhibited turning behaviour (ID50 = 0.2 mg/kg i.p. and 0.4 mg/kg p.o.) and prevented the decrease of locomotor activity (10 and 30 mg/kg i.p) mediated by the stimulation of NK3 receptors in gerbils. In guinea pigs, SSR 146977 antagonized senktide-induced acetylcholine release in the hippocampus (0.3 and 1 mg/kg i.p) and norepinephrine release in the prefrontal cortex (0.3 mg/kg i.p.). It also prevented haloperidol-induced increase of the number of spontaneously active dopamine A10 neurons (1 and 3 mg/kg i.p.). 相似文献
63.
Modulating skeletal muscle mass by postnatal,muscle-specific inactivation of the myostatin gene 总被引:1,自引:0,他引:1
Grobet L Pirottin D Farnir F Poncelet D Royo LJ Brouwers B Christians E Desmecht D Coignoul F Kahn R Georges M 《Genesis (New York, N.Y. : 2000)》2003,35(4):227-238
By using a conditional gene targeting approach exploiting the cre-lox system, we show that postnatal inactivation of the myostatin gene in striated muscle is sufficient to cause a generalized muscular hypertrophy of the same magnitude as that observed for constitutive myostatin knockout mice. This formally demonstrates that striated muscle is the production site of functional myostatin and that this member of the TGFbeta family of growth and differentiation factors regulates muscle mass not only during early embryogenesis but throughout development. It indicates that myostatin antagonist could be used to treat muscle wasting and to promote muscle growth in man and animals. 相似文献
64.
Improved performances and control of beer fermentation using encapsulated alpha-acetolactate decarboxylase and modeling 总被引:1,自引:0,他引:1
The use of the enzyme alpha-acetolactate decarboxylase allows the acceleration of beer fermentation/maturation because it shunts diacetyl formation, whose elimination is the rate-limiting step of the process. To obtain a cost reduction by using this exogenous enzyme, we propose a new process involving recoverable encapsulated alpha-acetolactate decarboxylase. The performance of traditional and new processes was investigated by a modeling approach. A simple model, focused on alpha-acetolactate and diacetyl profiles during beer fermentation, was set up. The simulated profiles are consistent with literature data. This study shows also that encapsulated alpha-acetolactate decarboxylase allows the acceleration of beer fermentation as efficiently as free alpha-acetolactate decarboxylase. The advantage of immobilized alpha-acetolactate decarboxylase versus free enzyme is that it is recoverable and reusable, which means a process cost reduction. 相似文献
65.
Patrick Angibaud Kristof Van Emelen Laurence Decrane Sven van Brandt Peter ten Holte Isabelle Pilatte Bruno Roux Virginie Poncelet David Speybrouck Laurence Queguiner Sandrine Gaurrand Ann Mariën Wim Floren Lut Janssen Marc Verdonck Jacky van Dun Jacky van Gompel Ron Gilissen Claire Mackie Marc Du Jardin Janine Arts 《Bioorganic & medicinal chemistry letters》2010,20(1):294-298
Pursuing our efforts in designing 5-pyrimidylhydroxamic acid anti-cancer agents, we have identified a new series of potent histone deacetylase (HDAC) inhibitors. These compounds exhibit enzymatic HDAC inhibiting properties with IC50 values in the nanomolar range and inhibit tumor cell proliferation at similar levels. Good solubility, moderate bioavailability, and promising in vivo activity in xenograft model made this series of compounds interesting starting points to design new potent HDAC inhibitors. 相似文献
66.
AbstractSuperoxide radical represents one of the most biologically relevant reactive oxygen species involved in numerous physiological and pathophysiological processes. Superoxide measurement through the decay of an electron paramagnetic resonance (EPR) signal of a triarylmethyl (TAM) radical possesses the advantage of a high selectivity and relatively high rate constant of TAM reaction with the superoxide. Hereby we report a straightforward synthesis and characterization of a TAM–TAM biradical showing a high reactivity with superoxide (second-order rate constant, (6.7?±?0.2)?×?103 M?1 s?1) enabling the measurement of superoxide radical by following the increase of a sharp EPR signal associated with the formation of a TAM-quinone-methide monoradical product. 相似文献
67.
Emmanuel R Marciano L Polliand C Antonio V Ziol M Poncelet C Barrat C 《Diagnostic pathology》2012,7(1):62
ABSTRACT: Cecal endometriosis and ileocolic intussusception due to a cecal endometriosis is extremely rare. We report a case of a woman who presented an ileocecal intussusception due to a cecal endometriosis. The patient gave two months history of chronic periombilical pain requiring regular hospital admission and analgesia. The symptoms were not related to menses. A laparotomy was performed and revealed an ileocolic intussusception. The abdominal exploration did not find any endometriosis lesion. Ileocaecal resection was performed. Microscopic examination showed a cystic component, lined by a regular cylindric epithelium. Foci of endometrial tissu were oberved in the cecal subserosa and muscularis mucosal, with irregular endometrial glands lined by cylindric epithelium without atypia immunostained with CK7, and characteristic endometrial stroma immunostained with CD10. Cecal endometriosis and ileocolic intussusception due to a cecal endometriosis is extremely rare. Diagnose of etiology remains challenging due to the absence of clinical and radiological specific characteristics. Virtual slide The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2975867306869166. 相似文献
68.
Peter Verhasselt Filip Poncelet Karel Vits August Van Gool Jos Vanderleyden 《FEMS microbiology letters》1989,59(1-2):135-140
A gene library of Clostridium acetobutylicum ATCC824 was constructed in the plasmid vector pEcoR251. The library was tested for the presence of starch hydrolyzing clones. One clone in which the recombinant plasmid, pVP101, conferred alpha-amylase activity to the Escherichia coli host cell, was detected. The gene is carried on a 3.45-kbp BglII restriction fragment. A detailed physical map of pVP101 is presented. 相似文献
69.
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