Studies on the biology of Spirorbinae (Polychaeta)

Of four species studied, tolerance of extreme temperatures in greatest in Spirorbis pagenstecheri (which extends highest on the shore) and least in S. corallinae (which always occurs immersed in water). The latter species breeds between May and August, the other three from May to October. S. borealis liberates its larvae mainly at the moon's quarters, but this fortnightly rhythm is less obvious in S. corallinae and not found in the other two species. S. corallinae differs from S. borealis in being slower to re-emerge after disturbance and in having a lesser breeding size, maximum size and life span. Growth in the laboratory is slower in S. tridentatus than in the other species. Growth of S. borealis under natural conditions is much faster in summer than in winter.     

Lesiones histoplasmóticas autópsicas como índice epidemiológico de la enfermedad en Los Andes Venezolanos

Resumen En 244 autopsias de los años 1961, 1962 y 1963 de sujetos de más de 2 años de edad fué encontrado un 25% de casos con lesiones histoplasmóticas, en su gran mayoría de carácter residual, en pulmones y ganglios linfáticos. Esta cifra corresponde exactamente al porcentaje de reactores positivos a la prueba de histoplasmina en la población de nuestra región. Fueron corroborados los resultados deStraub &Schwarz, quienes en Cincinnati/Ohio encontraron la misma concomitancia entre los resultados de estos métodos de estudio, ambos igualmente valiosos para determinar el indice epidemiológico de histoplasmosis en una zona determinada.Puesto que en nuestros estudios no fueron aplicadas las mismas técnicas completas como en Cincinnati las cifras positivas en nuestro ambiente pueden eventualmente ser más altas, pero no alcanzan las cifras aún más elevadas, reportadas de otras zonas de Venezuela. En nuestro material el número de casos con lesiones histoplasmóticas antiguas múltiples en el pulmón, que sugieren ser primarios, era mayor que en la primera serie deStraub &Schwarz. Parece que entre nosotros la infección tiene lugar en períodos más tempranos de la vida que en EEUU de Norteamérica.El más alto índice de infección, de casi 50%, fué encontrado en personas provenientes de la región de la Cuenca del Lago de Maracaibo, disminuyendo algo en los valles de baja altura de las montañas y llegando a cifras bajas en los lugares de mayor altura.

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Summary In 244 autopsies in patients from 2 years of age on, in 1961, 1962 and 1963 histoplasmic lesions were found in 25% of all cases, mostly residual foci in lungs and lymph nodes. This percentage is exactly the same as the percentage of histoplasmin sensitivity in our environment. Thus, the results ofStraub &Schwarz who found the same percentage of anatomic lesions and histoplasmin reactors in Cincinnati, Ohio, were confirmed in our material. Morphologic methods in autopsies are a valuable tool for epidemiologic investigations regarding histoplasmosis.Since a more exact technique of examination was applied in Cincinnati, it is probable that the positive percentage in our environment is even higher. A higher number of multiple residual histoplasmotic foci in the lungs was found in our series in comparison with the first material ofStraub &Schwarz. Multiple histoplasmic foci in some of these cases may be due to primary infection. Infection withH. capsulatum occurs apparently in our environment more frequently in younger persons.The highest infection rate of almost 50% was found in patients who lived in the tropical lowlands near the Lake of Maracaibo and the Panamerican Highway. A lower rate occurred in the mountain valleys up to approximately 3000 feet of altitude, and decreased even more in higher altitudes.

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Zusammenfassung In 244 Sektionen vom 2. Lebensjahr an aus den Jahren 1961, 1962 und 1963 wurde in 25% aller Fälle Histoplasmose in Form von Residualläsionen in Lungen und Lymphknoten gefunden. Diese Zahl entspricht genau dem Prozentsatz der Bevölkerung in unserer Gegend, die positiv auf Histoplasmin reagiert. Damit werden die Befunde vonStraub &Schwarz, die an Autopsien in Cincinnati, Ohio erhoben wurden, bestätigt. Neben der Histoplasminreaktion ist es auch mittels anatomischer Untersuchungen möglich, Aussagen über das Ausmass der Infektion mitHistoplasma capsulatum in einer bestimmten Gegend zu machen.Da bei den Untersuchungen in Cincinnati genauere Untersuchungsmethoden zur Anwendung kamen, ist es möglich, dass der Prozentsatz unserer positiven Fälle eher noch höher liegt. Die Zahl unserer Fälle mit multiplen residualen Histoplasmoseherden in den Lungen ist noch höher als in der ersten Untersuchungsserie vonStraub &Schwarz. Es könnte sich in einem Teil der Fälle durchaus um multiple Primärkomplexherde handeln. Anscheinend findet die Infektion mitH. capsulatum bei uns in früheren Lebensaltern statt.Die höchste Infektionsrate von fast 50% wurde in Personen aus den gegen den Maracaibosee zu gelegenen tropischen Gebieten gefunden. Sie war etwas geringer in den Gebirgstälern bis zu etwa 1000 m Höhe und gering in Gebieten grösserer Höhe.

     

A Rapid Screening Test for Aflatoxin-synthesizing Aspergilli of the flavus-oryzae Group

A rapid test for the recognition of aflatoxin-synthesizing strains of the Aspergillus flavus–oryzae group is described. For this purpose the strains are cultivated on Czapek–Dox agar enriched with an aqueous extract of groundnuts, and in which sodium nitrate is replaced by ammonium chloride. Toxin production is observed by the production of a bright blue fluorescence in the medium when placed under an ultraviolet lamp.     

