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41.
Putz FE Zuidema PA Pinard MA Boot RG Sayer JA Sheil D Sist P;Elias Vanclay JK 《PLoS biology》2008,6(7):e166
42.
Ribonuclease III (RNase III) type of enzymes are double-stranded RNA (dsRNA)-specific endoribonucleases that have important roles in RNA maturation and mRNA decay. They are involved in processing precursors of ribosomal RNA (rRNA) in bacteria as well as precursors of short interfering RNAs (siRNAs) and microRNAs (miRNAs) in eukaryotes. RNase III proteins have been grouped in three major classes according to their domain organization. In this issue of Molecular Microbiology, Redko et al. identified a novel class of bacterial RNase III, named Mini-III, consisting only of the RNase III catalytic domain and functioning in the maturation of the 23S rRNA in Bacillus subtilis. Its absence from proteobacteria reveals that this step is mechanistically different from the corresponding step in Escherichia coli. The fact that Mini-III orthologues are present in unicellular photosynthetic eukaryotes and in plants opens new opportunities for functional studies of this type of RNases. 相似文献
43.
Midgut adaptation and digestive enzyme distribution in a phloem feeding insect,the pea aphid Acyrthosiphon pisum 总被引:1,自引:0,他引:1
Cristofoletti PT Ribeiro AF Deraison C Rahbé Y Terra WR 《Journal of insect physiology》2003,49(1):11-24
Transmission electron micrographs of the pea aphid midgut revealed that its anterior region has cells with an apical complex network of lamellae (apical lamellae) instead of the usual regularly-arranged microvilli. These apical lamellae are linked to one another by trabeculae. Modified perimicrovillar membranes (MPM) are associated with the lamellae and project into the lumen. Trabeculae and MPM become less conspicuous along the midgut. The most active A. pisum digestive enzymes are membrane-bound. An aminopeptidase (APN) is described elsewhere. An alpha-glucosidase (alpha-Glu) has a molecular mass of 72 kDa, pH optimum 6.0 and catalyzes in vitro transglycosylations in the presence of an excess of the substrate sucrose. There is a major cysteine proteinase activity (CP) on protein substrates that has a molecular mass of 40 kDa, pH optimum 5.5, is inhibited by E-64 and chymostatin and is activated by EDTA+cysteine. The enzyme is more active against carbobenzoxy-Phe-Arg-4-methylcoumarin-7-amide (ZFRMCA) than against ZRRMCA. These features identify the purified CP as a cathepsin-L-like cysteine proteinase. Most CP is found in the anterior midgut, whereas alpha-Glu and APN predominate in the posterior midgut. With the aid of antibodies, alpha-Glu and CP were immunolocalized in cell vesicles and MPM, whereas APN was localized in vesicles, apical lamellae and MPM. The data suggest that the anterior midgut is structurally reinforced to resist osmotic pressures and that the transglycosylating alpha-Glu, together with CP and APN are bound to MPM, thus being both distributed over a large surface and prevented from excretion with honeydew. alpha-Glu frees glucose from sucrose without increasing the osmolarity, and CP and APN may process toxins or other proteins occasionally present in phloem. 相似文献
44.
45.
Andrea Ferrigno Filippo Carlucci Antonella Tabucchi Valentina Tommassini Vittoria Rizzo Plinio Richelmi Enrico Gringeri Daniele Neri Eleonora Boncompagni Isabel Freitas Umberto Cillo Mariapia Vairetti 《Cryobiology》2009,59(3):327-334
We compared the susceptibility of liver grafts from lean and obese Zucker rats to preservation injury, using two organ-preservation techniques: conventional static preservation (SP) and machine perfusion (MP) preservation. SP: livers preserved by UW solution at 4, 8 or 20 °C for 6-h. MP: livers perfused for 6-h with an improved oxygenated Krebs–Henseleit solution (KH) at 4, 8 or 20 °C. Reperfusion with KH (2-h) was performed either with the SP or MP preserved livers. Fatty livers tolerate SP poorly at 4, 8 and 20 °C as compared with MP at the same temperatures. SP induced a decrease in the ATP/ADP ratio both at 8 and 20 °C in obese rats while an increase in energy status was found with MP at 8 and 20 °C. Nitrate/nitrite (NOx) concentration was higher and bile flow lower in livers preserved with SP than MP. In lean rats, no differences were observed between MP and SP as regards enzyme release, bile production and NOx levels except for SP at 20 °C in which high enzyme release and low bile flow were observed. In lean rats ATP/ADP was higher and NOx was lower with MP at 20 °C than with SP at 20 °C. To optimize steatotic liver preservation SP should be avoided because it is particularly detrimental as compared with MP. 相似文献
46.
