全文获取类型
收费全文 | 9819篇 |
免费 | 800篇 |
国内免费 | 9篇 |
专业分类
10628篇 |
出版年
2023年 | 42篇 |
2022年 | 81篇 |
2021年 | 171篇 |
2020年 | 112篇 |
2019年 | 110篇 |
2018年 | 160篇 |
2017年 | 131篇 |
2016年 | 270篇 |
2015年 | 413篇 |
2014年 | 502篇 |
2013年 | 628篇 |
2012年 | 776篇 |
2011年 | 763篇 |
2010年 | 450篇 |
2009年 | 441篇 |
2008年 | 598篇 |
2007年 | 653篇 |
2006年 | 615篇 |
2005年 | 529篇 |
2004年 | 539篇 |
2003年 | 515篇 |
2002年 | 514篇 |
2001年 | 116篇 |
2000年 | 83篇 |
1999年 | 118篇 |
1998年 | 147篇 |
1997年 | 104篇 |
1996年 | 95篇 |
1995年 | 104篇 |
1994年 | 90篇 |
1993年 | 82篇 |
1992年 | 92篇 |
1991年 | 43篇 |
1990年 | 49篇 |
1989年 | 35篇 |
1988年 | 38篇 |
1987年 | 24篇 |
1986年 | 33篇 |
1985年 | 33篇 |
1984年 | 33篇 |
1983年 | 27篇 |
1982年 | 37篇 |
1981年 | 29篇 |
1980年 | 23篇 |
1979年 | 19篇 |
1978年 | 15篇 |
1977年 | 25篇 |
1976年 | 18篇 |
1974年 | 9篇 |
1973年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
A Collick J Drew J Penberth P Bois J Luckett F Scaerou A Jeffreys W Reik 《The EMBO journal》1996,15(5):1163-1171
Various sequences in the mammalian genomes are unstable. One class of sequence arrangement is long inverted repeats, which are known to be unstable in bacteria and yeast. While in mammals some evidence suggests that short inverted repeats (<10 bp long) may show instability, nothing is known about the stability of long inverted repeats. Here we describe two unrelated multicopy transgenes in the mouse (loci 109 and OX1-5), each of which contains a long inverted repeat that shows substantial mitotic instability. This instability also occurs in the germline so that mutant transgenes appear within pedigrees at a high frequency. The mutation processes acting at these two inverted repeats are complex and can involve insertion or deletion, and can result in stabilization of the transgene. At transgene 109 mutational events range from very small rearrangements at the centre of the inverted repeat to complete transgene deletion. In addition we show that the rates of mutation at the inverted repeat of transgene OX1-5 can vary between the male and female germlines and between inbred strains of mice, suggesting the possibility of a genetic analysis to identify loci that modulate inverted repeat instability. 相似文献
62.
63.
64.
Use of Genetic and Physical Mapping to Locate the Spinal Muscular Atrophy Locus between Two New Highly Polymorphic DNA Markers 总被引:9,自引:2,他引:7
下载免费PDF全文
![点击此处可从《American journal of human genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Olivier Clermont Philippe Burlet Lydie Burglen Suzie Lefebvre Fabrice Pascal John McPherson John J. Wasmuth Daniel Cohen Denis Le Paslier Jean Weissenbach Mark Lathrop Arnold Munnich Judith Melki 《American journal of human genetics》1994,54(4):687-694
The gene for autosomal recessive forms of spinal muscular atrophy (SMA) has recently been mapped to chromosome 5ql3, within a 4-cM region between the blocks D5S465/D5S125 and MAP-1B/D5S112. We identified two new highly polymorphic microsatellite DNA markers—namely, AFM265wf5 (D5S629) and AFM281yh9 (D5S637)—which are the closest markers to the SMA locus. Multilocus analysis by the location-score method was used to establish the best estimate of the SMA gene location. Our data suggest that the most likely location for SMA is between locus D5S629 and the block D5S637/D5S351/MAP-1B/D5S112/D5S357. Genetic analysis of inbred SMA families, based on homozygosity by descent and physical mapping using mega-YACs, gave additional information for the loci order as follows: cen–D5S6–D5S125/D5S465–D5S435–D5S629–SMA–D5S637–D5S351–MAP–1B/D5S112–D5S357–D5S39–tel. These data give the direction for bidirectional walking in order to clone this interval and isolate the SMA gene. 相似文献
65.
