全文获取类型
收费全文 | 124篇 |
免费 | 4篇 |
出版年
2022年 | 1篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 6篇 |
2014年 | 5篇 |
2013年 | 11篇 |
2012年 | 8篇 |
2011年 | 15篇 |
2010年 | 3篇 |
2009年 | 5篇 |
2008年 | 8篇 |
2007年 | 5篇 |
2006年 | 8篇 |
2005年 | 4篇 |
2004年 | 2篇 |
2003年 | 2篇 |
2002年 | 4篇 |
2001年 | 1篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 4篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有128条查询结果,搜索用时 0 毫秒
121.
122.
Knowledge of inter and intra-specific variation in the susceptibility of natural enemies to pesticides could help to better design integrated pest management strategies. The objective of this research was to evaluate the susceptibility to deltamethrin in populations of the predatory mites Neoseiulus californicus (McGregor) and Phytoseiulus macropilis (Banks) populations collected from protected ornamental crops in Brazil. The susceptibility to deltamethrin was characterized against immature and adult stages of both species. The impact of this insecticide was also measured by estimating the intrinsic rate of increase (r i). The immature and adult stages of N. californicus were approximately 3,600 and 3,000-fold more tolerant to deltamethrin than those of P. macropilis. However, high variability in the susceptibility to this insecticide was detected among P. macropilis populations, with resistance ratios of up to 3,500-fold. The selection of deltamethrin-resistant strains of P. macropilis could be exploited in applied biological control programs. 相似文献
123.
124.
A Poletti C Giacon N Pennelli 《The journal of histochemistry and cytochemistry》1992,40(12):1965-1970
We explored the possibility of simultaneous application of histochemical and immunohistochemical staining techniques on the same paraffin-embedded human tissue section. Conventional histological stains (PAS, Alcian, Alcian-PAS, Van Gieson, Gomori silver impregnation, and Giemsa) were used in association with a battery of markers (keratins, leucocyte common antigen, S-100 protein, Factor VIII-related antigen) that are widely employed in diagnostic and experimental studies. We found that the best procedure was to perform immunostaining before the histochemical reaction, as this enables all the other possible combinations to be carried out. In addition, several detection systems, such as peroxidase-anti-peroxidase (PAP), alkaline phosphatase-anti-alkaline phosphatase (APAAP), and avidin-biotin complex (ABC), were tested and all gave consistent results. Some minor modifications of the histological staining methods were necessary, but the current immunohistochemical techniques could be used as established. Preliminary findings indicate that immunohistochemistry can be combined with histochemistry techniques by means of a relatively simple procedure whose only disadvantage is the time required to carry out the double staining. 相似文献
125.
126.
127.
R. Ridolfi Emanuela Flamini Angela Riccobon F. De Paola Roberta Maltoni A. Gardini Laura Ridolfi Laura Medri Giovanni Poletti Dino Amadori 《Cancer immunology, immunotherapy : CII》1998,46(4):185-193
Adoptive tumour infiltrating lymphocytes (TIL) in combination with a modulated dosage of interleukin-2 (IL-2) can be used
with acceptable toxicity in the treatment of immunogenic tumours. Following an experience of reinfusion in advanced melanoma,
colorectal and renal cancer patients, treatment was given to disease-free patients after metastasectomy. The high risk of
relapse and favourable ratio between reinfused TIL and possible microscopic residual disease determined this choice of adjuvant
treatment. A group of 12 patients with advanced disease (7 melanoma, 4 colorectal carcinoma, 1 kidney carcinoma) were treated
with TIL (median 5.8×1010 cells) and IL-2 (West’s schedule) modulated towards a lower dosage (from 12 to 6 MIU/day) in order to maintain an acceptable
level of toxicity. As treatment was well tolerated, it was offered to another 22 patients in an adjuvant setting after metastasectomy
(11 melanoma, 10 colorectal carcinoma, 1 renal cancer), the median dose of TIL reinfused being 4.95×1010 cells. No objective response was observed in advanced patients: all patients progressed after a median of 1.5 months (0–8
months) and median survival was 8 months (3–22+ months). Thirteen patients from the second group are still disease-free after
a median of 23+ months (9+–47+ months). The remaining 9 patients relapsed after a median of 5 months (3–18 months). Toxicity
was moderate as clinical and hepatic/renal function parameters were used to assess the need for dose reductions. Consequently,
there was great diversity in IL-2 dosages administered. In particular, there seemed to be a difference in IL-2 doses administered
between disease-free cases and those who progressed (17.5 MIU/day versus 7 MIU/day in melanoma patients; 11.2 MIU/day versus
7.1 MIU/day in colorectal cancer patients). By contrast, no differences were observed between number of TIL reinfused and
clinical response. Phenotypical characteristics of reinfused TIL were similar to those reported in the literature: 97% were
CD3 and 92% were CD8. Aspecific cytolytic activity was evaluated on 12 cases whereas, in 2 melanoma cases, autologous tumour
tissue was available for the specific cytotoxicity test. Perforin levels in TIL measured at the end of culture were generally
high or very high. Cytokine levels were measured on the supernatant at the end of culture, with an estreme variability in
results. Finally, ζ chain and p56lck were histologically assessed on the resected tissue from which TIL were cultivated. There were virtually none of the former
and a complete absence of the latter, which concurs with data reported in the literature. The same immunocytochemical analysis
was carried out on TIL at the end of culture. This time an almost complete restoration of both functions was seen, especially
in melanoma patients, who are still free from disease. The study is on-going and it has been decided to focus on disease-free
patients after metastasectomy in order to increase the number and possibility of clinical and histological correlations. 相似文献
128.