排序方式: 共有136条查询结果,搜索用时 187 毫秒
131.
Fan Zheng Tianpeng Li Dong-yan Jin Viktoriya Syrovatkina Kathleen Scheffler Phong T. Tran Chuanhai Fu 《Molecular biology of the cell》2014,25(18):2750-2760
Accurate chromosome segregation requires timely bipolar spindle formation during mitosis. The transforming acidic coiled-coil (TACC) family proteins and the ch-TOG family proteins are key players in bipolar spindle formation. They form a complex to stabilize spindle microtubules, mainly dependent on their localization to the centrosome (the spindle pole body [SPB] in yeast). The molecular mechanism underlying the targeting of the TACC–ch-TOG complex to the centrosome remains unclear. Here we show that the fission yeast Schizosaccharomyces pombe TACC orthologue alp7p is recruited to the SPB by csi1p. The csi1p-interacting region lies within the conserved TACC domain of alp7p, and the carboxyl-terminal domain of csi1p is responsible for interacting with alp7p. Compromised interaction between csi1p and alp7p impairs the localization of alp7p to the SPB during mitosis, thus delaying bipolar spindle formation and leading to anaphase B lagging chromosomes. Hence our study establishes that csi1p serves as a linking molecule tethering spindle-stabilizing factors to the SPB for promoting bipolar spindle assembly. 相似文献
132.
Van Tang Tai Rene Eldon R. Binh Tran Ngoc Behera Shishir Kumar Phong Nguyen Tan 《Wetlands Ecology and Management》2020,28(1):163-176
Wetlands Ecology and Management - This study aims to investigate the diversity of mangrove species and their soil erosion mitigation performance in Hung Hoa Mangrove Forest, Vinh City, Vietnam.... 相似文献
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134.
Anthony R. Gangloff Jason Brown Ron de Jong Douglas R. Dougan Charles E. Grimshaw Mark Hixon Andy Jennings Ruhi Kamran Andre Kiryanov Shawn O’Connell Ewan Taylor Phong Vu 《Bioorganic & medicinal chemistry letters》2013,23(16):4501-4505
Structure based drug design of a series of novel 1,4-benzoxazin-3-one derived PARP-1 inhibitors are described. The synthesis, enzymatic & cellular activities and pharmacodynamic effects are described. Optimized analogs demonstrated inhibition of poly-ADP-ribosylation in SW620 tumor bearing nude mice through 24 h following a single dose. 相似文献
135.
Phong Huynh Xuan Klanrit Preekamol Dung Ngo Thi Phuong Yamada Mamoru Thanonkeo Pornthap 《Annals of microbiology》2019,69(7):765-776
The purpose of this study was to isolate, identify, and characterize the thermotolerant yeasts for use in high-temperature ethanol fermentation. Thermotolerant yeasts were isolated and screened from soil samples collected from the Mekong Delta, Vietnam, using the enrichment method. Classification and identification of the selected thermotolerant yeasts were performed using matrix-assisted laser desorption ionization/time-of-fight mass spectrometry (MALDI-TOF/MS) and nucleotide sequencing of the D1/D2 domain of the 26S rDNA and the internal transcribed spacer (ITS) 1 and 2 regions. The ethanol production by the selected thermotolerant yeast was carried out using pineapple waste hydrolysate (PWH) as feedstock. A total of 174 yeast isolates were obtained from 80 soil samples collected from 13 provinces in the Mekong Delta, Vietnam. Using MALDI-TOF/MS and nucleotide sequencing of the D1/D2 domain and the ITS 1 and 2 regions, six different yeast species were identified, including Meyerozyma caribbica, Saccharomyces cerevisiae, Candida tropicalis, Torulaspora globosa, Pichia manshurica, and Pichia kudriavzevii. Among the isolated thermotolerant yeasts, P. kudriavzevii CM4.2 displayed great potential for high-temperature ethanol fermentation. The maximum ethanol concentration (36.91 g/L) and volumetric ethanol productivity (4.10 g/L h) produced at 45 °C by P. kudriavzevii CM4.2 were achieved using PWH containing 103.08 g/L of total sugars as a feedstock. These findings clearly demonstrate that the newly isolated thermotolerant yeast P. kudriavzevii CM4.2 has a high potential for second-generation bioethanol production at high temperature. 相似文献
136.
Phong T. Le Richard F. Mortensen 《In vitro cellular & developmental biology. Plant》1984,20(6):505-511
Summary A methodology for obtaining reproducible in vitro induction of the synthesis of the acute phase reactant serum amyloid P-component
(SAP) by purified mouse hepatocytes was established. Optimal hepatocyte culture conditions for the induction and synthesis
of SAP required certain hormones, a substratum for cell attachment, and activated macrophages. Leibowitz L15 medium had to
be supplemented with dexamethasone, indomethacin, insulin, glucose, and fetal bovine serum. Purified mouse IL 1 could substitute
for activated macrophages in the induction of SAP. Hepatocytes were allowed to adhere to a collagen matrix to enhance both
cell viability and SAP synthesis induced by IL 1. Elicited macrophages cultured with hepatocytes were capable of augmenting
SAP synthesis in the presence of IL 1.
This study was supported by Grant CA-30015 from the National Institutes of Health, Bethesda, MD. 相似文献