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排序方式: 共有221条查询结果,搜索用时 46 毫秒
71.
Poland DC Schalkwijk CG Stehouwer CD Koeleman CA van het Hof B van Dijk W 《Glycoconjugate journal》2001,18(3):261-268
Diabetic mellitus is attended by the development of endothelial dysfunction which is suggested to be accompanied with a chronic low-degree of inflammation. During a chronic hepatic inflammatory response, specific changes in glycosylation of the acute phase protein 1-acid glycoprotein (AGP) can be detected. In this report we studied the changes in glycosylation of AGP in more detail and evaluated the relation between a change in glycosylation of AGP and urinary albumin secretion in Type I diabetic patients. The glycosylation of AGP, studied by crossed affinity immunoelectrophoresis (CAIE) and high pH anion exchange chromatography with pulse amperometric detection (HPAEC-PAD), showed an increase in 3-fucosylation. Staining with an antibody against sialyl Lewisx (sLex) implied that part of the 3-fucosylation was present in a sLex-conformation. In the group of Type I diabetic patients with increased urinary albumin excretion, a significant increase in 3-fucosylation of AGP (p[emsp4 ]<[emsp4 ]0.0005) could be detected. Therefore, the increased 3-fucosylation of AGP can be used as an additional marker for the development of vascular complications in Type I diabetic patients. 相似文献
72.
Poland D 《Biopolymers》2001,58(5):477-490
We illustrate a new method for the determination of the complete binding polynomial for nucleic acids based on experimental titration data with respect to ligand concentration. From the binding polynomial, one can then calculate the distribution function for the number of ligands bound at any ligand concentration. The method is based on the use of a finite set of moments of the binding distribution function, which are obtained from the titration curve. Using the maximum-entropy method, the moments are then used to construct good approximations to the binding distribution function. Given the distribution functions at different ligand concentrations, one can calculate all of the coefficients in the binding polynomial no matter how many binding sites a molecule has. Knowledge of the complete binding polynomial in turn yields the thermodynamics of binding. This method gives all of the information that can be obtained from binding isotherms without the assumption of any specific molecular model for the nature of the binding. Examples are given for the binding of Mn(2+) and Mg(2+) to t-RNA and for the binding of Mg(2+) and I(6) to poly-C using literature data. 相似文献
73.
A P Poland E Glover J R Robinson D W Nebert 《The Journal of biological chemistry》1974,249(17):5599-5606
74.
Helen E. Roy Emma Rhule Susanne Harding Lori-Jayne Lawson Handley Remy L. Poland Eric W. Riddick Tove Steenberg 《BioControl》2011,56(4):663-679
Harmonia axyridis is an invasive alien predator in many countries across the world. The rapid establishment and spread of this species is of
concern because of the threat it poses to biodiversity as a generalist predator. Understanding the mechanisms that contribute
to the success of this species as an invader is not only intriguing but also critical to our understanding of the processes
governing such invasions. The enemy release hypothesis (ERH) could explain the rapid population growth of many invasive alien
species. However, empirical evidence in support of the ERH is lacking. An alternative hypothesis that could explain rapid
population growth is evolution of increased competitive ability (EICA). Here we provide an overview of the parasites and pathogens
of coccinellids with a particular focus on H. axyridis as a host. We examine the differential susceptibility of host species and highlight the resilience of H. axyridis in comparison to other coccinellids. We recognise the paucity and limitations of available information and suggest that studies,
within a life-table framework, comparing life history traits of H. axyridis in both the native and introduced ranges are necessary. We predict that H. axyridis could benefit from both enemy release and EICA within the introduced range but require further empirical evidence. 相似文献
75.
Helen E. Roy Patrick De Clercq Lori-Jayne Lawson Handley Remy L. Poland John J. Sloggett Eric Wajnberg 《BioControl》2011,56(4):375-382
Invasive alien species (IAS) coupled with climate change have been referred to as a “deadly duo”. Until recently research
on invasion biology has centred mainly on alien plants and vertebrates, despite the numerical dominance of alien arthropods.
Arthropods are the largest group of IAS worldwide and many can play a beneficial role, particularly in controlling insect
and mite pests. Indeed, 1590 terrestrial arthropod species have been identified as alien to Europe but only a fraction has
been shown to cause either an ecological or economical impact, yet knowledge is severely limited by a paucity of data. The
IOBC/WPRS Working Group “Benefits and Risks of Exotic Biological Control Agents” developed the theme of this special issue
to begin to address the limitations in understanding of this important research area. It represents a timely synthesis of
current ecological knowledge and research on alien arthropod predators and parasitoids. 相似文献
76.
