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71.
Postsynaptic decoding of neural activity: eEF2 as a biochemical sensor coupling miniature synaptic transmission to local protein synthesis 总被引:3,自引:0,他引:3
Activity-dependent regulation of dendritic protein synthesis is critical for enduring changes in synaptic function, but how the unique features of distinct activity patterns are decoded by the dendritic translation machinery remains poorly understood. Here, we identify eukaryotic elongation factor-2 (eEF2), which catalyzes ribosomal translocation during protein synthesis, as a biochemical sensor in dendrites that is specifically and locally tuned to the quality of neurotransmission. We show that intrinsic action potential (AP)-mediated network activity in cultured hippocampal neurons maintains eEF2 in a relatively dephosphorylated (active) state, whereas spontaneous neurotransmitter release (i.e., miniature neurotransmission) strongly promotes the phosphorylation (and inactivation) of eEF2. The regulation of eEF2 phosphorylation is responsive to bidirectional changes in miniature neurotransmission and is controlled locally in dendrites. Finally, direct spatially controlled inhibition of eEF2 phosphorylation induces local translational activation, suggesting that eEF2 is a biochemical sensor that couples miniature synaptic events to local translational suppression in neuronal dendrites. 相似文献
72.
Suppression of gene expression by small interfering RNA (siRNA) has proved to be a gene-specific and cost effective alternative
to other gene suppression technologies. Short hairpin RNAs (shRNAs) generated from the vector-based expression are believed
to be processed into functional siRNAs in vivo, leading to gene silencing. Since an shRNA library carries a large pool of potential siRNAs, such a library makes it possible
to knock down gene expression at the genome wide scale. Although much of research has been focused on generating shRNA libraries
from either individually made gene specific sequences or cDNA libraries, there is no report on constructing randomized shRNA
libraries, which could provide a good alternative to these existing libraries. We have developed a method of constructing
shRNAs from randomized oligonucleotides. Through this method, one can generate a partially or fully randomized shRNA library
for various functional analyses. We validated this procedure by constructing a p53-specific shRNA. Western blot revealed that
the p53-shRNA successfully suppressed expression of the endogenous p53 in MCF-7 cells. We then made a partially randomized
shRNA library. Sequencing of 15 randomly picked cloned confirmed the randomness of the library. Therefore, the library can
be used for various functional assays, such as target validation when a suitable screening or selection method is available. 相似文献
73.
Binh Tran Quang Linh Duong Tuan Chung Le Thi Kim Phuong Pham Tran Nga Bui Thi Thuy Ngoc Nguyen Anh Thuyen Tran Quang Tung Do Dinh Nhung Bui Thi 《Biochemical genetics》2022,60(2):707-719
Biochemical Genetics - The study aimed to evaluate the contribution of the FTO A/T polymorphism (rs9939609) to the prediction of the future type 2 diabetes (T2D). A population-based prospective... 相似文献
74.
Nguyen Anh T. Q. Nguyen Anh M. Nguyen Muu T. Nguyen Hue T. Duong Lim T. Dinh Van M. Nguyen Phuong M. Dultz Stefan Nguyen Minh N. 《Biogeochemistry》2022,160(1):35-47
Biogeochemistry - Phytolith is widely known as a silica structure in numerous silicon (Si) accumulator plants, e.g., rice, and it contains various nutrients and other beneficial elements. When rice... 相似文献
75.
A new species, Hoya hanhiae V. T. Pham et Aver. discovered in central Vietnam is described, illustrated and compared with the related species H. macrophylla Bl. and H. verticillata (Vahl) G. Don. 相似文献
76.
