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81.
Using Satellite Remote Sensing to Estimate the Colored Dissolved Organic Matter Absorption Coefficient in Lakes 总被引:4,自引:0,他引:4
Tiit Kutser Donald C. Pierson Lars Tranvik Anu Reinart Sebastian Sobek Kari Kallio 《Ecosystems》2005,8(6):709-720
Given the importance of colored dissolved organic matter (CDOM) for the structure and function of lake ecosystems, a method
that could estimate the amount of CDOM in lake waters over large geographic areas would be highly desirable. Satellite remote
sensing has the potential to resolve this problem. We carried out model simulations to evaluate the suitability of different
satellite sensors (Landsat, IKONOS, and the Advanced land Imager [ALI]) to map the amount of CDOM in concentration ranges
that occur in boreal lakes of the Nordic countries. The results showed that the 8-bit radiometric resolution of Landsat 7
is not adequate when absorption by CDOM at 420 nm is higher than 3 m−1. On the other hand, the 16-bit radiometric resolution of ALI, a prototype of the next generation of Landsat, is suitable
for mapping CDOM in a wider range of concentrations. An ALI image of southern Finland was acquired on 14, July 2002 and in
situ measurements were carried out in 15 lakes (18 stations). The results showed that there is a high correlation (R2 = 0.84) between the 565 nm/660 nm ALI band ratio and the CDOM absorption coefficient in lakes. Analysis of 245 lakes in the
acquired satellite image showed a normal distribution of CDOM concentration among the lakes. However, the size distribution
of lakes was highly skewed toward small lakes, resulting in the CDOM concentration per unit lake area being skewed toward
high values. We showed that remote sensing enables synoptic monitoring of the CDOM concentration in a large number of lakes
and thus enables scaling up to the level of large ecosystems and biomes. 相似文献
82.
Cheryl A. Nickerson C. Mark Ott James W. Wilson Rajee Ramamurthy Duane L. Pierson 《Microbiological reviews》2004,68(2):345-361
Microbial adaptation to environmental stimuli is essential for survival. While several of these stimuli have been studied in detail, recent studies have demonstrated an important role for a novel environmental parameter in which microgravity and the low fluid shear dynamics associated with microgravity globally regulate microbial gene expression, physiology, and pathogenesis. In addition to analyzing fundamental questions about microbial responses to spaceflight, these studies have demonstrated important applications for microbial responses to a ground-based, low-shear stress environment similar to that encountered during spaceflight. Moreover, the low-shear growth environment sensed by microbes during microgravity of spaceflight and during ground-based microgravity analogue culture is relevant to those encountered during their natural life cycles on Earth. While no mechanism has been clearly defined to explain how the mechanical force of fluid shear transmits intracellular signals to microbial cells at the molecular level, the fact that cross talk exists between microbial signal transduction systems holds intriguing possibilities that future studies might reveal common mechanotransduction themes between these systems and those used to sense and respond to low-shear stress and changes in gravitation forces. The study of microbial mechanotransduction may identify common conserved mechanisms used by cells to perceive changes in mechanical and/or physical forces, and it has the potential to provide valuable insight for understanding mechanosensing mechanisms in higher organisms. This review summarizes recent and future research trends aimed at understanding the dynamic effects of changes in the mechanical forces that occur in microgravity and other low-shear environments on a wide variety of important microbial parameters. 相似文献
83.
Negative Cross-Communication among Wheat Rhizosphere Bacteria: Effect on Antibiotic Production by the Biological Control Bacterium Pseudomonas aureofaciens 30-84
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Phenazine antibiotic production in the biological control bacterium Pseudomonas aureofaciens 30-84 is regulated in part via the PhzR/PhzI N-acyl homoserine lactone (AHL) system. Previous work showed that a subpopulation of the wheat rhizosphere community positively affected phenazine gene expression in strain 30-84 via AHL signals (E. A. Pierson, D. W. Wood, J. A. Cannon, F. M. Blachere, and L. S. Pierson III, Mol. Plant-Microbe Interact. 11:1078-1084, 1998). In the present work, a second subpopulation, one that negatively affected phenazine gene expression, was identified from this rhizosphere community. Strain 30-84 grown in conditioned medium (CM) from several strains produced lower levels of phenazines (1.5- to 9.3-fold) than control when grown in CM from the strain 30-84I1/I2. Growth of the phzB::lacZ reporter strain 30-84Z in this CM resulted in decreased lacZ expression (4.3- to 9.2-fold) compared to growth of the control strain in CM, indicating that inhibition of phzB occurred at the level of gene expression. Preliminary chemical and biological characterizations suggested that these signals, unlike other identified negative signals, were not extractable in ethyl acetate. Introduction of extra copies of phzR and phzI, but not phzI alone, in trans into strain 30-84Z reduced the negative effect on phzB::lacZ expression. The presence of negative-signal-producing strains in a mixture with strain 30-84 reduced strain 30-84's ability to inhibit the take-all disease pathogen in vitro. Together, the results from the previous work on the positive-signal subpopulation and the present work on the negative-signal subpopulation suggest that cross-communication among members of the rhizosphere community and strain 30-84 may control secondary metabolite production and pathogen inhibition. 相似文献
84.
