Allometric scaling in the postcanine dentition with reference to primate diets

Allometric analysis of maxillary postcanine tooth area (Y) compared to adult skull length (X) in 34 species of higher primates has revealed a very close relationship. The derived least squares equation is logY=2.223 logX−2.311 with a correlation coefficient of .98. The value of 2.223 for the allometric coefficient suggests that tooth area may be in part related to the amount of food ingested. Although most of the species examined fit this equation very closely, several could be identified as being slightly deviant. In general, the direction of this deviance can be related to known dietary preferences. Relatively large molars are found in leaf-eaters (Alouatta, Presbytis, Colobus) and in the grass and seed eatingTheropithecus. Relatively small molars are found in primates with insectivorous preferences (Cebuella, Callithrix, Saguinus, andSaimiri) and in one frugivore (Ateles). The suggestion is made that the amount of mastication required by the preferred food is one factor in determining the most adaptive tooth size.     

Anabolic and catabolic responses of human articular chondrocytes to varying oxygen percentages

Introduction  

Oxygen is a critical parameter proposed to modulate the functions of chondrocytes ex-vivo as well as in damaged joints. This article investigates the effect of low (more physiological) oxygen percentage on the biosynthetic and catabolic activity of human articular chondrocytes (HAC) at different phases of in vitro culture.     

Fact and assumption in studies on the origins of life

Proteins and nucleic acids organise metabolic systems very efficiently. Original forms of life may have depended on less efficient mechanisms which have been superseded in the course of evolutionary selection. Discussion and experiment on the origins of life should therefore not depend on the assumption that substances and mechanisms, now seemingly essential, were essential initially. Instead we should consider what might happen in a few million years on moist mineral surfaces, smeared with bituminous material, and exposed to UV light in a reducing atmosphere in the absence of predators. What little evidence can be gathered from differences in compositon and metabolic behaviour between ancient and recently evolved species suggests that the trend in biochemical evolution is towards simplification.     

Analysis of leaf appearance,leaf death and phoma leaf spot,caused by Leptosphaeria maculans,on oilseed rape (Brassica napus) cultivars

Development of phoma leaf spot (caused by Leptosphaeria maculans) on winter oilseed rape (canola, Brassica napus) was assessed in two experiments at Rothamsted in successive years (2003–04 and 2004–05 growing seasons). Both experiments compared oilseed rape cultivars Eurol, Darmor, Canberra and Lipton, which differ in their resistance to L. maculans. Data were analysed to describe disease development in terms of increasing numbers of leaves affected over thermal time from sowing. The cultivars showed similar patterns of leaf spot development in the 2003–04 experiment when inoculum concentration was relatively low (up to 133 ascospores m⁻³ air), Darmor developing 5.3 diseased leaves per plant by 5 May 2004, Canberra 6.6, Eurol 6.8 and Lipton 7.5. Inoculum concentration was up to sevenfold greater in 2004–05, with Eurol and Darmor developing 2.4 diseased leaves per plant by 16 February 2005, whereas Lipton and Canberra developed 2.8 and 3.0 diseased leaves, respectively. Based on three defined periods of crop development, a piece-wise linear statistical model was applied to the progress of the leaf spot disease (cumulative diseased leaves) in relation to appearance (‘birth’) and death of leaves for individual plants of each cultivar. Estimates of the thermal time from sowing until appearance of the first leaf or death of the first leaf, the rate of increase in number of diseased leaves and the area under the disease progress line (AUDPL) for the first time period were made. In 2004–05, Canberra (1025 leaves ×°C days) and Lipton (879) had greater AUDPL values than Eurol (427) and Darmor (598). For Darmor and Lipton, the severity of leaf spotting could be related to the severity of stem canker at harvest. Eurol had less leaf spotting but severe stem canker, whereas Canberra had more leaf spotting but less severe canker.     

