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992.
This study examined the reproduction and population structure of the blue-spotted maskray Neotrygon kuhlii within Moreton Bay, a subtropical embayment in south-east Queensland, Australia. Mean sizes at maturity were 314 mm disc width ( W D ) in females ( n = 140, 115–465 mm W D ) and 294 mm W D in males ( n = 123, 129–381 mm W D ). Female N. kuhlii had a synchronous annual reproductive cycle, with one litter of one to three pups (mean ± s.d . 1·67 ± 0·71) produced per year. Mating behaviours were observed in October and November, and ovulation occurred early in the Austral summer, overlapping with the start of embryonic development. Gestation took c. 4 months with parturition occurring in late February and March. Size at birth was 115–170 mm W D . The population showed a significant female bias, particularly in larger size classes. Tagging studies produced a total recapture rate of 16·1% and indicated that N. kuhlii were site resident for up to 1081 days. 相似文献
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Below-ground carbon dioxide (CO2) emissions occur naturally at CO2 springs, but the risk of occurrence at other sites will increase as geologic CO2 storage is implemented to help mitigate climate change. This investigation examines the effects of elevated soil CO2 concentrations from such emissions on vegetation biomass and microbial community biomass, respiration and carbon utilisation in temperate grassland. Soil CO2 concentrations was increased by release of concentrated CO2 gas from a point source 0.6 m below the surface of the soil as a low-level leak (1 l min?1) for 10 weeks. The gassing resulted in reduced vegetation above- and below-ground biomass over time. No significant changes in microbial biomass or carbon utilisation were observed, but a trend towards reduced microbial respiration was apparent. This research provides a first step towards understanding the potential ecological risks of geologic carbon storage, the development of biological leak detection methods, and improved understanding of the effects of elevated soil CO2 concentrations on biological communities. 相似文献
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SPECIALIZATION AND GEOGRAPHIC ISOLATION AMONG WOLBACHIA SYMBIONTS FROM ANTS AND LYCAENID BUTTERFLIES
Jacob A. Russell Benjamin Goldman-Huertas Corrie S. Moreau Laura Baldo Julie K. Stahlhut John H. Werren Naomi E. Pierce 《Evolution; international journal of organic evolution》2009,63(3):624-640
Wolbachia are the most prevalent and influential bacteria described among the insects to date. But despite their significance, we lack an understanding of their evolutionary histories. To describe the evolution of symbioses between Wolbachia and their hosts, we surveyed global collections of two diverse families of insects, the ants and lycaenid butterflies. In total, 54 Wolbachia isolates were typed using a Multi Locus Sequence Typing (MLST) approach, in which five unlinked loci were sequenced and analyzed to decipher evolutionary patterns. AMOVA and phylogenetic analyses demonstrated that related Wolbachia commonly infect related hosts, revealing a pattern of host association that was strongest among strains from the ants. A review of the literature indicated that horizontal transfer is most successful when Wolbachia move between related hosts, suggesting that patterns of host association are driven by specialization on a common physiological background. Aside from providing the broadest and strongest evidence to date for Wolbachia specialization, our findings also reveal that strains from New World ants differ markedly from those in ants from other locations. We, therefore, conclude that both geographic and phylogenetic barriers have promoted evolutionary divergence among these influential symbionts. 相似文献
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A mutation in Epstein-Barr virus LMP2A reveals a role for phospholipase D in B-Cell antigen receptor trafficking 总被引:1,自引:0,他引:1
