全文获取类型
收费全文 | 922篇 |
免费 | 64篇 |
出版年
2023年 | 7篇 |
2022年 | 25篇 |
2021年 | 36篇 |
2020年 | 19篇 |
2019年 | 31篇 |
2018年 | 27篇 |
2017年 | 30篇 |
2016年 | 39篇 |
2015年 | 63篇 |
2014年 | 67篇 |
2013年 | 63篇 |
2012年 | 68篇 |
2011年 | 74篇 |
2010年 | 37篇 |
2009年 | 47篇 |
2008年 | 37篇 |
2007年 | 36篇 |
2006年 | 46篇 |
2005年 | 47篇 |
2004年 | 24篇 |
2003年 | 29篇 |
2002年 | 23篇 |
2001年 | 11篇 |
2000年 | 19篇 |
1999年 | 21篇 |
1998年 | 6篇 |
1997年 | 10篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 2篇 |
1993年 | 6篇 |
1992年 | 7篇 |
1990年 | 2篇 |
1988年 | 1篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1966年 | 1篇 |
1956年 | 1篇 |
1928年 | 1篇 |
排序方式: 共有986条查询结果,搜索用时 31 毫秒
981.
The Polycomb group protein MEDEA controls cell proliferation and embryonic patterning in Arabidopsis
Sara Simonini Marian Bemer Stefano Bencivenga Valeria Gagliardini Nuno D. Pires Bénédicte Desvoyes Eric van der Graaff Crisanto Gutierrez Ueli Grossniklaus 《Developmental cell》2021,56(13):1945-1960.e7
- Download : Download high-res image (284KB)
- Download : Download full-size image
982.
Mitochondrial DNA analysis of Rhipicephalus sanguineus s.l. from the western Iberian peninsula
下载免费PDF全文
![点击此处可从《Medical and veterinary entomology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
C. ALMEIDA R. SIMÕES M. J. COIMBRA‐DORES F. ROSA D. DIAS 《Medical and veterinary entomology》2017,31(2):167-177
Rhipicephalus sanguineus Latreille (1806) (Ixodida: Ixodidae) is considered to be the most widely distributed tick and to have a vast range of habitats and hosts, including livestock, pets and wildlife. In addition to morphological differences, recent investigations using approaches based on molecular genetic markers have revealed the existence of different R. sanguineus lineages in different geographic regions. In this study, 475 ticks collected from dogs in the western Iberian peninsula were studied both morphologically and genetically, using 12S and 16S rDNA and COI gene markers in order to clarify the controversy over the systematic status of R. sanguineus sensu lato in Western Europe, and to compare the present data with those sourced from studies conducted in other regions of the world. Despite the high morphometric variability, particularly on spiracles in both genders and in female genitalia, data obtained with different genetic molecular markers show very low variability, suggesting the existence of a unique species. In addition, the phylogenetic analysis showed genetic uniformity, supporting the existence of a well‐defined clade consisting of R. sanguineus s.l. specimens from Western Europe that are distinct from R. sanguineus s.l. from Africa. Furthermore, these data corroborate the existence of a polymorphic species in Western Europe, which requires to be consensually redescribed in view of its medical and veterinary importance in pathogen transmission. 相似文献
983.
Rinaldo Pires dos Santos Jorge Ernesto Araujo Mariath 《Biotechnic & histochemistry》1997,72(6):315-319
A simple method to cultivate pollen tubes in a gelatin medium is presented. After the growth of the pollen tubes in the culture medium, they are fixed, dehydrated, and embedded in resin for ultramicrotomy. The method is easy and does not require the purchase of special materials beyond those needed for the usual techniques for studying biological specimens under transmission electron microscopy. 相似文献
984.
985.
Claudine M. Carvalho Anésia A. Santos Silvana R. Pires Carolina S. Rocha Daniela I. Saraiva Jo?o Paulo B. Machado Eliciane C. Mattos Luciano G. Fietto Elizabeth P. B. Fontes 《PLoS pathogens》2008,4(12)
The NSP-interacting kinase (NIK) receptor-mediated defense pathway has been identified recently as a virulence target of the geminivirus nuclear shuttle protein (NSP). However, the NIK1–NSP interaction does not fit into the elicitor–receptor model of resistance, and hence the molecular mechanism that links this antiviral response to receptor activation remains obscure. Here, we identified a ribosomal protein, rpL10A, as a specific partner and substrate of NIK1 that functions as an immediate downstream effector of NIK1-mediated response. Phosphorylation of cytosolic rpL10A by NIK1 redirects the protein to the nucleus where it may act to modulate viral infection. While ectopic expression of normal NIK1 or a hyperactive NIK1 mutant promotes the accumulation of phosphorylated rpL10A within the nuclei, an inactive NIK1 mutant fails to redirect the protein to the nuclei of co-transfected cells. Likewise, a mutant rpL10A defective for NIK1 phosphorylation is not redirected to the nucleus. Furthermore, loss of rpL10A function enhances susceptibility to geminivirus infection, resembling the phenotype of nik1 null alleles. We also provide evidence that geminivirus infection directly interferes with NIK1-mediated nuclear relocalization of rpL10A as a counterdefensive measure. However, the NIK1-mediated defense signaling neither activates RNA silencing nor promotes a hypersensitive response but inhibits plant growth and development. Although the virulence function of the particular geminivirus NSP studied here overcomes this layer of defense in Arabidopsis, the NIK1-mediated signaling response may be involved in restricting the host range of other viruses. 相似文献
986.
1. Predictions from the Phylogenetic Constraints Hypothesis were tested for the first time in a tropical system using the pasture pest Deois flavopicta Stal, which oviposits into the ground and not into plant tissues. The prediction that there is no oviposition preference–larval performance linkage was supported. The absence of such a link provides an evolutionary basis for eruptive population dynamics. 2. The effects of host species and host plant quality on the preference of ovipositing females of D. flavopicta and performance of their offspring on the selected host plants were tested at the population level. 3. Female oviposition behaviour was affected by the presence of the host plant. Females of D. flavopicta showed a strong preference to oviposit close to host plants. The number of eggs was higher in pots containing Brachiaria ruziziensis (121.88 ± 13.70) than in pots containing only the wet oviposition substrate (5.2 ± 1.98) or dry oviposition substrate (0.067 ± 0.067). Ovipositing females did not, however, discriminate between plants of Brachiaria decumbens and Axonopus marginatus and did not show a strong oviposition preference in relation to differences in plant quality (protein and fibre content). They did show oviposition preference for plants under the high watering regime. The mean number of eggs collected from pots with non water‐stressed plants was 60% higher than the mean number of eggs collected on pots with water‐stressed plants. 4. Although females did not show ovipositional preference, spittlebug larval performance, measured as percentage survival and duration of nymphal period, was better on plants of high protein and low fibre content. These results indicate that there is not a linkage between female oviposition preference and subsequent nymphal performance in relation to differences in protein and fibre content in the host plants. There was, however, a limited linkage between oviposition preference and nymphal performance in relation to plant water content. Females showed preference for moist sites that have high survival of newly hatched nymphs. 5. Evidence indicates that for D. flavopicta, the influences of natality and female oviposition behaviour in response to plant quality are not the major factors driving population outbreaks, which is in accordance with the Phylogenetic Constraints Hypothesis. 相似文献