Alkali treatment of straw for ruminants. II. Nutritive value of straw ensiled after alkali treatment

In the first of two experiments barley straw was treated with a 16% solution of $\text{NaOH}\text{t}$ dry matter (DM) and ensiled for one year. It was satisfactorily preserved, having a pH of 10 and counts of 10⁴ mesophilic bacteria and 10³ fungal propagules per g. When this straw was mixed with concentrates (50:50, DM basis) and given to sheep, DM intake and digestibility were 102 g/kg W^0.75 per day and 67.3%. Comparable values for a diet based on freshly-treated straw were 88 g and 66.5%.In the second experiment, a 6-tonne batch of alkali-treated barley straw was ensiled for 2–4 months, then mixed with 40% concentrates and given to 370-kg steers for 66 days. Intake and growth rate were 10.23 and 1.08 kg/d, compared with 9.06 and 1.00 kg/d for steers given a diet based on freshly-treated straw. The latter diet was less digestible (for sheep) than the former.It is concluded that the feeding value of stored straw is as high as that of freshly-treated material.     

Aberrant protein N-glycosylation impacts upon infection-related growth transitions of the haploid plant-pathogenic fungus Mycosphaerella graminicola

The ascomycete fungus Mycosphaerella graminicola is the causal agent of Septoria Tritici Blotch disease of wheat and can grow as yeast-like cells or as hyphae depending on environmental conditions. Hyphal growth is however essential for successful leaf infection. A T-DNA mutagenesis screen performed on haploid spores identified a mutant, which can undergo yeast-like growth but cannot switch to hyphal growth. For this reason the mutant was non-pathogenic towards wheat leaves. The gene affected, MgAlg2, encoded a homologue of Saccharomyces cerevisiae ScAlg2, an alpha-1,2-mannosyltransferase, which functions in the early stages of asparagine-linked protein (N-) glycosylation. Targeted gene deletion and complementation experiments confirmed that loss of MgAlg2 function prevented the developmental growth switch. MgAlg2 was able to functionally complement the S. cerevisiae ScAlg2-1 temperature sensitive growth phenotype. Spores of ΔMgAlg2 mutants were hypersensitive to the cell wall disrupting agent Calcofluor white and produced abnormally hypo-N-glycosylated proteins. Gene expression, proteome and glycoproteome analysis revealed that ΔMgAlg2 mutant spores show responses typically associated with the accumulation of mis-folded proteins. The data presented highlight key roles for protein N-glycosylation in regulating the switch to hyphal growth, possibly as a consequence of maintaining correct folding and localization of key proteins involved in this process.     

Early evolutionary history of the flowering plant family Annonaceae: steady diversification and boreotropical geodispersal

Aim Rain forest‐restricted plant families show disjunct distributions between the three major tropical regions: South America, Africa and Asia. Explaining these disjunctions has become an important challenge in biogeography. The pantropical plant family Annonaceae is used to test hypotheses that might explain diversification and distribution patterns in tropical biota: the museum hypothesis (low extinction leading to steady accumulation of species); and dispersal between Africa and Asia via Indian rafting versus boreotropical geodispersal. Location Tropics and boreotropics. Methods Molecular age estimates were calculated using a Bayesian approach based on 83% generic sampling representing all major lineages within the family, seven chloroplast markers and two fossil calibrations. An analysis of diversification was carried out, which included lineage‐through‐time (LTT) plots and the calculation of diversification rates for genera and major clades. Ancestral areas were reconstructed using a maximum likelihood approach that implements the dispersal–extinction–cladogenesis model. Results The LTT plots indicated a constant overall rate of diversification with low extinction rates for the family during the first 80 Ma of its existence. The highest diversification rates were inferred for several young genera such as Desmopsis, Uvariopsis and Unonopsis. A boreotropical migration route was supported over Indian rafting as the best fitting hypothesis to explain present‐day distribution patterns within the family. Main conclusions Early diversification within Annonaceae fits the hypothesis of a museum model of tropical diversification, with an overall steady increase in lineages possibly due to low extinction rates. The present‐day distribution of species within the two largest clades of Annonaceae is the result of two contrasting biogeographic histories. The ‘long‐branch clade’ has been diversifying since the beginning of the Cenozoic and underwent numerous geodispersals via the boreotropics and several more recent long‐distance dispersal events. In contrast, the ‘short‐branch clade’ dispersed once into Asia via the boreotropics during the Early Miocene and further dispersal was limited.