全文获取类型
收费全文 | 79篇 |
免费 | 7篇 |
专业分类
86篇 |
出版年
2023年 | 1篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 6篇 |
2014年 | 3篇 |
2013年 | 6篇 |
2012年 | 11篇 |
2011年 | 5篇 |
2010年 | 1篇 |
2009年 | 4篇 |
2008年 | 5篇 |
2006年 | 2篇 |
2005年 | 4篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 2篇 |
2001年 | 1篇 |
1999年 | 1篇 |
1996年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1980年 | 1篇 |
排序方式: 共有86条查询结果,搜索用时 0 毫秒
21.
Samuele A Mangiagalli A Armentero MT Fancellu R Bazzini E Vairetti M Ferrigno A Richelmi P Nappi G Blandini F 《Biochimica et biophysica acta》2005,1741(3):325-330
Wilson's disease (WD) is an inherited disorder, characterized by selective copper deposition in liver and brain, chronic hepatitis and extra-pyramidal signs. In this study, we investigated changes of biochemical markers of oxidative stress and apoptosis in liver, striatum and cerebral cortex homogenates from Long-Evans Cinnamon (LEC) rats, a mutant strain isolated from Long Evans (LE) rats, in whom spontaneous hepatitis develops shortly after birth. LEC and control (LE) rats at 11 and 14 weeks of age were used. We determined tissue levels of glutathione (GSH/GSSG ratio), lipid peroxides, protein-thiols (P-SH), nitric oxide metabolites, activities of caspase-3 and total superoxide-dismutase (SOD), striatal levels of monoamines and serum levels of hepatic amino-transferases. We observed a decrease of protein-thiols, GSH/GSSG ratio and nitrogen species associated to increased lipid peroxidation in the liver and striatum - but not in the cerebral cortex - of LEC rats, accompanied by dramatic increase in serum amino-transferases and decrease of striatal catecholamines. Conversely, SOD and caspase-3 activity increased consistently only in the cortex of LEC rats. Hence, we assume that enhanced oxidative stress may play a central role in the cell degeneration in WD, at the main sites of copper deposition, with discrete pro-apoptotic conditions developing in distal areas. 相似文献
22.
Plinio T. Cristofoletti Edson L. Kemper Adriana N. Capella Sandra R. Carmago Juliana L. Cazoto Fernanda Ferrari Tederson L. Galvan Luciane Gauer Gustavo A. Monge Marcelo A. Nishikawa Nicolas Z. Santos Altair A. Semeao Leandro Silva Alan R. Willse Almir Zanca Michael D. Edgerton 《Tropical plant biology》2018,11(1-2):17-30
Sugarcane borer (Diatraea saccharalis) is a major pest of sugarcane (Saccharum spp. hybrids), across the Americas. The insect is partially controlled by biological and chemical means, but still causes significant economic losses to sugarcane growers and processors. Proteins derived from Bacillus thuringiensis, Bt proteins, have been used to control sugarcane borer in maize (Zea mays) for the past decade. In sugarcane, the expression of individual Bt proteins has been reported several times. However practical use of Bt proteins requires their use as part of an Integrated Pest Management (IPM) system that includes the delivery of high doses of protein and the use of a refuge to slow the evolution of insect resistance to the protein. Here we demonstrate the feasibility of using Bt proteins to protect sugarcane from sugarcane borer in a commercial setting. We have expressed two Bt proteins with differing modes of action (Cry1Ab and Cry2Ab) in commercial sugarcane varieties, demonstrated efficacy against sugarcane borer in the field and describe a strategy for trait deployment in this tropical crop with complex genetics that limits trait introgression by backcrossing. 相似文献
23.
Milad?RadiomEmail authorView authors OrcID profile Plinio?Maroni Tomasz?A.?Wesolowski 《Journal of molecular modeling》2018,24(1):36
Using MP2, CCSD, and B3LYP methods of computational chemistry, we show length dependence in the intrinsic elastic properties of short alkane fragments. For isolated alkane fragments of finite length in the gas phase and zero temperature, the intrinsic elasticity constants are found to vary with the number of carbon atoms and its parity. From extrapolation of the elasticity constants calculations to infinite chain length, and by comparing with in-situ elasticity constant of single poly(ethylene) molecule obtained with atomic force microscopy, we estimate the softening effect of environment on the extension response of the polymer. 相似文献
24.
25.
Plinio C. Casarotto Mykhailo Girych Senem M. Fred Vera Kovaleva Rafael Moliner Giray Enkavi Caroline Biojone Cecilia Cannarozzo Madhusmita Pryiadrashini Sahu Katja Kaurinkoski Cecilia A. Brunello Anna Steinzeig Frederike Winkel Sudarshan Patil Stefan Vestring Tsvetan Serchov Cassiano R.A.F. Diniz Liina Laukkanen Eero Castrén 《Cell》2021,184(5):1299-1313.e19
26.
Andrea Ferrigno Cesarina Gregotti Plinio Richelmi Mariapia Vairetti 《In vitro cellular & developmental biology. Animal》2010,46(5):445-449
In the present study, the effects of dexamethasone on cadmium-induced toxicity were evaluated in isolated rat hepatocytes.