Isolation of New Delhi metallo‐β‐lactamase 1‐producing Vibrio cholerae non‐O1, non‐O139 strain carrying ctxA,st and hly genes in southern Vietnam 下载免费PDF全文
Tai The Diep Nhi Thi Ngoc Nguyen Thi Ngoc Cat Nguyen Huy Khac An Truong Quang Nguyen Vu Hoang Nguyen Thuong Van Nguyen Thu Ngoc Anh Nguyen Hidemasa Izumiya Makoto Ohnishi Tetsu Yamashiro Lan Thi Phuong Nguyen 《Microbiology and immunology》2015,59(5):262-267
Vibrio cholerae non‐O1, non‐O139 (VC_NAG) organisms are universally present in the aquatic environment and regarded as non‐pathogenic bacteria. However, considering that they do occasionally induce gastroenteritis, a study of their virulence and antibiotic resistance genes is important. The presence of enteropathogenic genes, including ctxA, VC_NAG‐specific heat‐stable toxin gene (st), hemolysin (hly), and zona occludens toxin (zot) was determined by PCR in 100 VC_NAG strains isolated in southern Vietnam in 2010–2013 from 94 environmental and six human origins. These 100 VC_NAG strains were also tested phenotypically and genotypically for the presence of the New Delhi metallo‐β‐lactamase (NDM‐1). Of the 100 VC_NAG strains tested, six were positive for ctxA; five from the environment and one of human origin. The st gene was detected in 17 isolates, 15 and two of which were of environmental and human origins, respectively. Gene hly was detected in 19 VC_NAG strains examined, two of which were isolated from humans and 17 from environments. The zot gene was not detected in any of the strains tested. Three VC_NAG strains of environmental origin were confirmed to produce NDM‐1 and the blaNDM‐1 gene was detected in those strains by PCR. Of note, one of the three NDM‐1‐producing VC_NAG strains was confirmed to carry ctxA, st and hly genes concurrently. This is the first report of isolation of NDM‐1‐producing VC_NAG strains in Vietnam. 相似文献
77.
Whole genome analysis of Vietnamese G2P[4] rotavirus strains possessing the NSP2 gene sharing an ancestral sequence with Chinese sheep and goat rotavirus strains 下载免费PDF全文
Loan Phuong Do Toyoko Nakagomi Punita Gauchan Miho Kaneko Chantal Agbemabiese Anh Duc Dang Osamu Nakagomi 《Microbiology and immunology》2015,59(10):605-613
Because imminent introduction into Vietnam of a vaccine against Rotavirus A is anticipated, baseline information on the whole genome of representative strains is needed to understand changes in circulating strains that may occur after vaccine introduction. In this study, the whole genomes of two G2P[4] strains detected in Nha Trang, Vietnam in 2008 were sequenced, this being the last period during which virtually no rotavirus vaccine was used in this country. The two strains were found to be > 99.9% identical in sequence and had a typical DS‐1 like G2‐P[4]‐I2‐R2‐C2‐M2‐A2‐N2‐T2‐E2‐H2 genotype constellation. Analysis of the Vietnamese strains with > 184 G2P[4] strains retrieved from GenBank/EMBL/DDBJ DNA databases placed the Vietnamese strains in one of the lineages commonly found among contemporary strains, with the exception of the NSP2 and NSP4 genes. The NSP2 genes were found to belong to a previously undescribed lineage that diverged from Chinese sheep and goat rotavirus strains, including a Chinese rotavirus vaccine strain LLR with 95% nucleotide identity; the time of their most recent common ancestor was 1975. The NSP4 genes were found to belong, together with Thai and USA strains, to an emergent lineage (VIII), adding further diversity to ever diversifying NSP4 lineages. Thus, there is a need to enhance surveillance of locally‐circulating strains from both children and animals at the whole genome level to address the effect of rotavirus vaccines on changing strain distribution. 相似文献
78.
79.
Guanmei Wen Cheng Zhang Qishan Chen Le Anh Luong Arif Mustafa Shu Ye Qingzhong Xiao 《The Journal of biological chemistry》2015,290(31):19158-19172
Matrix metalloproteinase-8 (MMP8) has been shown to influence various cellular functions. As monocytes and macrophages (Mφ) express MMP8, we investigated if MMP8 played a role in macrophage differentiation and polarization. MMP8 expression was significantly increased during monocyte differentiation into Mφ. Monocyte-derived Mφ from MMP8-deficient mice expressed higher levels of M1-Mφ markers but lower levels of M2-Mφ markers than monocyte-derived Mφ from wild-type mice. Although Mφ from either MMP8-deficient or wild-type mice were inducible by interferon-γ into M1-Mφ, only wild-type Mφ but not MMP8-deficient Mφ could be induced into M2-Mφ by interleukin-4. However, MMP8-deficient Mφ exposed to conditioned culture media of wild-type Mφ developed a M2-Mφ phenotype. Compared with conditioned culture media of wild-type Mφ, conditioned culture media of MMP8-deficient Mφ contained a lower concentration of active transforming growth factor-β (TGF-β), an M2-Mφ inducer. Moreover, evidence also showed that the degradation of the TGF-β sequester, fibromodulin, was modulated by MMP8. The data indicate a previously unknown role of MMP8 in M2-Mφ polarization by cleaving fibromodulin and therefore increasing the bioavailability of the M2-Mφ inducer TGF-β. 相似文献
80.