全文获取类型
收费全文 | 3549篇 |
免费 | 342篇 |
出版年
2023年 | 17篇 |
2022年 | 22篇 |
2021年 | 51篇 |
2020年 | 31篇 |
2019年 | 61篇 |
2018年 | 56篇 |
2017年 | 72篇 |
2016年 | 120篇 |
2015年 | 187篇 |
2014年 | 209篇 |
2013年 | 233篇 |
2012年 | 252篇 |
2011年 | 230篇 |
2010年 | 149篇 |
2009年 | 139篇 |
2008年 | 190篇 |
2007年 | 155篇 |
2006年 | 149篇 |
2005年 | 131篇 |
2004年 | 127篇 |
2003年 | 132篇 |
2002年 | 122篇 |
2001年 | 54篇 |
2000年 | 64篇 |
1999年 | 71篇 |
1998年 | 52篇 |
1997年 | 40篇 |
1996年 | 55篇 |
1995年 | 45篇 |
1994年 | 42篇 |
1993年 | 34篇 |
1992年 | 60篇 |
1991年 | 32篇 |
1990年 | 48篇 |
1989年 | 34篇 |
1988年 | 36篇 |
1987年 | 35篇 |
1986年 | 34篇 |
1985年 | 30篇 |
1984年 | 38篇 |
1983年 | 21篇 |
1982年 | 27篇 |
1981年 | 22篇 |
1980年 | 19篇 |
1979年 | 21篇 |
1977年 | 17篇 |
1976年 | 17篇 |
1974年 | 15篇 |
1973年 | 17篇 |
1972年 | 16篇 |
排序方式: 共有3891条查询结果,搜索用时 31 毫秒
111.
The synergistic effect of exogenous cobra phospholipase A2 on the hemolysis rate of guinea pig erythrocytes by highly purified snake venom cardiotoxins was investigated. In the presence of phospholipase A2 the reaction was not only faster and had a lower activation energy but followed a sigmoidal instead of a linear time course. Similar results were obtained using porcine pancreatic phospholipase A2. Significantly, addition of even a trace of cobra phospholipase A2 (approx. 0.1%, w/w) was sufficient to bring about the full synergistic effect, emphasizing the stringent purity requirements for any meaningful investigation of cardiotoxin's own action. The possibility that the action of cardiotoxin on its own may involve the stimulation of an endogenous phospholipase is discussed in the light of the results obtained with exogenous cobra enzyme. 相似文献
112.
113.
The relation between cell proliferation,differentiation and ultrastructural development in rat intestinal epithelium 总被引:4,自引:0,他引:4
Dr. J. M. van Dongen W. J. Visser W. Th. Daems H. Galjaard 《Cell and tissue research》1976,174(2):183-199
Summary The ultrastructural development of the principal cells in rat small intestine was studied by morphometric analyses in relation to the exact cell position along crypt and villus. From the bottom to the tip of the crypt, a gradual increase occurred in absolute size of the total cell, the cytoplasm, the terminal web and of nearly all cell organelles. Also, the relative size of the cytoplasm, mitochondria, microvilli and endoplasmic reticulum increased during crypt cell differentiation. No sudden changes in ultrastructure were observed in the so-called critical decision zone, normally located halfway up the crypt where the proliferative activity ceases. At the crypt-villous junction a 1.4–3 fold increase in cell size, cytoplasm, terminal web and of most organelles was noted. Expansion of the proliferative cell compartment over the total length of the crypt as occurs during recovery after a low X-irradiation dose (72 h after 400 R) does not affect the normal development of cellular ultrastructure. These findings are discussed in relation to biochemical and cell kinetic data. 相似文献
114.