Epstein-Barr virus (EBV) latent infection of B cells blocks the interrelated signaling and antigen-trafficking functions of the BCR through the activity of its latent membrane protein 2A (LMP2A). At present, the molecular mechanisms by which LMP2A exerts its control of BCR functions are only poorly understood. Earlier studies showed that in B cells expressing LMP2A containing a tyrosine mutation at position 112 in its cytoplasmic domain (Y112-LMP2A), the BCR could initiate signaling but could not properly traffic antigen for processing. Here, we show that BCR signaling in Y112-LMP2A-expressing cells is attenuated with a reduction in both the degree and duration of phosphorylation of key components of the BCR signaling cascade including Syk, BLNK, PI3K, and Btk. Notably, Y112-LMP2A expression completely blocked the BCR-induced activation of phospholipase D (PLD), a lipase implicated in the intracellular trafficking of a variety of surface receptors. We show that blocking PLD activity, by expressing Y112-LMP2A, treating cells with the PLD inhibitor 1-butanol or reducing PLD expression by siRNA, blocked BCR trafficking to class II-containing compartments. Moreover, Y112-LMP2A expression blocked the recruitment of phosphorylated forms of the downstream BCR signaling components, Erk and JNK, through both PLD-dependent and PLD-independent mechanisms. Thus, the investigation of the mechanism by which Y112-LMP2A blocks BCR function revealed an essential role for PLD in BCR trafficking for antigen processing. 相似文献
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Sandra S. Wise Amie L. Holmes John Pierce Wise Sr. 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2006,610(1-2):2
Particulate hexavalent chromium (Cr(VI)) is a well-established human lung carcinogen. It is currently a major public health concern, there is widespread exposure to it in occupational settings and to the general public. However, despite the potential widespread exposure and the fact that the lung is its target organ, few studies have considered the toxic effects of particulate Cr(VI) in human lung cells. Accordingly, we used lead chromate as a model particulate Cr(VI) compound and determined its cytotoxicity and genotoxicity in cultured human bronchial epithelial cells, using BEP2D cells as a model cell line. We found that lead chromate induced concentration-dependent cytotoxicity in BEP2D cells after a 24 h exposure. Specifically, the relative survival was 78, 59, 53, 46 and 0% after exposure to 0.5, 1, 5, 10 and 50 μg/cm2 lead chromate, respectively. Similarly, the amount of chromosome damage increased with concentration after 24 h exposure to lead chromate. Specifically, 0.5, 1, 5 and 10 μg/cm2 damaged 10, 13, 20 and 28% of metaphase cells with the total amount of damage reaching 11, 15, 24 and 36 aberrations per 100 metaphases, respectively. Lead chromate (50 μg/cm2 lead chromate) induced profound cell cycle delay and no metaphases were found. In addition we investigated the effects of soluble hexavalent chromium, sodium chromate, in this cell line. We found that 1, 2.5, 5 and 10 μM sodium chromate induced 66, 35, 0 and 0% relative survival, respectively. The amount of chromosome damage increased with concentration after 24 h exposure to sodium chromate. Specifically, 1, 2.5 and 5 μM damaged 25, 34 and 41% of metaphase cells with the total amount of damage reaching 33, 59 and 70 aberrations per 100 metaphases, respectively. Ten micromolar sodium chromate induced profound cell cycle delay and no metaphases were found. Overall the data clearly indicate that hexavalent Cr(VI) is cytotoxic and genotoxic to human lung epithelial cells. 相似文献
1000.
Eliza Grlickova-Duzevik Sandra S. Wise Ray C. Munroe W. Douglas Thompson John Pierce Wise Sr. 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2006,610(1-2):31
Hexavalent chromium Cr(VI) is a well known human carcinogen. This genotoxic metal induces DNA strand breaks and chromosome damage. However, the relationship between these lesions is uncertain. Our study focused on examining the role of XRCC1 in sodium chromate-induced cytotoxicity and chromosomal aberrations in Chinese Hamster Ovary (CHO) cells. Three different cell lines were used: AA8 (parental), EM9 (XRCC1 mutant) and H9T3 (EM9 complemented with human XRCC1 gene). Results show that concentration-dependent decreases in relative survival are similar in all three cell lines, indicating that XRCC1 is not crucial for protecting cells from sodium chromate-induced cytotoxicity. Similarly the frequency of damaged metaphase cells was not affected by XRCC1 deficiency. However, the total number of Cr(VI)-induced chromosome aberrations was exacerbated by XRCC1 deficiency and the spectrum of chromosome damage changed dramatically. Specifically, chromatid and isochromatid lesions were the most prominent aberrations induced in the cell lines and XRCC1 was essential to reduce the formation of chromatid lesions. In addition, XRCC1 deficiency caused a dramatic increase in the number of chromatid exchanges indicating that it is involved in protection from Cr(VI)-induced chromosome instability. 相似文献