Poland GA Ovsyannikova IG Kennedy RB Haralambieva IH Jacobson RM 《Omics : a journal of integrative biology》2011,15(9):625-636
In this article we define vaccinomics as the integration of immunogenetics and immunogenomics with systems biology and immune profiling. Vaccinomics is based on the use of cutting edge, high-dimensional (so called "omics") assays and novel bioinformatics approaches to the development of next-generation vaccines and the expansion of our capabilities in individualized medicine. Vaccinomics will allow us to move beyond the empiric "isolate, inactivate, and inject" approach characterizing past vaccine development efforts, and toward a more detailed molecular and systemic understanding of the carefully choreographed series of biological processes involved in developing viral vaccine-induced "immunity." This enhanced understanding will then be applied to overcome the obstacles to the creation of effective vaccines to protect against pathogens, particularly hypervariable viruses, with the greatest current impact on public health. Here we provide an overview of how vaccinomics will inform vaccine science, the development of new vaccines and/or clinically relevant biomarkers or surrogates of protection, vaccine response heterogeneity, and our understanding of immunosenescence. 相似文献
77.
Henriksen Hellyer JM DeVries AS Jenkins SM Lackore KA James KM Ziegenfuss JY Poland GA Tilburt JC 《PloS one》2011,6(12):e29478
Background
Though recommended by many and mandated by some, influenza vaccination rates among health care workers, even in pandemics, remain below optimal levels. The objective of this study was to assess vaccination uptake, attitudes, and distinguishing characteristics (including doctor-nurse differences) of health care workers who did and did not receive the pandemic H1N1 influenza vaccine in late 2009.Methodology/Principal Findings
In early 2010 we mailed a self-administered survey to 800 physicians and 800 nurses currently licensed and practicing in Minnesota. 1,073 individuals responded (cooperation rate: 69%). 85% and 62% of Minnesota physicians and nurses, respectively, reported being vaccinated. Accurately estimating the risk of vaccine side effects (OR 2.0; 95% CI 1.5–2.7), agreeing with a professional obligation to be vaccinated (OR 10.1; 95% CI 7.1–14.2), an ethical obligation to follow public health authorities'' recommendations (OR 9.9; 95% CI 6.6–14.9), and laws mandating pandemic vaccination (OR 3.1; 95% CI 2.3–4.1) were all independently associated with receiving the H1N1 influenza vaccine.Conclusions/Significance
While a majority of health care workers in one midwestern state reported receiving the pandemic H1N1 vaccine, physicians and nurses differed significantly in vaccination uptake. Several key attitudes and perceptions may influence health care workers'' decisions regarding vaccination. These data inform how states might optimally enlist health care workers'' support in achieving vaccination goals during a pandemic. 相似文献78.
Ovsyannikova IG Haralambieva IH Vierkant RA O'Byrne MM Jacobson RM Poland GA 《Human heredity》2011,72(3):206-223
79.
80.
Martin Mascher Gary J. Muehlbauer Daniel S. Rokhsar Jarrod Chapman Jeremy Schmutz Kerrie Barry María Muñoz‐Amatriaín Timothy J. Close Roger P. Wise Alan H. Schulman Axel Himmelbach Klaus F.X. Mayer Uwe Scholz Jesse A. Poland Nils Stein Robbie Waugh 《The Plant journal : for cell and molecular biology》2013,76(4):718-727
Next‐generation whole‐genome shotgun assemblies of complex genomes are highly useful, but fail to link nearby sequence contigs with each other or provide a linear order of contigs along individual chromosomes. Here, we introduce a strategy based on sequencing progeny of a segregating population that allows de novo production of a genetically anchored linear assembly of the gene space of an organism. We demonstrate the power of the approach by reconstructing the chromosomal organization of the gene space of barley, a large, complex and highly repetitive 5.1 Gb genome. We evaluate the robustness of the new assembly by comparison to a recently released physical and genetic framework of the barley genome, and to various genetically ordered sequence‐based genotypic datasets. The method is independent of the need for any prior sequence resources, and will enable rapid and cost‐efficient establishment of powerful genomic information for many species. 相似文献