Hepatocytes were cultured for 24 h in William’s E medium containing fetal calf serum (10%), insulin (0.1 IU/ml), and glucagon
(0.01 μM) in the absence or presence of 0.1 μM dexamethasone. Cadmium chloride, 5 or 10 μM, was added to the medium and the
toxicity was evaluated for up to 48 h after treatment. Lactate dehydrogenase (LDH) release, the reduced and oxidized glutathione
ratio (GSH/GSSG), protein-SH groups, and lipid peroxidation levels were evaluated. Cadmium induced a dose- and time-dependent
LDH release in control hepatocytes at 24 h (Cd 10 μM 42%) while hepatocytes pretreated with dexamethasone showed lower necrosis
(Cd 10 μM 12% at 24 h). GSH/GSSH ratio and protein-SH groups were higher while lipid peroxidation was lower in dexamethasone-treated
hepatocytes as compared with untreated cells. In conclusion, cadmium toxicity was associated with an increase in intracellular
oxidative stress responsible for accelerated cell death. The use of dexamethasone prevented cadmium damage, suggesting that
the cytoprotective action of this hormone is related to its effect in preventing changes in thiols such as glutathione and
protein-SH groups. 相似文献
27.
Souza Teixeira C da Silva HC de Moura TR Pereira-Júnior FN do Nascimento KS Nagano CS Sampaio AH Delatorre P Rocha BA Cavada BS 《Journal of biochemistry》2012,152(1):87-98
Lectins have been used as models for studies of the molecular basis of protein-carbohydrate interaction and specificity by deciphering codes present in the glycan structures. The purpose of the present study was to purify and solve the complete primary and crystal structure of the lectin of Camptosema pedicellatum (CPL) complexed with 5-bromo-4-chloro-3-indolyl-α-d-mannose (X-Man) using tandem mass spectrometry. CPL was purified by single-step affinity chromatography. Mass spectrometry findings revealed that purified CPL features a combination of chains weighing 25,298 ± 2 (α-chain), 12,835 ± 2 (β-chain) and 12,481 ± 2 Da (γ-chain). The solved crystal structure of CPL features a conservative mutation in the hydrophobic subsite, a constituent of the carbohydrate recognition domain (CRD), indicating the relevance of hydrophobic interactions in the establishment of interactions with carbohydrates. The substitution and the analysis of the interactions with X-Man also revealed that the hydrophobic effect caused by a minor change in the hydrophobic subsite interferes in the formation of H-bonds due to the reorientation of the indolyl group in the CRD. 相似文献
28.
Ribonuclease III (RNase III) type of enzymes are double-stranded RNA (dsRNA)-specific endoribonucleases that have important roles in RNA maturation and mRNA decay. They are involved in processing precursors of ribosomal RNA (rRNA) in bacteria as well as precursors of short interfering RNAs (siRNAs) and microRNAs (miRNAs) in eukaryotes. RNase III proteins have been grouped in three major classes according to their domain organization. In this issue of Molecular Microbiology, Redko et al. identified a novel class of bacterial RNase III, named Mini-III, consisting only of the RNase III catalytic domain and functioning in the maturation of the 23S rRNA in Bacillus subtilis. Its absence from proteobacteria reveals that this step is mechanistically different from the corresponding step in Escherichia coli. The fact that Mini-III orthologues are present in unicellular photosynthetic eukaryotes and in plants opens new opportunities for functional studies of this type of RNases. 相似文献
29.
Plinio Delatorre José Caetano Silva-Filho Bruno Anderson Matias Rocha Tatiane Santi-Gadelha Raphael Batista da Nóbrega Carlos Alberto Almeida Gadelha Kyria Santiago do Nascimento Celso Shiniti Nagano Alexandre Holanda Sampaio Benildo Sousa Cavada 《Biochimie》2013
Indole-3-acetic acid (IAA) bound is considered a storage molecule and is inactive. However, some studies have proposed an additional possible regulatory mechanism based on the ability of lectins to form complexes with IAA. We report the first crystal structure of ConM in complex with IAA at 2.15 Å resolution. Based on a tetrameric model of the complex, we hypothesize how the lectin controls the availability of IAA during the early seedling stages, indicating a possible new physiological role for these proteins. A free indole group is also bound to the protein. The ConM interaction with different forms of IAA is a strategy to render the phytohormone unavailable to the cell. Thus, this new physiological role proposed for legume lectins might be a novel mechanism by which IAA levels are decreased in addition to the destruction and formation of new complexes in the later stages of seed germination. 相似文献
30.
Survival, duration of intermoult cycle and respiratory metabolism were evaluated as a function of salinity (0–35‰; 25° C)
in early zoeae of the cinnamon shrimp, Macrobrachium amazonicum. Zoeae are extremely resistant to salinity, mortality occurring only in fresh and sea-water after several days. Moulting
occurs in all salinities, longer cycles being recorded in 0 and 35‰ S. The metabolism-salinity curve is broadly U-shaped between
0 and 28‰ S but declines sharply in sea-water. Such physiological responses characterise the early zoeae as strongly euryhaline
and typically estuarine. Data are discussed in relation to the degree of adaptation of the organism to the freshwater biotope
and the position of the species within the generic pattern of adaptive radiation. 相似文献