The ATP-dependent sequestration of Ca2+ by the plasma membrane fraction from rat liver is stimulated by reduced glutathione and dithiothreitol and inhibited by diamide and t-butyl hydroperoxide. The inhibitory effect on Ca2+ sequestration by the oxidizing agents is prevented in the presence of the thiols. Our results therefore suggest that free sulfhydryl group(s) may be critical for the activity of hepatic plasma membrane Ca2+ translocase, and that inhibition of this activity by the oxidation of such group(s) may contribute to the perturbation of Ca2+ homeostasis during oxidative stress. 相似文献
47.
Telomeres are specialized caps of nucleoprotein complexes located at the chromosome termini. They consist of short DNA repeats and of an assortment of associated proteins whose function is currently under intense investigation in model systems. These specialized structures protect the linear ends of eukaryotic chromosomes against DNA repair and degradation activities, and serve as the substrate for telomerase, the ribonucleoprotein complex that synthesises the telomere repeats. The pivotal role of the telomeres in the maintenance of cell viability in several model eukaryotes, including humans, greatly promoted research in telomere biology. Studies on telomere structure and function in fungi other than model systems are limited to providing information on the telomeric repeat sequences. Here, we have summarized the current knowledge on the organization of chromosome ends and on the proteins participating in telomere function in model systems including recent information obtained for filamentous fungi. We also describe Ustilago maydis genes that are potential homologs of proteins known from other systems to participate in telomere biology. 相似文献
48.
Francisco Javier Ruiz-Sánchez Matthijs Freudenthal Samuel Mansino Vicente Daniel Crespo Plinio Montoya 《Pal?ontologische Zeitschrift》2014,88(1):85-98
Apocricetus Freudenthal et al. (Treballs del Museu de Geologia de Barcelona 7: 11–93, 1998) is a medium to large Cricetinae, practically without mesolophids in the m1 and m2, with long third molars; anterior and posterior protolophule are present and the posterior metalophule is reduced or absent. Apocricetus barrierei (Mein and Michaux Comptes Rendus Academie de Sciences de Paris D 270: 2780–2783, 1970) was defined on the basis of three specimens from the French locality Chabrier. Apart from this site, several French and Spanish localities of Early Pliocene age contain remains of this species. Despite this extra material, the species is poorly characterized, and none of the localities contains material of all six dental elements. In this paper, two new assemblages from La Bullana 2B and La Bullana 3 (Cabriel Basin, Spain) are described. The rich assemblage from La Bullana 2B provides a better insight in the metrical and morphological variability of this species. 相似文献
49.
The Tropical managed Forests Observatory: a research network addressing the future of tropical logged forests 下载免费PDF全文
Plinio Sist Ervan Rutishauser Marielos Peña-Claros Alexander Shenkin Bruno Hérault Lilian Blanc Christopher Baraloto Fidèle Baya Fabrice Benedet Katia Emidio da Silva Laurent Descroix Joice Nunes Ferreira Sylvie Gourlet-Fleury Marcelino Carneiro Guedes Ismail Bin Harun Riina Jalonen Milton Kanashiro Haruni Krisnawati Mrigesh Kshatriya Philippa Lincoln Lucas Mazzei Vincent Medjibé Robert Nasi Marcus Vinicius N. d'Oliveira Luis C. de Oliveira Nicolas Picard Stephan Pietsch Michelle Pinard Hari Priyadi Francis. E. Putz Ken Rodney Vivien Rossi Anand Roopsind Ademir Roberto Ruschel Nur Hajar Zamah Shari Cintia Rodrigues de Souza Farida Herry Susanty Eleneide Doff Sotta Marisol Toledo Edson Vidal Thales A.P. West Verginia Wortel Toshihiro Yamada 《应用植被学》2015,18(1):171-174
While attention on logging in the tropics has been increasing, studies on the long-term effects of silviculture on forest dynamics and ecology remain scare and spatially limited. Indeed, most of our knowledge on tropical forests arises from studies carried out in undisturbed tropical forests. This bias is problematic given that logged and disturbed tropical forests are now covering a larger area than the so-called primary forests. A new network of permanent sample plots in logged forests, the Tropical managed Forests Observatory (TmFO), aims to fill this gap by providing unprecedented opportunities to examine long-term data on the resilience of logged tropical forests at regional and global scales. TmFO currently includes 24 experimental sites distributed across three tropical regions, with a total of 490 permanent plots and 921 ha of forest inventories. 相似文献