全文获取类型
收费全文 | 35539篇 |
免费 | 2946篇 |
国内免费 | 1882篇 |
出版年
2024年 | 51篇 |
2023年 | 336篇 |
2022年 | 844篇 |
2021年 | 1648篇 |
2020年 | 1039篇 |
2019年 | 1276篇 |
2018年 | 1246篇 |
2017年 | 883篇 |
2016年 | 1344篇 |
2015年 | 2071篇 |
2014年 | 2412篇 |
2013年 | 2588篇 |
2012年 | 3156篇 |
2011年 | 2804篇 |
2010年 | 1761篇 |
2009年 | 1470篇 |
2008年 | 1707篇 |
2007年 | 1553篇 |
2006年 | 1383篇 |
2005年 | 1174篇 |
2004年 | 1047篇 |
2003年 | 888篇 |
2002年 | 779篇 |
2001年 | 697篇 |
2000年 | 696篇 |
1999年 | 672篇 |
1998年 | 393篇 |
1997年 | 339篇 |
1996年 | 365篇 |
1995年 | 339篇 |
1994年 | 331篇 |
1993年 | 223篇 |
1992年 | 355篇 |
1991年 | 274篇 |
1990年 | 314篇 |
1989年 | 263篇 |
1988年 | 193篇 |
1987年 | 174篇 |
1986年 | 157篇 |
1985年 | 136篇 |
1984年 | 125篇 |
1983年 | 96篇 |
1982年 | 78篇 |
1981年 | 63篇 |
1979年 | 77篇 |
1978年 | 55篇 |
1977年 | 53篇 |
1975年 | 60篇 |
1974年 | 47篇 |
1973年 | 51篇 |
排序方式: 共有10000条查询结果,搜索用时 708 毫秒
91.
92.
93.
传入C纤维的兴奋在中电针“足三里”激活中缝大核中的作用 总被引:6,自引:0,他引:6
The purpose of the present work is to study whether the analgesia of "Zusanli" EA was mainly produced by its noxious effect. The antidromic C waves on N. peroneus communis innervating the area of "Zusanli" point were recorded. When "Zusanli" point was stimulated by trains of stimuli, the amplitude of the antidromic C wave was obviously decreased due to collision with the orthodromic stimulation. It was suggested that EA of "Zusanli" could excite some C fibers. It was observed that when the stimulation intensity reached the threshold of C fiber, the NRM neurons were obviously activated, and when it reached or exceeded the intensity for producing the maximal C wave, the NRM neurons were highly activated. Therefore, EA analgesia is probably produced mainly by its noxious stimulus component, especially carried by C fibers, via a negative feedback mechanism in modulating pain. 相似文献
94.
95.
DNA from Guthrie spots for diagnosis of DMD by multiplex PCR 总被引:2,自引:0,他引:2
E R McCabe Y Huang M Descartes Y H Zhang R G Fenwick 《Biochemical medicine and metabolic biology》1990,44(3):294-295
96.
Plants contain highly divergent actin isovariants 总被引:5,自引:0,他引:5
B G McLean S R Huang E C McKinney R B Meagher 《Cell motility and the cytoskeleton》1990,17(4):276-290
Actin protein isovariants have been identified in animals with distinct cytoplasmic or muscle specific patterns of expression. Analysis of vascular plant actin gene sequences suggests that an even greater diversity should exist within the plant actin protein families, but previous studies on plant proteins have not demonstrated the presence of multiple actin isovariants. Antibodies recognizing a conserved amino-terminal plant actin peptide, a family of plant actin peptides from a variable region, and two monoclonal antibodies to conserved epitopes within animal actins were used to identify isovariants of soybean actin resolved by two-dimensional isoelectric focusing (IEF) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Approximately six to eight actin isovariants with pI values ranging from 5.1 to 5.8 have been identified from soybean hypocotyls, stems, leaves, and roots with varying amounts of most isovariants present in all four organs. Acidic isovariants were present in much higher levels in leaves and stems. Antisera with lambda-class actin specificity detected a subset of three isovariants in all organs examined. One monoclonal and one antipeptide antisera are shown to react well with a wide variety of plant actin isovariants. Similar patterns of actin isovariants were detected in the distant angiosperms, Arabidopsis, petunia, and maize. It is likely that many of these diverse classes of isovariants have been preserved throughout vascular plant evolution and reflect the ancient diversity within plant actin gene families. The extreme difference among isovariants implies the presence of a complex actin-based cytoskeletal system in plants. 相似文献
97.
98.
D A Compton M M Weil L Bonetta A Huang C Jones H Yeger B R Williams L C Strong G F Saunders 《Genomics》1990,6(2):309-315
In a previous report, we described a contiguous restriction map of chromosome band 11p13 that localized the Wilms tumor locus to a small group of NotI fragments. In an effort to identify and isolate the 11p13-associated sporadic Wilms tumor locus, we developed a panel of NotI fragment-specific DNA probes. These probes were selected from genomic libraries constructed using the Chinese hamster ovary-human somatic cell hybrid carrying only human 11p. The libraries were prepared from NotI-digested DNA after size selection by pulsed-field gel electrophoresis. The selected NotI fragments had been previously targeted on the basis of deletion mapping as having a high probability of containing the Wilms tumor locus. We used these newly identified 11p13-specific probes to improve the resolution of the restriction map spanning the Wilms tumor locus. The locus has been defined by a homozygous deletion in a sporadic Wilms tumor. Using these probes, the region of homozygous deletion in this tumor and presumably all or part of the Wilms tumor gene have been confined to two small SfiI fragments spanning less than 350 kb. 相似文献
99.
Zinc has opposite effects on NMDA and non-NMDA receptors expressed in Xenopus oocytes 总被引:8,自引:0,他引:8
Pharmacological characterization of Zn2+ effects on glutamate ionotropic receptors was investigated in Xenopus oocytes injected with rat brain mRNA, using a double microelectrode, voltage-clamp technique. At low concentration, Zn2+ inhibited NMDA currents (IC50 = 42.9 +/- 1.3 microM) and potentiated both AMPA (EC50 = 30.0 +/- 1.2 microM) and desensitized kainate responses (EC50 = 13.0 +/- 0.1 microM). At higher concentrations, Zn2+ inhibited non-NMDA responses with IC50 values of 1.3 +/- 0.1 mM and 1.2 +/- 0.3 mM for AMPA and kainate, respectively. The potentiation of AMPA or quisqualate currents by Zn2+ was more than 2-fold, whereas that of the kainate current was only close to 30%. This potentiating effect of Zn2+ on AMPA current modified neither the affinity of the agonist for its site nor the current-voltage relationship. In addition, 500 microM Zn2+ differentially affected NMDA and non-NMDA components of the glutamate-induced response. The possible physiological relevance of Zn2+ modulation is discussed. 相似文献
100.
本文记述了在云南省建水县岔科地区发现的哺乳动物化石。根据豫鼠(Yuomys),定地层时代为晚始新世。这套地层暂称岔科组,以示滇南地区第一个含早第三纪哺乳动物的地点和层位。 相似文献