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61.
Leopold J. Anghileri Philippe Maincent Alfredo Cordova Martinez Pierre Thouvenot 《Biological trace element research》1994,46(1-2):103-111
The effects of the interaction between low molecular weight iron complexes (citrate, lactate, and ATP complexes) with ATP and proteins, on the modification of Ehrlich carcinoma cell calcium homeostasis have been studied. In that modification the ferric-ATP complex shows much higher activity than the others. Sodium ATP, by iron translocation from citrate and lactate, increases their activity. This phenomenon implicates ATP as a mediator on the cellular activity of the complexes. Proteins, particularly ferritin, appear to moderately reduce their activity, whereas glutathione and ascorbic acid, acting as lipid peroxidation-inhibitors, show only a slight reduction of the iron complex’s effects on cellular calcium uptake. 相似文献
62.
The effect of exogenous abscisic acid, provided to somatic embryos during the maturation step, on endogenous abscisic acid and its main conjugated form (abscisic acid glucose ester), germination and conversion frequencies is presented in this paper. Abscisic acid measurements were obtained after a methanolic extraction, a fractionation through high performance liquid chromatography, quantitation with an immunoassay and identification of the quantitated compound using gas chromatography-mass spectrometry. Results show that endogenous abscisic acid and abscisic acid glucose ester levels are clearly correlated with the exogenous abscisic acid concentration provided to the embryos. Maturation was clearly enhanced by exogenous abscisic acid, but no correlation was found between abscisic acid concentration and germination frequency. Conversely, development of the aerial part of the germinated somatic embryos was dependent upon the abscisic acid concentration in the culture medium and results suggest that this dependence could be related to the endogenous abscisic acid content. 相似文献
63.
Changes in carbon storage in temperate humic loamy soils after forest clearing and continuous corn cropping in France 总被引:3,自引:0,他引:3
Soil samples from forest and agricultural sites in three areas of southwest France were collected to determine the effect
of forest conversion to continuous intensive corn cropping with no organic matter management on soil organic carbon (C) content.
Soils were humic loamy soils and site characteristics that may affect soil C were as uniform as possible (slope, elevation,
texture, soil type, vegetation).
Three areas were selected, with adjacent sites of various ages of cultivation (3 to 35 yr), and paired control forest sites.
The ploughed horizon (0-Dt cm) and the Dt-50 cm layer were collected at each agricultural site. In forest sites, each 10 cm
layer was collected systematically down to 1 meter depth. Carbon concentrations were converted to total content to a given
depth as the product of concentration, depth of sample and bulk density, and expressed in units of kg m-2. For each site and each sampled layer, the mineral mass of soil was calculated, in order to base comparisons on the same
soil mass rather than the same depth.
The pattern of C accumulation in forest soils showed an exponential decrease with depth. Results suggested that soil organic
carbon declined rapidly during the first years of cultivation, and at a slower rate thereafter. This pattern of decrease can
be fitted by a bi-exponential model assuming that initial soil organic carbon can be separated into two parts, a very labile
pool reduced during the first rapid decline and more refractory fractions oxidizing at a slower rate. Sampling to shallow
depths (0-Dt cm) resulted in over-estimation of the rate of carbon release in proportion to the initial amount of C, and in
under-estimation of the total loss of C with age. The results for the 0–50 cm horizon indicated that losses of total carbon
average about 50% in these soils, ranging in initial carbon content from 19 to 32.5 kg m-2. Carbon release to the atmosphere averaged 0.8 kg m-2 yr-1 to 50 cm depth during the first 10 years of cultivation. The results demonstrate that temperate soils may also be an important
source of atmospheric carbon, when they are initially high in carbon content and then cultivated intensively with no organic
matter management. 相似文献
64.
Alain Delbarre Philippe Muller Viviane Imhoff Jean-Louis Morgat Hélène Barbier-Brygoo 《Planta》1994,195(2):159-167
Uptake and metabolism of exogenous naphthalene-1-acetic acid (NAA) and indole-3-acetic acid (IAA) have been studied in tobacco (Nicotiana tabacum L. cv. Xanthi) mesophyll protoplasts. Both auxins entered protoplasts by diffusion under the action of the transmembrane pH gradient without any detectable participation of an influx carrier. Molecules were accumulated by an anion-trapping mechanism and most of them were metabolized within hours, essentially as glucose-ester and amino-acid conjugates. Protoplasts were equipped with a functional auxin-efflux carrier as evidenced by the inhibitory effect of naphthylphtalamic acid on IAA efflux. Basically, similar mechanisms of NAA and IAA uptake occurred in protoplasts. However, the two auxins differed in their levels of accumulation, due to different membrane-transport characteristics, and the nature of the metabolites produced. This shows the need to estimate the accumulation and the metabolism of auxins when analyzing their effects in a given cell system. The internal auxin concentration could be modulated by changing the transmembrane pH gradient, giving an interesting perspective for discriminating between the effects of intra- and extracellular auxin on physiological processes.Abbreviations BA
benzoic acid
- Ci/Ce
accumulation ratio of auxin
- IAAasp
N-[3-indolylacetyl]-dl-aspartic acid
- NAA
naphthalene-1-acetic acid
- NAAasp
N-[1-naphthylacetyl]-l-aspartic acid
- NPA
N-1-naphthylphthalamic acid
The authors thank Dr. M. Caboche (I.N.R.A, Versailles, France) for his generous gifts of some amide derivatives of 1-NAA, Mr. P. Varennes and Dr. B. Das (I.C.S.N., C.N.R.S., Gif-sur-Yvette, France) for recording and interpreting the mass spectra of NAA glucose ester, and Prof. P. Manigault (Institut des Sciences Végétales, Gif-sur-Yvette) for microscopy measurements of protoplast dimensions. This work was supported by funds from the C.N.R.S, I.N.R.A, and E.E.C. 相似文献
65.
