Regulation of dendritic spine morphology by SPIN90, a novel Shank binding partner

Dendritic spines are highly specialized actin-rich structures on which the majority of excitatory synapses are formed in the mammalian CNS. SPIN90 is an actin-binding protein known to be highly enriched in postsynaptic densities (PSDs), though little is known about its function there. Here, we show that SPIN90 is a novel binding partner for Shank proteins in the PSD. SPIN90 and Shank co-immunoprecipitate from brain lysates and co-localize in postsynaptic dendrites and act synergistically to mediate spine maturation and spine head enlargement. At the same time, SPIN90 causes accumulation of Shank and PSD-95 within dendritic spines. In addition, we found that the protein composition of PSDs in SPIN90 knockout mice is altered as is the actin cytoskeleton of cultured hippocampal SPIN90 knockout neurons. Taken together, these findings demonstrate that SPIN90 is a Shank1b binding partner and a key contributor to the regulation of dendritic spine morphogenesis and brain function.     

Exploration of proteomic biomarkers and digital imaging analysis for Oryctes rhinoceros nudivirus infection

Oryctes rhinoceros nudivirus (OrNV) infects the larval stage of many coleopteran insects; however, the underlying mechanisms and biomarkers of infection are not fully characterised. In this study, an optimal culture condition was developed for OrNV replication and proteomic biomarkers were identified using comparative proteomic analysis. The highest level of viral copy number was observed in Sf9 cells treated with 450 μM of H₂O₂ and 2% foetal bovine serum (FBS). Among the 48 identified proteins, 14 proteins were significantly modulated in 2% FBS and H₂O₂- treated OrNV-infected cells (F2V) as compared with 10% FBS treated non-infected cells (F10M). Network analysis revealed that SLC25A5, VDAC3, PHB2, and ANXA1 act as signature proteins for OrNV replication. Moreover, viral envelope glycoproteins, GRBNV_gp28-like and GrBNV_gp62-like proteins could be used as sensitive diagnostic signatures for OrNV infection. Furthermore, to conveniently identify the OrNV-infection in Allomyrina dichotoma larvae, an image classification model was trained by Google Teachable Machine, which distinguished images with accuracy rates of 91% and 86% for infected and non-infected larvae, respectively, at a learning rate of 0.001. This study demonstrated that Sf9 cell medium treated with 2% FBS and 450 μM H₂O₂ is a permissible culture condition for OrNV replication. Proteomic signatures may be involved in the progression of viral infection. Additionally, a low-cost and non-invasive machine learning-derived digital imaging analysis may improve the prediction of OrNV infection in larvae.     

A Novel MXene/Ecoflex Nanocomposite-Coated Fabric as a Highly Negative and Stable Friction Layer for High-Output Triboelectric Nanogenerators

The triboelectric material properties and mechanical stability of the contact layer are vital to achieving durable triboelectric nanogenerators (TENGs) with high output performance. Herein, a novel MXene/Ecoflex nanocomposite is introduced as a promising triboelectric material because of its highly negative triboelectric properties and mechanical stability. The MXene/Ecoflex nanocomposite with a fabric-based waterproof TENG (FW-TENG) is fabricated and designed to universally harvest energy from various human motions as well as the natural environment (rain and wind). The fabricated FW-TENG delivers a maximum output peak power of 3.69 mW and a power density of 9.24 W m⁻², respectively, at a matching load resistance of 4.5 MΩ under a frequency of 4.5 Hz and a force of 8 N. Furthermore, the applicability of this device in various products is investigated. The FW-TENG can protect against a crash caused by rainy and humid weather. An FW-TENG-based self-powered smart active device that detects motion on a carpet is demonstrated and is equipped with sleep monitoring motion sensors. The FW-TENG not only has self-powered benefits and excellent mechanical amenability but is also exceptionally reliable and stable against water intrusion, which are important characteristics to realize next-generation wearable/portable technologies.     

Exploitation and community engagement: Can Community Advisory Boards successfully assume a role minimising exploitation in international research?

It has been suggested that community advisory boards (CABs) can play a role in minimising exploitation in international research. To get a better idea of what this requires and whether it might be achievable, the paper first describes core elements that we suggest must be in place for a CAB to reduce the potential for exploitation. The paper then examines a CAB established by the Shoklo Malaria Research Unit under conditions common in resource‐poor settings – namely, where individuals join with a very limited understanding of disease and medical research and where an existing organisational structure is not relied upon to serve as the CAB. Using the Tak Province Border Community Ethics Advisory Board (T‐CAB) as a case study, we assess the extent to which it might be able to take on a role minimising exploitation were it to decide to do so. We investigate whether, after two years in operation, T‐CAB is capable of assessing clinical trials for exploitative features and addressing those found to have them. The findings show that, although T‐CAB members have gained knowledge and developed capacities that are foundational for one‐day taking on a role to reduce exploitation, their ability to critically evaluate studies for the presence of exploitative elements has not yet been strongly demonstrated. In light of this example, we argue that CABs may not be able to perform such a role for a number of years after initial formation, making it an unsuitable responsibility for many short‐term CABs.     

Case studies of the history and politics of wild canid restoration in Korea

Wild canids were historically abundant in Korea; however, the gray wolf, dhole, and red fox were extirpated during the twentieth century. The causes varied. “Pest control” during the Japanese occupation, ecological destruction during wars, disease epizootics, and “vermin control” after the Korean War contributed to the complete demise of wolves. The fox had succumbed to unregulated hunting, rodenticides, habitat loss, and disease epizootics. The dhole was naturally rare; its extirpation from northeastern Asia including Korea is not established. Although the wolf and fox are extirpated, the Korean government still lists both as endangered species to facilitate the recently implemented restoration programs. Restoration will face the challenges of importing genetically diverse populations and the critical loss, fragmentation, and alteration of peri‐urban habitats. The overall social support for these efforts is not clear: it may be low because of changes in social mores or simply an unintended consequence of land and water use choices and policies that people may not perceive in everyday life. In this critical analysis, we postulate that the current restoration programs are misdirected toward inappropriate species and likely employ outdated techniques. We propose that a reallocation of restoration efforts and resources to populations of existing rare or threatened species would be more ecologically beneficial with higher probabilities of success. We recognize that there can be good reason to restore the upper trophic levels, especially keystone species, but are concerned that the impetus is more about focusing on charismatic megafauna rather than pragmatic choices more likely to be effective.