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961.
962.
Given the rarity of hybridization in seabirds, which presumably relates to their very high philopatry, the degree of breeding‐site vagrancy should correspond with the incidence of mixed‐species pairing, although not necessarily with the production of hybrids if there are behavioural or genetic barriers to successful reproduction. Using molecular methods, we verified that two of the three chicks hatched by a vagrant male White‐capped Albatross Thalassarche steadi paired with a female Black‐browed Albatross Thalassarche melanophris at South Georgia were genuine hybrids (these chicks died before fledging, but a third chick – the result of an extra‐pair copulation – fledged successfully). In a wider review, we could find only five known or suspected mixed‐species pairs, and three different hybrids in albatrosses, mostly between closely related species. This appears to reflect behavioural barriers to hybridization in sympatric species and the low incidence of breeding‐site vagrancy (which mainly involves single individuals that invariably associate with the most phenotypically similar local taxon). Breeding‐site vagrancy is most frequent in the ‘shy‐albatross’ complex, which could explain why genetic divergence occurred more recently in this group than in other Thalassarche, and hence exploratory behaviour appears to be more important than numerical abundance or breeding distribution in driving colonization as well as hybridization processes in albatrosses. 相似文献
963.
At‐vessel mortality of skates (Rajidae) taken in coastal fisheries and evidence of longer‐term survival
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J. R. Ellis G. J. Burt G. Grilli S. R. McCully Phillips T. L. Catchpole D. L. Maxwell 《Journal of fish biology》2018,92(6):1702-1719
Data on the vigour and at‐vessel mortality (AVM) of 6798 skates (comprising Raja clavata n = 6295; R. brachyura n = 208; R. undulata n = 185, R. montagui n = 98 and R. microocellata n = 12) captured by commercial fishing vessels in the inshore waters of the southern North Sea and English Channel were recorded. AVM in longline fisheries averaged 0·44% across five vessels (0–1·47%), although skates were usually unhooked manually and did not usually pass through a bait‐stripper. AVM in otter trawls averaged 0·76% (0–2·35%), from four vessels fishing with tow durations of <1·5 h (southern North Sea) or 1–4 h (English Channel). No AVM was noted for skates taken as a by‐catch in drift trammel nets (soak times <4 h). Anchored tangle nets resulted in an overall AVM of 2·0–2·7%, but increased from 1·47% (13–28 h soak time) to 6·16% (42–53 h soak time). There were significant differences in the vigour of skates between gears, with R. clavata caught by longline and tangle nets in better condition than those captured by otter trawl or drift trammel net. Similarly, R. undulata caught by tangle net were in better condition than those caught by otter trawl. The vigour of R. undulata was also found to be higher than other skate species for both trawl and tangle net. In total, 5283 skates were tagged with Petersen discs and released, with recapture rates for the various combinations of vessel and gear ranging up to 24·8% for R. clavata. Whilst confirming a degree of post‐release survival, quantitative estimates of post‐release mortality for skates remain unknown. 相似文献
964.
965.
Life span and reproductive cost explain interspecific variation in the optimal onset of reproduction
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Emeline Mourocq Pierre Bize Sandra Bouwhuis Russell Bradley Anne Charmantier Carlos de la Cruz Szymon M. Drobniak Richard H. M. Espie Márton Herényi Hermann Hötker Oliver Krüger John Marzluff Anders P. Møller Shinichi Nakagawa Richard A. Phillips Andrew N. Radford Alexandre Roulin János Török Juliana Valencia Martijn van de Pol Ian G. Warkentin Isabel S. Winney Andrew G. Wood Michael Griesser 《Evolution; international journal of organic evolution》2016,70(2):296-313
Fitness can be profoundly influenced by the age at first reproduction (AFR), but to date the AFR–fitness relationship only has been investigated intraspecifically. Here, we investigated the relationship between AFR and average lifetime reproductive success (LRS) across 34 bird species. We assessed differences in the deviation of the Optimal AFR (i.e., the species‐specific AFR associated with the highest LRS) from the age at sexual maturity, considering potential effects of life history as well as social and ecological factors. Most individuals adopted the species‐specific Optimal AFR and both the mean and Optimal AFR of species correlated positively with life span. Interspecific deviations of the Optimal AFR were associated with indices reflecting a change in LRS or survival as a function of AFR: a delayed AFR was beneficial in species where early AFR was associated with a decrease in subsequent survival or reproductive output. Overall, our results suggest that a delayed onset of reproduction beyond maturity is an optimal strategy explained by a long life span and costs of early reproduction. By providing the first empirical confirmations of key predictions of life‐history theory across species, this study contributes to a better understanding of life‐history evolution. 相似文献
966.
Constance M. John Nancy J. Phillips Richard Din Mingfeng Liu Einar Rosenqvist E. Arne H?iby Daniel C. Stein Gary A. Jarvis 《The Journal of biological chemistry》2016,291(7):3224-3238
The degree of phosphorylation and phosphoethanolaminylation of lipid A on neisserial lipooligosaccharide (LOS), a major cell-surface antigen, can be correlated with inflammatory potential and the ability to induce immune tolerance in vitro. On the oligosaccharide of the LOS, the presence of phosphoethanolamine and sialic acid substituents can be correlated with in vitro serum resistance. In this study, we analyzed the structure of the LOS from 40 invasive isolates and 25 isolates from carriers of Neisseria meningitidis without disease. Invasive strains were classified as groups 1–3 that caused meningitis, septicemia without meningitis, and septicemia with meningitis, respectively. Intact LOS was analyzed by high resolution matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Prominent peaks for lipid A fragment ions with three phosphates and one phosphoethanolamine were detected in all LOS analyzed. LOS from groups 2 and 3 had less abundant ions for highly phosphorylated lipid A forms and induced less TNF-α in THP-1 monocytic cells compared with LOS from group 1. Lipid A from all invasive strains was hexaacylated, whereas lipid A of 6/25 carrier strains was pentaacylated. There were fewer O-acetyl groups and more phosphoethanolamine and sialic acid substitutions on the oligosaccharide from invasive compared with carrier isolates. Bioinformatic and genomic analysis of LOS biosynthetic genes indicated significant skewing to specific alleles, dependent on the disease outcome. Our results suggest that variable LOS structures have multifaceted effects on homeostatic innate immune responses that have critical impact on the pathophysiology of meningococcal infections. 相似文献
967.
