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211.
Hypoxia tolerance in Atlantic cod 总被引:2,自引:0,他引:2
Oxygen saturation levels that killed 50 and 5% of cod Gadus morhua over 96 h averaged 21·2 and 27·7%, respectively. No fish survived at 10% saturation and only a few survived at 16% saturation, whereas no mortality occurred at 34 and 40% oxygen saturation. Since metabolic rate and oxygen consumption increase with increasing temperature, we hypothesized that cod would be less tolerant to hypoxic conditions at 6 than at 2° C. However, temperature (2 and 6° C) had no measurable impact on cod survival. Small (mean & S.D.; 45·2 ± 4·2 cm) and large (57·5 ± 3·8 cm) cod had the same tolerance to hypoxia. At the end of the experiments, hypoxia had a significant effect on blood haematocrit, mean cellular haemoglobin content, liver lactate, plasma glucose and plasma lactate, but accounted for only a small fraction (< 10%) of the variation, except for plasma lactate which exhibited a strong response with concentrations increasing progressively with decreasing levels of oxygen saturation. Temperature had a significant effect on most variates in normoxia and hypoxia. Variates also affected by oxygen level showed significant interactions between oxygen and size or temperature effects. However, these interactions accounted for only a small proportion of the variation. Physiological parameters indicated that extending the duration of our tests beyond 96 h would not have changed our estimates of the lethal thresholds. Hypoxic conditions are a permanent feature of the deep waters of the Gulf of St Lawrence. This study shows that a significant portion of the benthic habitats in the Gulf are uninhabitable for cod which would be expected to avoid waters below 28% oxygen saturation. 相似文献
212.
S M Schmutz J M Stookey D C Winkelman-Sim C S Waltz Y Plante F C Buchanan 《The Journal of heredity》2001,92(3):290-292
Two behavioral traits, temperament and habituation, were measured in 130 calves from 17 full-sib families which comprise the Canadian Beef Cattle Reference Herd. Using variance components, heritability was calculated as 0.36 for temperament and 0.46 for habituation. Genotyping of 162 microsatellites at approximately 20 cM intervals allowed the detection of six quantitative trait loci (QTL) for behavior traits on cattle chromosomes 1, 5, 9, 11, 14, 15. 相似文献
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The continuous infusion or bolus injection of the platelet-activating factor (PAF) is associated with profound hypotension, marked reductions of renal plasma flow, glomerular filtration, and urinary sodium excretion. All these effects are inhibited by blocking PAF receptors. To examine further the potential mediators of PAF on renal function, we utilized L-655,240 (6 mg/kg, intravenously), a thromboxane-prostaglandin endoperoxide antagonist, to study the systemic and renal response to PAF (0.8 micrograms/kg, intravenously) in the anesthetized dog, using clearance methodology. PAF decreased blood pressure from 115 +/- 7 to 54 +/- 4 mmHg (1 mmHg = 133.3 Pa), renal plasma flow from 105 +/- 6 to 74 +/- 56 mL/min, and glomerular filtration from 43 +/- 3 to 32 +/- 1 mL/min. PAF also reduced urine volume from 1.1 +/- 0.2 to 0.4 +/- 0.1 mL/min, and urinary sodium from 158 +/- 7 to 86 +/- 7 mu equiv./min. L-655,240 alone had no significant effect on blood pressure, renal plasma flow, and filtration rate, at any dose. However, the 6-mg/kg dose resulted in a slight elevation of diuresis, from 1.1 +/- 0.2 to 1.9 +/- 0.1 mL/min, and urinary sodium, from 134 +/- 13 to 212 +/- 19 mu equiv./min. All doses of L-655,240 blocked the effect of PAF on blood pressure. However, the two lower doses of this antagonist (1 and 3 mg/kg) failed to prevent the PAF-induced fall of renal plasma flow and filtration rate, and attenuated the effect on urinary sodium in a dose-dependent manner.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
215.
A turbidimetric technique was used to measure the bacteriolytic activities of digestive fluids collected seasonally from the deposit-feeding polychaete Arenicola marina. Lysis of cultured sedimentary bacteria was monitored spectrophotometrically. The kinetics of the lytic reaction were characterized with respect to temperature, pH, reactant concentration, and time. Bacteriolysis generally followed saturation kinetics behavior and was apparently a first-order process with respect to the substrate (bacteria). Deviation from a simple Michaelis-Menten relationship, however, was observed at high substrate levels, at which inhibition of lysis was evident. The lytic rate decreased rapidly and sharply through time as the substrate was depleted. Lytic factors in the gut fluid acted as catalysts; rates increased with concentration of gut fluid, but no depletion in lytic capacity through time was observed. The lytic rate increased exponentially for all seasonal samples over the range of environmental temperatures (4 to 31(deg)C) but showed little relationship with pH under conditions likely to be experienced in situ. The lytic rate also varied among bacterial strains and with culture age. 相似文献
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In 1964 and 1965, two silver fish pendants depicting coelacanths and thought to be religious votive from Spanish churches were purchased on the art market in Spain, dated between the 17th and late 19th century, long before the discovery for science of the living coelacanth in 1938. It was speculated that they originated either from the Mediterranean, subtropical Atlantic, or subtropical Pacific (de Sylva 1966, Bruton 1985, Anonymous 1993, Greenwell 1994, Raynold & Mangiacopra 1995). Some authors believe that the silver fish pendants represent a new species of coelacanthiforms (Raynold & Mangiacopra 1995), others claimed the pendants to be only a wishful fantasy of scientists and not coelacanths at all (Thomson 1991). However, new studies revealed that the silver fish artefacts are indeed coelacanths but were in fact produced more recently with the Comorian coelacanth, Latimeria chalumnae, as a model. 相似文献