Further evidence for heterogeneity of glucose-6-phosphate dehydrogenase deficiency in Papua New Guinea

Summary Four new G6PD variants have been characterized in individuals from Papua New Guinea. This study demonstrates that the previously reported Markham variant and the newly characterized Salata variant may be widely distributed in Papua New Guinea. The data presented here together with those of previously published studies demonstrate a degree of heterogeneity of G6PD deficiency that is much higher than that in other regions of the world where G6PD deficiency is common.     

Genetic Variability in Taro, Colocasia esculenta (L.) Schott (Araceae)

Tablés 1 to 3 were omitted from the final printing ofthis article. They are reprinted here.     

Separation of metabolically radiolabeled O-methylmannitols and O-methylfucitol by reverse-phase high-performance liquid chromatography: applications to animal cell oligosaccharide structural analysis

Reverse-phase high-performance liquid chromatography was utilized to separate efficiently and rapidly a standard mixture of various radiolabeled O-methylated mannitols and O-methylfucitol commonly encountered when vertebrate asparagine-linked oligosaccharides are subjected to permethylation, hydrolysis, and reduction with NaBH4. The following reduced, radioactive O-methylhexitols were resolved: 2,4-, 3,4-, and 3,6-di-O-methylmannitols; 3,4,6-tri-O-methylmannitol, 2,3,4-tri-O-methylfucitol, and 2,3,4,6-tetra-O-methylmannitol. To demonstrate the utility of this separation method in the analysis of metabolically radiolabeled asparagine-linked oligosaccharides, mouse lymphoma BW 5147 cells were metabolically radiolabeled with [2-3H]mannose and their glycopeptides prepared by Pronase digestion and fractionated by serial chromatography on immobilized lectins. Each fraction was subjected to methylation and hydrolysis, the released monosaccharides were reduced, and the radioactive O-methylhexitols were separated by reverse-phase HPLC. The relative amounts of the O-methylhexitols in each glycopeptide fraction analyzed were similar to those values determined by a combination of other separation systems.     

Identification of Phytopathogenic Virus Strains by their Luminescent Properties

A quick test has been developed to identify phytopathogenic virus strains by variations in their photoluminescence parameters. When heated, the virus suspension shows a jumpwise drop or rise in fluorescence intensity caused by conformational changes in the macromolecules of the virion protein capsules. The relative value of the jump in fluorescence intensity and the temperature value at which the jump occurs are essentially strain-specific. The minimum time needed for one test is from 15 to 20 minutes.     

Germination of the chlamydospores of Candida albicans

Using a slide culture technique, it was determined unequivocally that the chlamydospore of Candida albicans does germinate. The germination was by way of a multiple-budding process which was associated mostly with young spores following their transfer to a fresh environment. It is concluded the chlamydospore of C. albicans is a reproductive cell with a transient viability.