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901.
Activity of delta 5-3 beta-hydroxysteroid dehydrogenase coupled with steroid-delta 5-4-isomerase was demonstrated for the first time in the pancreas. The enzyme complex was assayed by measuring the conversion of pregnenolone to progesterone as well as of dehydroepiandrosterone to androstenedione and found to be localized primarily in the mitochondrial fraction of dog pancreas homogenates. The delta 5-3 beta-hydroxysteroid dehydrogenase used either NAD+ or NADP+ as co-substrates, although maximal activity was observed with NAD+. In phosphate buffer, pH 7.0 and 37 degrees C, the apparent Km values of the dehydrogenase were 6.54 +/- 0.7 microM for pregnenolone and 9.61 +/- 0.8 microM for NAD+. The apparent Vmax was determined as 0.82 +/- 0.02 nmol min-1 mg-1. Under the same conditions the Km values for dehydroepiandrosterone and NAD+ were 3.3 +/- 0.2 microM and 9.63 +/- 1.6 microM, respectively, and the apparent Vmax was 0.62 +/- 0.01 nmol min-1 mg-1. 相似文献
902.
903.
The photoperiodic variations of testosterone plasmatic levels in male rabbits have been studied. The animals were subjected to the influence of three circadian rhythms: 12/12, 14/10 and 10/14 h of light/darkness. The hormone assay was carried out using an enzymeimmunoassay method (Competitive ELISA). Blood samples were of two different types: seriated and non-seriated, to find if these might be any differences in the results. It is concluded that testosterone plasmatic levels are within physiologic values (0.3-10.0 ng/ml), although differences depending on the light/darkness cycle and on the type of blood extraction are remarkable; for this reason the selection of the circadian rhythm will depend on the experimentation that is to be carried out. 相似文献
904.
Olive tree tissues are able to metabolize ethylene. This metabolism is inhibited by heat killing and carbon disulfide. Rubber stoppers usually employed to close the incubation vials release carbon disulfide, thereby modifying the values obtained for ethylene. The ethylene consumption rate has been found to be 9 nl/h.g, an intermediate value among the different plant tissues so far examined. 相似文献
905.
C M Contreras R Garnica A Torres-Ruiz C Arenas M L Marván H Lara M Vázquez 《Boletín de estudios médicos y biológicos》1990,38(1-2):22-28
Variations in evoked potentials utilizing a photic stimulus in a sample of psychiatric patients compared to a healthy sample were evaluated. A group of patients diagnosed as schizophrenic was tested against a sample of healthy volunteers in a trial combining visual evoked potentials and a simultaneous cognitive processing. The stimulus was a checkerboard pattern presented under three different conditions. The results indicate diminished P100 and lack the reactivity associated with cognitive processes in schizophrenic group. The P200 component also lacked, in the inpatient group the changes associated with the performance of the trial. Finally the multiple P300 component was shortened in latency and decreased in amplitude in the schizophrenia group. Besides, P300 interhemispheric shifts related to trials, were commonly inverted in schizophrenics. Results are interpreted as a lacked interhemispheric coordination in schizophrenics, rather than a fixed hemispheric alteration. Likewise, an attenuation in processing from specific cortical areas to association cortex is concluded. 相似文献
906.
C Amaro A E Toranzo E A González J Blanco M J Pujalte R Aznar E Garay 《Applied and environmental microbiology》1990,56(4):1140-1147
A total of 140 environmental Vibrio cholerae non-O1 isolates, together with several culture collection strains from both environmental and clinical sources, were studied in relation to hemagglutination, surface hydrophobicity, and the enzymatic, hemolytic, cytotoxic, and enterotoxic activities of their extracellular products. A total of 78 and 62% of the strains produced hemagglutinins and exohemagglutinins, respectively. Four different hemagglutinating and two exohemagglutinating activities were found by using eight sugars in the inhibition assays. Cell-bound mannose-sensitive hemagglutination was detected mainly in chicken blood, whereas fucose-sensitive hemagglutination was recorded only in human blood. Cell-bound hemagglutinin resistant to all sugars tested was the only one related to surface hydrophobicity. The surface properties varied along the growth curves. The non-O1 strains displayed strong enzymatic and hemolytic activities, except for esculin hydrolysis. Of 26 non-O1 isolates selected for cytotoxin and enterotoxin production, 23 showed a wide spectrum of cytotoxic effects on cell lines of poikilothermic and homoiothermic species, but they were weakly enterotoxigenic in the infant mouse test. All extracellular products of cytotoxic strains were proteolytic, lipolytic, and hemolytic, and a high percentage produced hemagglutination of chicken blood. The cytotoxic factors in the non-O1 strains analyzed were not R plasmid mediated. 相似文献
907.
Silastic devices impregnated with oestradiol and blank devices were placed around both oviducts and around skeletal muscle bundles in the forelegs to attain local and systematic delivery, respectively. Another group of mice received an oestradiol-impregnated device around one oviduct and a blank device in the contralateral oviduct. Implantation of blank devices around the oviducts and in the forelegs did not alter ovum transport. Devices impregnated with oestradiol placed around both oviducts produced a dose-dependent delay of ovum transport, which was more pronounced than the effect of devices located in the forelegs. Oviducts receiving an oestradiol-loaded device had a larger retention of ova than did the contralateral oviducts receiving a blank device. These results demonstrate a direct action of oestradiol upon the oviduct to delay ovum transport in the mouse. 相似文献
908.
The experimentally reported kinetic behaviour (sub-exponential but supra-linear growth) of non-enzymatic template replication is incorporated into a simple model of template competition. Sub-exponential growth is shown to lead to coexistence invariably. Thus coexistence of different non-enzymatically replicating sequences is predicted. This type of coexistence could have been important in maintaining a sufficient diversity of RNA modules used later to build functional molecules such as ribozymes. Experimental tests of this theoretical prediction are possible. 相似文献
909.
The effect of a random initial value is examined in several stochastic integrate-and-fire neural models with a constant threshold and a constant input. The three models considered are approximations of Stein's model, namely: (1) a leaky integrator with deterministic trajectories, (2) a Wiener process with drift, and (3) an Ornstein-Uhlenbeck process. For model 1, different distributions for the initial value lead to commonly observed interspike interval distributions. For model 2, a discrete and a uniform distribution for the initial value are examined along with some parameter estimation procedures. For model 3, with a truncated normal distribution for the initial value, the coefficient of variation is shown to be greater than 1, and as the threshold becomes large the first-passage-time distribution approaches an exponential distribution. The relationships among the models and between them and previous models are also discussed, along with the robustness of the model assumptions and methods of their verification. The effects of a random initial value are found to be most pronounced at high firing rates. 相似文献
910.
G Horváth 《Mathematical biosciences》1989,96(1):79-94
A general method is given to determine theoretically the shape of the aspherical interface that eliminates spherical aberration in an optionally shaped thick lens. The theory is applied to trilobite eyes. On the basis of the geometric optical method presented, the refractive indices and focal length of the original corneal lenses of trilobites can be determined. The shape of the aspherical interface in the cornea of some phacopid trilobites with schizochroal eyes is investigated. The theoretical aspherical interfaces agree well with the real ones. 相似文献