Lucas A. Cernusak Margaret M. Barbour Stefan K. Arndt Alexander W. Cheesman Nathan B. English Taylor S. Feild Brent R. Helliker Meisha M. Holloway‐Phillips Joseph A.M. Holtum Ansgar Kahmen Francesca A. McInerney Niels C. Munksgaard Kevin A. Simonin Xin Song Hilary Stuart‐Williams Jason B. West Graham D. Farquhar 《Plant, cell & environment》2016,39(5):1087-1102
Leaf water contains naturally occurring stable isotopes of oxygen and hydrogen in abundances that vary spatially and temporally. When sufficiently understood, these can be harnessed for a wide range of applications. Here, we review the current state of knowledge of stable isotope enrichment of leaf water, and its relevance for isotopic signals incorporated into plant organic matter and atmospheric gases. Models describing evaporative enrichment of leaf water have become increasingly complex over time, reflecting enhanced spatial and temporal resolution. We recommend that practitioners choose a model with a level of complexity suited to their application, and provide guidance. At the same time, there exists some lingering uncertainty about the biophysical processes relevant to patterns of isotopic enrichment in leaf water. An important goal for future research is to link observed variations in isotopic composition to specific anatomical and physiological features of leaves that reflect differences in hydraulic design. New measurement techniques are developing rapidly, enabling determinations of both transpired and leaf water δ18O and δ2H to be made more easily and at higher temporal resolution than previously possible. We expect these technological advances to spur new developments in our understanding of patterns of stable isotope fractionation in leaf water. 相似文献
968.
Transmembrane domain of myelin protein zero can form dimers: possible implications for myelin construction 总被引:1,自引:0,他引:1
Plotkowski ML Kim S Phillips ML Partridge AW Deber CM Bowie JU 《Biochemistry》2007,46(43):12164-12173
Myelin protein zero (MPZ) is the major integral membrane protein of peripheral nerve myelin in higher vertebrates, mediating homoadhesion of the multiple, spiraling wraps of the myelin sheath. Previous studies have shown that full-length MPZ can form dimers and tetramers, and biochemical studies on the extracellular domain (ECD) indicate that it can form a tetramer, albeit very weakly. On the basis of cross-linking studies and equilibrium sedimentation of a transmembrane (TM) domain peptide (MPZ-TM), we find that the MPZ-TM can form homodimers. We further characterized the dimer by measuring the effects of alanine and leucine substitutions on the ability of the TM to dimerize in Escherichia coli membranes. Our results indicate that the primary packing interface for the MPZ TM homodimer is a glycine zipper (GxxxGxxxG) motif. We also find that the G134R mutation, which lies within the glycine zipper packing interface and causes Charcot-Marie-Tooth disease type 1B, severely inhibits dimerization, suggesting that dimerization of the TM domain may be important for the normal functioning of MPZ. By combining our new results with prior work, we suggest a new model for an MPZ lattice that may form during the construction of myelin. 相似文献
969.
Daly NL Chen YK Rosengren KJ Marx UC Phillips ML Waring AJ Wang W Lehrer RI Craik DJ 《Biochemistry》2007,46(35):9920-9928
Retrocyclins are circular mini-defensins with significant potential as agents against human immunodeficiency virus, influenza A, and herpes simplex virus. Retrocyclins bind carbohydrate-containing surface molecules such as gp120 and CD4 with high affinity (Kd, 10-100 nM), promoting their localization on cell membranes. The structural features important for activity have yet to be fully elucidated, but here, we have determined the first three-dimensional structure of a retrocyclin, namely, one of the most potent forms, retrocyclin-2. In the presence of SDS micelles, a well-defined beta-hairpin braced by three disulfide bonds that defines the cystine ladder motif is present. By contrast, a well-defined structure could not be determined in aqueous solution, suggesting that the presence of SDS micelles stabilizes the extended conformation of retrocyclin-2. Translational diffusion measurements indicate that retrocyclin-2 interacts with the SDS micelles, and such a membrane-like interaction may be an important feature in the mechanism of action of these antimicrobial peptides. Analytical ultracentrifugation and the NMR data indicated that retrocyclin-2 self-associates to form a trimer in a concentration-dependent manner. The ability to self-associate may contribute to the high-affinity binding of retrocyclins for glycoproteins by increasing the valency and enhancing the ability of retrocyclins to cross-link cell surface glycoproteins. 相似文献
970.
Since the discovery of the ancient eukaryotic process of RNA-mediated gene silencing, the reverse-genetics technique RNA interference (RNAi) has increasingly been used to examine gene function in vertebrate and invertebrate systems. In this study, we report on the use of RNAi, adapted from studies on animal model systems, to manipulate gene expression in a symbiotic marine cnidarian. We describe gene knockdown of actin and of acasp--a cysteine protease, or caspase--in the symbiotic sea anemone Aiptasia pallida. Knockdown was assessed qualitatively with in situ hybridizations for both genes. Quantitative PCR and caspase activity assays were used as a quantitative measure of knockdown for acasp. 相似文献