85.
Molecular Characterization of Novel Red Green Nonsulfur Bacteria from Five Distinct Hot Spring Communities in Yellowstone National Park 总被引:5,自引:3,他引:2
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Sarah M. Boomer Daniel P. Lodge Bryan E. Dutton Beverly Pierson 《Applied microbiology》2002,68(1):346-355
We characterized and compared five geographically isolated hot springs with distinct red-layer communities in Yellowstone National Park. Individual red-layer communities were observed to thrive in temperatures ranging from 35 to 60°C and at pH 7 to 9. All communities were dominated by red filamentous bacteria and contained bacteriochlorophyll a (Bchl a), suggesting that they represented novel green nonsulfur (GNS) bacteria. The in vivo absorption spectra of individual sites were different, with two sites showing unusual Bchl a protein absorption bands beyond 900 nm. We prepared and analyzed 16S rRNA libraries from all of these sites by using a combination of general bacterial primers and new GNS-specific primers described here. These studies confirmed the presence of novel GNS-like bacteria in all five communities. All GNS-like clones were most similar to Roseiflexus castenholzii, a red filamentous bacterium from Japan that also contains only Bchl a. Phylogenies constructed by using GNS-like clones from Yellowstone red-layer communities suggest the presence of a moderately diverse new “red” cluster within the GNS lineage. Within this cluster, at least two well-supported subclusters emerged: YRL-A was most similar to Roseiflexus and YRL-B appeared to be novel, containing no known isolates. While these patterns showed some site specificity, they did not correlate with observed Bchl a spectrum differences or obvious features of the habitat. 相似文献
86.
Jan Derksen Bart Knuiman Karin Hoedemaekers Anouchka Guyon Sandrine Bonhomme Elisabeth S. Pierson 《Sexual plant reproduction》2002,15(3):133-139
Tricellular pollen tubes of Arabidopsis thaliana were cultured in vitro on solid media and studied with respect to growth, cellular organization and ultrastructure, cytoskeletal organization, organelle movement, deposition and structure of the wall and the occurrence of coated pits, all elements assumed to be relevant for tip growth. For our ultrastructural studies we used freeze fixation and freeze substitution. Although Arabidopsis pollen tubes are broadly similar to those of bicellular species such as Nicotiana tabacum and Lilium spec. and in vivo grown pollen tubes of Arabidopsis, some differences occurred. The density of the equally distributed, relatively small (85 nm) secretory vesicles (SV) in the tip is low (five/µm 2). In between the SV of the tip, membranous material, possibly smooth endoplasmic reticulum, fragments of rough endoplasmic reticulum and loose ribosomes are present. The wall in the tip is not amorphous but layered and a secondary wall is formed already in the flanks of the tip. The general pattern of organelle motion is reverse fountain-like, but individual organelles move in distinct lanes at speeds of up to 2 µm/s, and about half of the organelle population shows a moderate velocity or Brownian movement. These properties are discussed in relation to the low growth rate (10 µm/h) of Arabidopsis pollen grown in vitro. The two similar sperm cells are closely attached and are always found near the vegetative nucleus. No surrounding wall and no cytoskeletal elements were obvious in the sperm cells. The preferential location of the mitochondria at the wall and the large (up to 400 nm) coated pits are unique for angiosperm pollen tubes. The size of the coated pits may allow not only membrane retrieval but also pinocytosis. 相似文献
87.
Classical decision theory predicts that people should be indifferent to information that is not useful for making decisions, but this model often fails to describe human behavior. Here we investigate one such scenario, where people desire information about whether an event (the gain/loss of money) will occur even though there is no obvious decision to be made on the basis of this information. We find a curious dual trend: if information is costless, as the probability of the event increases people want the information more; if information is not costless, people''s desire for the information peaks at an intermediate probability. People also want information more as the importance of the event increases, and less as the cost of the information increases. We propose a model that explains these results, based on the assumption that people have limited cognitive resources and obtain information about which events will occur so they can determine whether to expend effort planning for them. 相似文献
88.
Schoene R. B.; Bates P. W.; Larson E. B.; Pierson D. J. 《Journal of applied physiology》1983,55(6):1772-1776
89.
Beverly K. Pierson 《Photosynthesis research》1994,41(1):7-15
A personal historic account is presented of the discovery of the anoxygenic filamentous bacteria of hot springs:Heliothrix oregonensis andChloroflexus aurantiacus. The later discoveries of marine and hypersaline forms are also described. 相似文献
90.
In a continuing study of microbial secondary metabolism in
simulated microgravity, we have examined gramicidin S (GS) production by
Bacillus brevis strain Nagano in NASA High Aspect Rotating Vessels
(HARVs), which are designed to simulate some aspects of microgravity. Growth
and GS production were found to occur under simulated microgravity. When
performance under simulated microgravity was compared with that under normal
gravity conditions in the bioreactors, GS production was found to be
unaffected by simulated microgravity. The repressive effect of glycerol in
flask fermentations was not observed in the HARV. Thus the negative effect of
glycerol on specific GS formation is dependent on shear and/or vessel
geometry, not gravity.
Received: 7 August 1996 / Accepted: 17 September 1996 相似文献