A selective method for the isolation of Aspergillus nidulans mutants defective in the pyruvate dehydrogenase complex was devised. The essential steps in the procedure were a mutagenic treatment of conidia with X-rays to about 50% survival, followed by filtration enrichment in minimal medium with D-galacturonate as sole carbon source, and rescue on complete medium with acetate. The mutants thus isolated were phenotypically characterized on the basis of growth tests, and different genotypes were assigned on the basis of complementation tests. The majority of the mutants that were unable to utilize galacturonate were defective in one of the components of the pyruvate dehydrogenase complex. In addition, mutants defective in pyruvate carboxylase, mutants defective in glycerol catabolism and some novel mutants which were only unable to use D-galacturonate as carbon source were found. At least two genes were shown to be involved in D-galacturonate metabolism. 相似文献
115.
116.
Jeroen de Ridder Pieter Meysman Olugbenga Oluwagbemi Thomas Abeel 《PLoS computational biology》2014,10(7)
Contributing to a student organization, such as the International Society for Computational Biology Student Council (ISCB-SC) and its Regional Student Group (RSG) program, takes time and energy. Both are scarce commodities, especially when you are trying to find your place in the world of computational biology as a graduate student. It comes as no surprise that organizing ISCB-SC-related activities sometimes interferes with day-to-day research and shakes up your priority list. However, we unanimously agree that the rewards, both in the short as well as the long term, make the time spent on these extracurricular activities more than worth it. In this article, we will explain what makes this so worthwhile: soft skills. 相似文献
117.
118.
119.
James I. Kirkland Luis Alcalá Mark A. Loewen Eduardo Espílez Luis Mampel Jelle P. Wiersma 《PloS one》2013,8(12)
Nodosaurids are poorly known from the Lower Cretaceous of Europe. Two associated ankylosaur skeletons excavated from the lower Albian carbonaceous member of the Escucha Formation near Ariño in northeastern Teruel, Spain reveal nearly all the diagnostic recognized character that define nodosaurid ankylosaurs. These new specimens comprise a new genus and species of nodosaurid ankylosaur and represent the single most complete taxon of ankylosaur from the Cretaceous of Europe. These two specimens were examined and compared to all other known ankylosaurs. Comparisons of these specimens document that Europelta carbonensis n. gen., n. sp. is a nodosaur and is the sister taxon to the Late Cretaceous nodosaurids Anoplosaurus, Hungarosaurus, and Struthiosaurus, defining a monophyletic clade of European nodosaurids– the Struthiosaurinae. 相似文献
120.
Marijn Bauters Sofie Meeus Matti Barthel Piet Stoffelen Hannes P. T. De Deurwaerder Flicien Meunier Travis W. Drake Quentin Ponette Jerme Ebuy Pieter Vermeir Hans Beeckman Francis wyffels Samuel Bod Hans Verbeeck Filip Vandelook Pascal Boeckx 《Global Change Biology》2020,26(8):4449-4461
Forests exhibit leaf‐ and ecosystem‐level responses to environmental changes. Specifically, rising carbon dioxide (CO2) levels over the past century are expected to have increased the intrinsic water‐use efficiency (iWUE) of tropical trees while the ecosystem is gradually pushed into progressive nutrient limitation. Due to the long‐term character of these changes, however, observational datasets to validate both paradigms are limited in space and time. In this study, we used a unique herbarium record to go back nearly a century and show that despite the rise in CO2 concentrations, iWUE has decreased in central African tropical trees in the Congo Basin. Although we find evidence that points to leaf‐level adaptation to increasing CO2—that is, increasing photosynthesis‐related nutrients and decreasing maximum stomatal conductance, a decrease in leaf δ13C clearly indicates a decreasing iWUE over time. Additionally, the stoichiometric carbon to nitrogen and nitrogen to phosphorus ratios in the leaves show no sign of progressive nutrient limitation as they have remained constant since 1938, which suggests that nutrients have not increasingly limited productivity in this biome. Altogether, the data suggest that other environmental factors, such as increasing temperature, might have negatively affected net photosynthesis and consequently downregulated the iWUE. Results from this study reveal that the second largest tropical forest on Earth has responded differently to recent environmental changes than expected, highlighting the need for further on‐ground monitoring in the Congo Basin. 相似文献