Starvation-Induced Stress Resistance in Lactococcus lactis subsp. lactis IL1403 总被引:2,自引:1,他引:1
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Axel Hartke Sandrine Bouche Xavier Gansel Philippe Boutibonnes Yanick Auffray 《Applied microbiology》1994,60(9):3474-3478
Carbohydrate-starved cultures of Lactococcus lactis subsp. lactis IL1403 showed enhanced resistance to heat, ethanol, acid, osmotic, and oxidative stresses. This cross-protection seems to be established progressively during the transitional growth phase, with maximum resistance occurring when cells enter the stationary phase. Chloramphenicol or rifamycin treatment does not abolish the development of a tolerant cell state but, on the contrary, seems to provoke this response in L. lactis subsp. lactis. 相似文献
66.
Murielle Rinaldi-Carmona Francis Barth Michel Haulme David Shire Bernard Calandra Christian Congy Serge Martinez Jeanne Maruani Gervais Nliat Daniel Caput Pascual Ferrara Philippe Soubri Jean Claude Brelire Grard Le Fur 《FEBS letters》1994,350(2-3):240-244
SR141716A is the first selective and orally active antagonist of the brain cannabinoid receptor. This compound displays nanomolar affinity for the central cannabinoid receptor but is not active on the peripheral cannabinoid receptor. In vitro, SR141716A antagonises the inhibitory effects of cannabinoid receptor agonists on both mouse vas deferens contractions and adenylyl cyclase activity in rat brain membranes. After intraperitoneal or oral administration SR141716A antagonises classical pharmacological and behavioural effects of cannabinoid receptor agonists. This compound should prove to be a powerful tool for investigating the in vivo functions of the anandamide/cannabinoid system. 相似文献
67.
A cytogenetic map of chromosome 5 from the Mediterranean fruit fly, Ceratitis capitata, was constructed. Six mutations were located by translocation, transposition or deletion mapping. This knowledge allowed alignment and orientation of the existing linkage map with the polytene chromosomes. In addition, mapping of mutations used as selectable markers in genetic sex-separation strains is an essential prerequisite for the improvement of genetic stability during mass-rearing. 相似文献
68.
69.
Leopold J. Anghileri Philippe Maincent Alfredo Cordova Martinez Pierre Thouvenot 《Biological trace element research》1994,46(1-2):103-111
The effects of the interaction between low molecular weight iron complexes (citrate, lactate, and ATP complexes) with ATP and proteins, on the modification of Ehrlich carcinoma cell calcium homeostasis have been studied. In that modification the ferric-ATP complex shows much higher activity than the others. Sodium ATP, by iron translocation from citrate and lactate, increases their activity. This phenomenon implicates ATP as a mediator on the cellular activity of the complexes. Proteins, particularly ferritin, appear to moderately reduce their activity, whereas glutathione and ascorbic acid, acting as lipid peroxidation-inhibitors, show only a slight reduction of the iron complex’s effects on cellular calcium uptake. 相似文献
70.
The effect of exogenous abscisic acid, provided to somatic embryos during the maturation step, on endogenous abscisic acid and its main conjugated form (abscisic acid glucose ester), germination and conversion frequencies is presented in this paper. Abscisic acid measurements were obtained after a methanolic extraction, a fractionation through high performance liquid chromatography, quantitation with an immunoassay and identification of the quantitated compound using gas chromatography-mass spectrometry. Results show that endogenous abscisic acid and abscisic acid glucose ester levels are clearly correlated with the exogenous abscisic acid concentration provided to the embryos. Maturation was clearly enhanced by exogenous abscisic acid, but no correlation was found between abscisic acid concentration and germination frequency. Conversely, development of the aerial part of the germinated somatic embryos was dependent upon the abscisic acid concentration in the culture medium and results suggest that this dependence could be related to the endogenous abscisic acid content. 相似文献