Direct effects of serotonin and ketanserin on the functional morphology of embryonic chick skin in vitro

Summary Different organotypical culture methods are used to test the direct effects of serotonin and ketanserin, a S₂, α₁, and H₁ receptor antagonist in vascular tissue, on fibroblasts and epidermal cells of embryonic chick skin in vitro. From light microscopic and electron microscopic analyses, we learn that serotonin enhances keratinization and differentiation, whereas ketanserin reduces differentiation in comparison to the control cultures. Incorporation data of fragments cultured with [³H]thymidine show that ketanserin, within a dose range from 0.05 to 5 μg/ml, stimulates proliferation. Serotonin at a concentration of 10 μg/ml slightly slows down proliferation, whereas lower doses of 0.1 and 1 μg/ml result in tritium activities that do not differ from control cultures. This investigation was financially supported by the National Fund of Scientific Research, Belgium, 3.0022.87.     

Characterization of human mammary cell types in primary culture: Immunofluorescent and immunocytochemical indicators of cellular heterogeneity

Summary Parenchymal organoidal structures that were obtained from collagenase digestion of reduction mammoplasty specimens of apparently normal human breasts have been grown in short-term primary cultures, either on plastic or on floating gels of polymerized rat-tail collagen. Three morphologically distinct major cell types are readily observed in both systems: cuboidal cells, which occupy apical positions on collagen gels; larger, epithelioid, or basal cells on gels; and elongated cells which penetrate into the gel. In addition, a fourth cell type, that of a large, flat cell, is observed less readily by phase contrast microscopy on the surface of cultures grown on plastic. Immunofluorescent and immunocytochemical staining of cultures on plastic or histologic sections of cultures on gels have been undertaken with antisera and other histochemical reagents that stain the different parenchymal cell types in vivo. Thus antisera to epithelial membrane antigen(s), monoclonal antibodies (MABs) to the defatted mammary milk fat globule membrane, peanut lectin, and keratin MAB LE61, which preferentially stain the epithelial cells of ducts in vivo, also stain the cuboidal/apical cells in vitro. The large, flat cells are stained intensely by the first three reagents but not by the last one. Antisera to collagen IV, laminin, fibronectin, actin, keratin MAB LP34, MABs to the common acute lymphoblastic leukemia antigen, and MAB LICR-LON-23.10, which showed enhanced staining for the ductal myoepithelial cells in vivo, also stain the epithelioid/elongated cells in vitro. However, the effect of the last four reagents is reduced considerably in most elongated cells, and MAB LP34 stains the large, flat cells intensely. Heterogeneous cells of intermediate morphologies and staining patterns between the cuboidal/flat cells and large epithelioid cells have also been identified. The results suggest that the cuboidal cells and large, flat cells are related to mammary epithelial cells, whereas the large epithelioid/elongated cells have some characteristics of myoepithelial cells, and that intermediate forms may exist in culture between the two parenchymal cell types. This work was supported in part by the Ludwig Institute for Cancer Research and the Cancer and Polio Research Fund. Dr. M. J. Warburton is supported by the Cancer Research Campaign.     

Influence ofl-thyroxine,l-triiodothyronine,thyroid stimulating hormone,or estradiol on the cell kinetics of cultured mammary cancer cells

Summary We describe the in vitro influence of 3,5,3′-triiodo-l-thyronine (T₃),l-thyroxine (T₄), a thyroid-stimulating hormone (TSH), and/or estradiol (E₂: chosen as the control of the methodology) on the cell kinetics (cell distribution in the S+G₂+M phases) of mouse MXT and human MCF-7 mammary cancer cells. Experiments were performed by means of a cell image processor, analyzing MCF-7 or MXT cells that had been grown on glass cover slips and whose nuclei had been stained by the Feulgen reaction, which is selective and quantitative (stoichiometric) with respect to DNA. We show that T₃, T₄, and TSH at 0.01 μM dramatically stimulate the cell kinetics of the MXT mouse and the MCF-7 human mammary cancer cell lines. Indeed, the three hormones bring about a significant transient increase in the S+G₂+M fraction as does E₂. Furthermore, our data indicate that E₂ and TSH are antagonistic with regards to MXT or MCF-7 cell kinetics. This work is supported by grants awarded by the IRSIA and the Fonds de la Recherche Scientifique Médicale (FRSM, Belgium).     

Monitoring juvenile stocks of flatfish in the Wadden Sea and the coastal areas of the southeastern North Sea

Results are presented of an on-going monitoring program, started in 1970, of the demersal fish population in the Wadden Sea, Scheldt estuary, and along the continental coast between the Belgian-French border and Esbjerg. Particular attention is given to long-term trends in overall abundance and annual variations in spatial distribution of 0- and 1-group plaice and sole in relation to year-class strength and to variations in growth rate. Recruitment level in both plaice and sole appeared to be higher in the 1980s than in the 1970s. The most important nursery areas for plaice are the German Bight and the Wadden Sea, and the relative contributions of these areas to the total plaice stock in different years are relatively stable. In contrast, the most important nurseries for sole are along the continental coast, and the contribution of different parts of these nurseries vary considerably from year to year. Abundant year-classes of sole can originate from northern as well as from southern parts of the nurseries. Relations between survey results and year-class estimates from Virtual Population Analysis (VPA) for plaice are better compared to sole. No trends have been observed in mean length at age 0 and 1 during the years the surveys have been carried out. It is concluded that the quality of the continental coastal area and the Wadden Sea as nursery area for plaice and sole has not declined during the period of investigation. Presented at the VI International Wadden Sea Symposium (Biologische Anstalt Helgoland, Wattenmeerstation Sylt, D-2282 List, FRG, 1–4 November 1988)