全文获取类型
收费全文 | 2518篇 |
免费 | 141篇 |
专业分类
2659篇 |
出版年
2023年 | 20篇 |
2022年 | 33篇 |
2021年 | 53篇 |
2020年 | 34篇 |
2019年 | 54篇 |
2018年 | 88篇 |
2017年 | 88篇 |
2016年 | 120篇 |
2015年 | 145篇 |
2014年 | 133篇 |
2013年 | 199篇 |
2012年 | 206篇 |
2011年 | 209篇 |
2010年 | 137篇 |
2009年 | 98篇 |
2008年 | 147篇 |
2007年 | 140篇 |
2006年 | 153篇 |
2005年 | 123篇 |
2004年 | 101篇 |
2003年 | 89篇 |
2002年 | 89篇 |
2001年 | 10篇 |
2000年 | 5篇 |
1999年 | 11篇 |
1998年 | 15篇 |
1997年 | 14篇 |
1996年 | 11篇 |
1995年 | 8篇 |
1994年 | 10篇 |
1993年 | 12篇 |
1992年 | 6篇 |
1991年 | 7篇 |
1990年 | 10篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1985年 | 4篇 |
1984年 | 7篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1981年 | 10篇 |
1980年 | 7篇 |
1979年 | 9篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1969年 | 2篇 |
排序方式: 共有2659条查询结果,搜索用时 0 毫秒
991.
992.
993.
994.
995.
996.
997.
998.
The morphological differentiation and taxonomic treatment of lowland and high-mountain morphotypes within the Solidago virgaurea group are controversial. To clarify the taxonomic status of these taxa, we conducted a morphometric analysis of 1,746 individuals from 80 localities along an altitudinal gradient from the lowlands of northern Poland to the Carpathians and Sudetes of southern Poland. Multivariate morphometric analyses, cluster analyses and principal component analyses, were used to examine the morphological differentiation within the S. virgaurea group in Poland. Canonical discriminant analysis was applied to determine the morphological characters that best discriminate among the taxa. The stability of the high-mountain Solidago minuta morphotype was tested in an experimental field established in lowland Poland; individuals transplanted from various mountain sites were cultivated at this site, and the morphotypes remained stable in terms of their floral and vegetative characters. Multivariate analyses revealed two morphologically distinct taxa in the S. virgaurea group, which correspond to lowland S. virgaurea s. str. and high-mountain S. minuta as recognised in some European floras. The most important morphological characters for distinguishing the taxa are the number of tubular florets per capitulum, inner involucral bract width and involucre height. Vegetative and inflorescence characters appear to have less taxonomic value because they changed continuously with altitude. A key for identifying S. virgaurea and S. minuta in Poland is presented. 相似文献
999.
Anna Jakimska Magdalena ?liwka-Kaszyńska Piotr Nagórski Jacek Namie?nik Agata Kot-Wasik 《PloS one》2014,9(10)
Nowadays, monitoring focuses on the primary compounds and does not include degradation products formed during various biological and chemical processes. Transformation products may have the same effects to human health and the environment or sometimes they can be more toxic than the parent compound. Unfortunately, knowledge about the formation of degradation products is still limited, however, can be very important for the environmental risk assessment. Firstly, the photodegradation kinetic of amlodipine was investigated in two experimental conditions: during the exposure to solar radiation and during the exposure to the light emitted by the xenon lamp. In all cases degradation of amlodipine followed a pseudo-first-order kinetics. In the next step, identification of transformation products of amlodipine formed during the exposure to xenon lamp irradiation was performed using ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS). As a result sixteen photoproducts were identified, their structures were elucidated and ultimately the transformation pathway was proposed. Fifteen compounds (out of 16 photoproducts) were newly identified and reported here for the first time; some of those compounds were formed from the first photoproduct, amlodipine pyridine derivative. Several analytes were formed only in acidic or basic conditions. Furthermore, the occurrence of amlodipine and its identified degradation products was investigated in environmental waters. Only one out of 16 compounds was found in wastewater effluent. The possibility of the sorption of examined analytes to sewage sludge particles was discussed based on QSAR. 相似文献
1000.
Agnieszka Szamborska-Gbur Grzegorz Rymarczyk Marek Or?owski Tomasz Kuzynowski Micha? Jakób Agnieszka Dziedzic-Letka Andrzej Górecki Piotr Dobryszycki Andrzej O?yhar 《PloS one》2014,9(1)
The heterodimer of the ecdysone receptor (EcR) and ultraspiracle (Usp), members of the nuclear receptors superfamily, regulates gene expression associated with molting and metamorphosis in insects. The DNA binding domains (DBDs) of the Usp and EcR play an important role in their DNA-dependent heterodimerization. Analysis of the crystal structure of the UspDBD/EcRDBD heterocomplex from Drosophila melanogaster on the hsp27 gene response element, suggested an appreciable similarity between both DBDs. However, the chemical denaturation experiments showed a categorically lower stability for the EcRDBD in contrast to the UspDBD. The aim of our study was an elucidation of the molecular basis of this intriguing instability. Toward this end, we mapped the EcRDBD amino acid sequence positions which have an impact on the stability of the EcRDBD. The computational protein design and in vitro analyses of the EcRDBD mutants indicate that non-conserved residues within the α-helix 2, forming the EcRDBD hydrophobic core, represent a specific structural element that contributes to instability. In particular, the L58 appears to be a key residue which differentiates the hydrophobic cores of UspDBD and EcRDBD and is the main reason for the low stability of the EcRDBD. Our results might serve as a benchmark for further studies of the intricate nature of the EcR molecule. 相似文献