Mainul Hassan Philippe J. Blanc Alain Pareilleux Gérard Goma 《Biotechnology letters》1994,16(8):819-824
Summary A search was carried out for mutants, defective in the conversion of stearic acid to oleic acid in effort to improve the quality of lipids produced by Cryptococcus curvatus ATCC, 20509. Mutants were selected as unsaturated fatty acid (Ufa) auxotrophs. After treatment of parent organism with Ethyl methanesulfonate (EMS), 11 oleate-requiring auxotrophs were isolated. Only 3 of them were real unsaturated fatty acid (Ufa) mutants, while the other 8 were designated as fatty acid synthetase (Fas) mutants. The amount of saturated fatty acid (SFA) was about 65.2 % in the lipids extracted from an Ufa mutant named UfaM3 and it was significantly higher than that of the wild-type (WT) (46.6 %) and similar to that of cocoa butter (60.4 %). 相似文献
66.
Analysis of phytochrome- and ABA-deficient mutants suggests that ABA degradation is controlled by light in Nicotiana plumbaginifolia 总被引:6,自引:2,他引:4
Yvan Kraepiel Philippe Rousselin Bruno Sotta Lucien Kerhoas Jacques Einhorn Michel Caboche Emile Miginiac 《The Plant journal : for cell and molecular biology》1994,6(5):665-672
The phytochrome chromophore-deficient mutant, pew1, of Nicotiana plumbaginifolia exhibited decreased germination and slower dehydration of detached leaves during water stress as compared with the wild-type. These physiological processes are controlled by abscisic acid (ABA) and we examined, therefore, whether phytochrome plays a specific role in the regulation of ABA metabolism using the pew1 mutant. The ABA contents of mature seeds and young leaves were analysed and in both cases mutant material was found to contain higher amounts of ABA as compared with the wild-type. This indicates that the phytochrome activation can lead to a decrease of the ABA level in the wild-type plant. The role of phytochromes was investigated in greater detail using the ABA-deficient mutant aba1 of N. plumbaginifolia exhibiting an early and synchronous germination. This mutant accumulates at very high levels a metabolite derived from a precursor (ABA-aldehyde) in the ABA biosynthetic pathway. The first biochemical characterization of this molecule, which corresponds to the glucose-conjugated ABA-alcohol (ABA-AG) is described. A pew1-aba1 double mutant exhibiting both an etiolated growth and early germination was also obtained. The comparable accumulation of ABA-AG in the pew1-aba1 double mutant as compared with the aba1 mutant allowed the proposition that, in a wild-type plant, the phytochrome-mediated light signal enhances ABA degradation rather than inhibits its biosynthesis. 相似文献
67.
The soil fauna: the other last biotic frontier 总被引:4,自引:0,他引:4
Different approaches to biodiversity yield global totals as small as 3 million or as large as 80 million species. Erwin's calculation and estimation leads to an estimate of ca 30 million species and relies on four assumptions of which one concerns the ratio between the number of canopy insects and those found elsewhere, especially in the soil. A short survey of the microarthropods living in coastal sand dunes and collected with a new flotation method yielded amazing results. In spite of the severity of the habitat (low organic matter content and extreme dryness), the density of microarthropods varied between 175 000 and 1 400 000 individuals per square metre, i.e., densities 3 to 10 times higher than densities usually observed in any other type of soil. A total of 31 species was recorded, most undescribed and smaller than 200 m. The consequences of these findings on the estimation of the number of species are discussed. It is suggested that the soil, including the deepest horizons and the rhizosphere, might constitute a huge reservoir for biodiversity. 相似文献
68.
Nathalie Knittel Véronique Gruber Günther Hahne Philippe Lénée 《Plant cell reports》1994,14(2-3):81-86
Summary A reliable protocol for the transformation of cultivated sunflower (Helianthus annuus L.) has been established, based on microprojectile bombardment of half shoot apices in combination with Agrobacterium tumefaciens coculture. Transgenic shoots have been obtained from 5 inbred lines, although transformation efficiencies varied with the genotype. Plants expressing the transgenes could be recovered from up to 7% of the explants. A minority of plants was shown to be chimaeric for expression of ß-glucuronidase activity while most appeared to be uniformly transformed. Genetic segregation was 31 for both ß-glucuronidase and neomycine phospho transferase in some plants, indicating that the respective mother plants were uniformly transformed. Integration of the foreign genes was also shown by Southern analysis.Abbreviations BAP
benzyl amino purine
- EDTA
ethylene diamine tetraacetic acid
- GUS
ß-glucuronidase
- npt II
neomycine phospho-transferase II 相似文献
69.
70.