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991.
Effect of Lower Versus Higher Protein Content in Infant Formula Through the First Year on Body Composition from 1 to 6 Years: Follow‐Up of a Randomized Clinical Trial 下载免费PDF全文
Martina Totzauer Veronica Luque Joaquin Escribano Ricardo Closa‐Monasterolo Elvira Verduci Alice ReDionigi Joana Hoyos Jean‐Paul Langhendries Dariusz Gruszfeld Piotr Socha Berthold Koletzko Veit Grote for The European Childhood Obesity Trial Study Group 《Obesity (Silver Spring, Md.)》2018,26(7):1203-1210
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Dawid Lewandowski Marta Lewandowska Piotr Ruszkowski Anita Pińska Grzegorz Schroeder 《PloS one》2015,10(5)
Novel zidovudine derivatives, able to be covalently conjugated to silica surface, have been obtained and grafted to SBA-15 mesoporous silica. Cytotoxic activity of the hybrid organic-inorganic (zidovudine derivatives-silica) systems against HeLa and KB cell lines has been analyzed. Addition of folic acid had a positive influence on the cytotoxicity. Up to 69% of HeLa and 65% of KB tumor cells growth inhibition has been achieved at low silica concentration used (10 μg/mL). 相似文献
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Juszczak K Kaszuba-Zwoińska J Chorobik P Ziomber A Thor PJ 《Cellular & molecular biology letters》2012,17(2):196-205
Highly concentrated urine may induce a harmful effect on the urinary bladder. Therefore, we considered osmolarity of the urine
as a basic pathomechanism of mucosal damage. The influence of both cyclophosphamide (CYP) and hyperosmolar stimuli (HS) on
the urothelium are not well described. The purpose was to evaluate the effect of CYP and HS on rat urothelial cultured cells
(RUCC). 15 Wistar rats were used for RUCC preparation. RUCC were exposed to HS (2080 and 3222 mOsm/l NaCl) for 15 min and
CYP (1 mg/ml) for 4 hrs. APC-labelled annexin V was used to quantitatively determine the percentage of apoptotic cells and
propidium iodide (PI) as a standard flow cytometric viability probe to distinguish necrotic cells from viable ones. Annexin
V-APC (+), annexin V-APC and PI (+), and PI (+) cells were analysed as apoptotic, dead, and necrotic cells, respectively.
The results were presented in percentage values. The flow cytometric analysis was done on a FACSCalibur Flow Cytometer using
Cell-Quest software. Treatment with 2080 and 3222 mOsm/l HS resulted in 23.7 ± 3.9% and 26.0 ± 1.5% apoptotic cells, respectively,
14.3 ± 1.4% and 19.4 ± 2.7% necrotic cells, respectively and 60.5 ± 1.4% and 48.6 ± 5.3% dead cells, respectively. The effect
of CYP on RUCC was similar to the effect of HS. After CYP the apoptotic and necrotic cells were 23.1 ± 0.3% and 17.9 ± 7.4%,
respectively. The percentage of dead cells was 57.7 ± 10.8%. CYP and HS induced apoptosis and necrosis in RUCC. 3222 mOsm/l
HS had the most harmful effect based on the percentage of necrotic and apoptotic cells. 相似文献