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81.
Philippe Clerc 《Nordic Journal of Botany》1987,7(4):479-495
Systematics and taxonomy of Usnea cornuta, U. flammea and U. fragilescens are discussed. The morphology, chemistry and distribution of these species in Europe are described and a first attempt is made to provide a key for the erect-bushy to subpendant and sorediate species in Scandinavia. Compounds reported in this aggregate include lobaric acid (new to the genus). U. flammea is new for Scandinavia. U. constrictula, U. inflate, U. intexla and U. subpectinata are reduced to synonymy with U. cornuta, U. dalmatica and U. rupestris to synonymy with U. flammea, U. glaucescens to synonymy with U. hirta and the new combination U. fragilescens var. mollis is proposed. 相似文献
82.
Adult mouse liver nuclei and their subfractions corresponding to heterochromatin, nucleoli, membranes, and euchromatin were studied for DNA-polymerase activity. The intact nuclei and the two heavy nuclear fractions contained rather low activity while the two light fractions (membranes and euchromatin) had no activity at all. In the two heavy fractions, the activity was stimulated by β-mercaptoethanol and depressed by p-hydroxymercuribenzoate and by omission of one or more nucleotides. A nuclease activity, detected in the intact nuclei, may also be present in the nuclear subfractions. DNA-polymerase activity in the heavy fractions of mouse liver nuclei is discussed in relation to other published results. 相似文献
83.
The efficiency of ethyleneglycol-bis (β-amino-ethyl ether) N,N′-tetra-acetic acid (EGTA) in removing possible contamination from myelin was tested. Myelin fractions were isolated in the presence or absence of EGTA. An axolemma-enriched fraction was also prepared. Gel electrophoresis showed no important alteration of the protein pattern of myelin treated with EGTA. Only a minor band of about 41,000 daltons was selectively removed when EGTA was used during the two density gradient and differential centrifugation steps. EGTA, when used in the final washes, did not remove this band. It was absent from axolemma-enriched fractions. Different hypotheses are considered to explain these findings. 相似文献
84.
Yann Thierry Le Guen Tony Le Gall Patrick Midoux Philippe Gugan Serge Braun Tristan Montier 《The journal of gene medicine》2020,22(2)
Hydrodynamic limb vein injection is an in vivo locoregional gene delivery method. It consists of administrating a large volume of solution containing nucleic acid constructs in a limb with both blood inflow and outflow temporarily blocked using a tourniquet. The fast, high pressure delivery allows the musculature of the whole limb to be reached. The skeletal muscle is a tissue of choice for a variety of gene transfer applications, including gene therapy for Duchenne muscular dystrophy or other myopathies, as well as for the production of antibodies or other proteins with broad therapeutic effects. Hydrodynamic limb vein delivery has been evaluated with success in a large range of animal models. It has also proven to be safe and well‐tolerated in muscular dystrophy patients, thus supporting its translation to the clinic. However, some possible limitations may occur at different steps of the delivery process. Here, we have highlighted the interests, bottlenecks and potential improvements that could further optimize non‐viral gene transfer following hydrodynamic limb vein injection. 相似文献
85.
Salom Fabri‐Ruiz Bruno Danis Nicolas Navarro Philippe Koubbi Rmi Laffont Thomas Saucde 《Global Change Biology》2020,26(4):2161-2180
The Southern Ocean (SO) is among the regions on Earth that are undergoing regionally the fastest environmental changes. The unique ecological features of its marine life make it particularly vulnerable to the multiple effects of climate change. A network of Marine Protected Areas (MPAs) has started to be implemented in the SO to protect marine ecosystems. However, considering future predictions of the Intergovernmental Panel on Climate Change (IPCC), the relevance of current, static, MPAs may be questioned under future scenarios. In this context, the ecoregionalization approach can prove promising in identifying well‐delimited regions of common species composition and environmental settings. These so‐called ecoregions are expected to show similar biotic responses to environmental changes and can be used to define priority areas for the designation of new MPAs and the update of their current delimitation. In the present work, a benthic ecoregionalization of the entire SO is proposed for the first time based on abiotic environmental parameters and the distribution of echinoid fauna, a diversified and common member of Antarctic benthic ecosystems. A novel two‐step approach was developed combining species distribution modeling with Random Forest and Gaussian Mixture modeling from species probabilities to define current ecoregions and predict future ecoregions under IPCC scenarios RCP 4.5 and 8.5. The ecological representativity of current and proposed MPAs of the SO is discussed with regard to the modeled benthic ecoregions. In all, 12 benthic ecoregions were determined under present conditions, they are representative of major biogeographic patterns already described. Our results show that the most dramatic changes can be expected along the Antarctic Peninsula, in East Antarctica and the sub‐Antarctic islands under both IPCC scenarios. Our results advocate for a dynamic definition of MPAs, they also argue for improving the representativity of Antarctic ecoregions in proposed MPAs and support current proposals of Conservation of Antarctic Marine Living Resources for the creation of Antarctic MPAs. 相似文献
86.
Jacob A. Toth George M. Stack Ali R. Cala Craig H. Carlson Rebecca L. Wilk Jamie L. Crawford Donald R. Viands Glenn Philippe Christine D. Smart Jocelyn K. C. Rose Lawrence B. Smart 《Global Change Biology Bioenergy》2020,12(3):213-222
Hemp (Cannabis sativa L.) is an emerging dioecious crop grown primarily for grain, fiber, and cannabinoids. There is good evidence for medicinal benefits of the most abundant cannabinoid in hemp, cannabidiol (CBD). For CBD production, female plants producing CBD but not tetrahydrocannabinol (THC) are desired. We developed and validated high‐throughput PACE (PCR Allele Competitive Extension) assays for C. sativa plant sex and cannabinoid chemotype. The sex assay was validated across a wide range of germplasm and resolved male plants from female and monoecious plants. The cannabinoid chemotype assay revealed segregation in hemp populations, and resolved plants producing predominantly THC, predominantly CBD, and roughly equal amounts of THC and CBD. Cultivar populations that were thought to be stabilized for CBD production were found to be segregating phenotypically and genotypically. Many plants predominantly producing CBD accumulated more than the current US legal limit of 0.3% THC by dry weight. These assays and data provide potentially useful tools for breeding and early selection of hemp. 相似文献
87.
Systematic Parasitology - The proteocephalid genus Pseudoendorchis (Cestoda: Onchoproteocephalidea) has recently been proposed to accommodate seven species/species-level lineages of tapeworm... 相似文献
88.
Hunter Elena Percival Benita Ahmad Zeeshan Chang Ming-Wei Hunt John A. Tasker Séverine De Risio Luisa Wilson Philippe B. 《Molecular and cellular biochemistry》2021,476(11):4133-4137
Molecular and Cellular Biochemistry - Chronic kidney disease (CKD) is a renal dysfunction that can lead to high rates of mortality and morbidity, particularly when coupled with late diagnosis. CKD... 相似文献
89.
90.
Schrijvers Dieuwertje L. Loubet Philippe Sonnemann Guido 《The International Journal of Life Cycle Assessment》2021,26(6):1223-1235
The International Journal of Life Cycle Assessment - How to apply allocation in an life cycle assessment (LCA) is a long-running and controversial debate. Consensus seems to exist on the fact that... 相似文献